【DNA細胞轉染試劑】多聚陽離子(polycations)和脂質體(liposomes)配方-ABMG2100
DNAfectin™2100 Transfection Reagent | 貨號G2100 1ml (1.0mg/1.0ml )
DNAfectin™2100 Transfection Reagent是一種獨特的多聚陽離子(polycations)和脂質體(liposomes)配方，可以高效，有效地轉染真核細胞(eukaryotic cells)。 DNAfectin™是超過15種不同細胞系的最佳轉染試劑，包括難以轉染的細胞，懸浮細胞 (suspension)和原代細胞(primary cells)。 這種轉染試劑非常適合任何轉染需求。5ug DNA and 10ul of transfection reagent will be fine. If toxicity is a problem, use 3~4 ug DNA。
DNAfectin™ Transfection Reagent is a special and unique formulation of polycations, offering a high transfection efficiency with low cytotoxicity for many cell types, including primary cells. In contrast, DNAfectin™ Plus is a nanoparticle-based transfection reagent. This innovative technology allows DNAfectin™ Plus to achieve superior and reproducible transgene expression levels with no cytotoxic effects. DNAfectin™ Plus also offers a simple protocol that is serum-compatible.
| CELL LINE | CELL LINE SPECIES/TISSUE | NUCLEIC ACID | REAGENT | TRANSFECTION TYPE | TRANSFECTION EFFICIENCY(%) |
|---|---|---|---|---|---|
| 293T | Human Embryonic Kidney | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 100% |
| Jurkat | Human T Lymphoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 25% |
| SHEP | Human Neuroblastoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 90% |
| BOSC23 | Human Kidney | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 90% |
| HT1080 | Human Fibrosarcoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 80% |
| C33A | Human Cervical Carcinoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 90% |
| MRC5 | Human Primary Fibroblast | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 80% |
| MDK-Telo | Dog Kidney Cells+Telo | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 80% |
| B16 | Murine Melanoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 80% |
| CB3 | Mouse Erytholeukemia | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 25% |
| HuVEC | Human Ubilical Vein | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 20% |
| HepG2 | Human Liver Carcinoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 40% |
| MMRU | Human Melanoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 75% |
| MMAN | Human Melanoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 80% |
| MDA-MB-231 | Human Breast Carcinoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 85% |
| HeLa | Human Cervical Carcinoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 80%-90% |
| A2780 | Human Ovarian Carcinoma | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 70%-75% |
| L6 | Rat Myoblast | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 60%-70% |
| Rabbit-telo | Telomerase-Rabbit Cells | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 50% |
| USO2 (T-USO2) | Human Osteosarcoma (tet-inducible) | DNA | DNAfectinTM 2100 Transfection Reagent | Transient | 80%-90% |
黴漿菌PCR偵測 | DAPI染核 | 細胞內黴漿菌去除劑 | 水浴槽抑菌劑 | 噴霧式去黴劑 等系列商品