【BlockPRO™無蛋白封閉緩衝液】Protein free blocking buffer Visual Protein BF01-1L
【blocking封閉緩衝液】非蛋白質配方-有效增加訊號及最小化背景值
轉印膜 (transfer membrane)和抗體反應之前，須進行封閉(blocking)，以封閉 (blocking) 轉印膜上蛋白質外空白區域，減少抗體 (蛋白質) 吸附，造成之背景值增加。傳統方法常用0.05%-0.5%的BSA、10% 的小牛血清、1%明膠(gelatin)、3-5% non-fat milk (配在 TBST 或 PBST 裡)，進行封閉(blocking)。若blocking中的蛋白質被目標抗體辨認，則造成背景值增加。Visual protein 提供的 BlockPRO Protein-Free Blocking Buffer封閉緩衝液是一種非蛋白質配方，可以有效地增加訊號，最小化背景值；應用廣泛，適用於 Western blotting 與 ELISA 等免疫學實驗。
BlockPRO™ Protein-Free Blocking Buffer | 貨號BF01-1L
產品介紹: BlockPRO™不含蛋白質的封閉緩衝液是一種非蛋白質配方，能有效降低遮蓋液與蛋白樣本的交互作用，避免物種干擾。可提高靈敏度並最小化背景噪音，在免疫測定中比傳統的基於蛋白質的封閉緩衝液呈現更好的結果。 BlockPRO™無蛋白封閉緩衝液的合成配方使其適用於PVDF和硝酸纖維素平台，抗生物素蛋白/生物素系統，磷蛋白檢測以及其他免疫化學應用。應用廣泛，適用於 Western blotting 與 ELISA 等免疫學實驗。
產品特點:
- 高性能：比傳統的阻塞緩衝器提供更好的特定信號和更少的背景噪聲
- 多種應用：適用於蛋白質印跡，斑點印跡，ELISA和其他免疫測定
- 高質量控制：確保批次間的一致性，以確保長期獲得最可重複的結果
Figure 1. BlockPRO™ Protein-Free Blocking Buffer is better than protein-based blocking buffers (skim milk, BSA and casein) for detection of target protein in Western blotting. THP-1 cell lysates were prepared and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 hour at room temperature with the indicated blocking buffer, probed with mouse anti-pAMPK followed by anti-mouse HRP and detected by chemiluminescence. All results were exposed to X-ray film for 30 seconds.
Figure 2. BlockPRO™ Protein-Free is suitable for various protein antigen detection, such as high molecular weight protein, tACC; phosphoprotein, pAMPK; abundant protein, GAPDH; low molecular weight protein, histone H3. THP-1 cell lysates were prepared and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 hour at room temperature with BlockPRO™ Protein-Free Blocking Buffer. Antibodies designed to probe the indicated proteins were used. All the signals were detected by chemiluminescence and were exposed to X-ray film.
Figure 3. BlockPRO™ Protein-Free Blocking Buffer can be used in both PVDF and nitrocellulose platform. Hela cell lysates were prepared and separated by SDS-PAGE. The proteins were transferred to PVDF or nitrocellulose membranes. The membranes were blocked for overnight at 4 °C with BlockPRO™ Protein-Free Blocking Buffer or 5% skim milk, probed with mouse anti-histone H3 followed by anti-mouse HRP and detected by chemiluminescence. All results were exposed to X-ray film for 30 seconds.
| Cat. No. | Product Name | Description |
| BF01-1L | BlockPRO™ Protein-Free Blocking Buffer |
1kit -500mL Solution X 2
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BF10-100
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BlockPRO™ Protein-Free Blocking Buffer (10X) | 1kit – 100mL 10X Solution X 1 |
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BF10-200
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BlockPRO™ Protein-Free Blocking Buffer (10X) | 1kit – 100mL 10X Solution X 2 |
| BR01-500 | Bradford 蛋白質定量試劑 | Dual-Range Bradford Reagent (1X), 500 ml , | |
| BR05-500 | 5X Bradford 蛋白質定量試劑 | Dual-Range Bradford Reagent (5X), 500ml | |
| BR05-500-K | 5X Bradford 蛋白質定量試劑組 |
Dual-Range Bradford Protein Assay Kit, (500 ml +10* 2mg/ml BSA standard) |
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| BP01-1L | 西方墨點法遮蓋液 | BlockPRO Blocking Buffer, 500mlx2 | |
| BF01-1L | 西方墨點法遮蓋液-Protein Free | BlockPRO Protein-Free Blocking Buffer, 500mlx2 | |
| BF20-50P | 西方墨點法遮蓋液-Protein Free |
BlockPRO Protein-Free Blocking Buffer, 20X, 20包，每包2.5 mL， 使用前加入500mL ddH2O 回溶成 1X |
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| 西方墨點法遮蓋液-Protein Free, 20X | |||
| BM01-500 | 西方墨點法遮蓋液-1分鐘配方 | BlockPRO 1 Min Protein-Free Blocking Buffer, 500ml | |
| SP01-500 | 抗體去除試劑 | StripPRO 1 Min Stripping Buffer, 500ml | |
| LF01-500 | 冷光試劑組- Prime | LumiFlash Prime Chemiluminescent Substrate,( 250mL solnA + 250mL soln B) | |
| LF08-500 | 冷光試劑組- Ultima | LumiFlash Ultima Chemiluminescent Substrate, (250mL solnA + 250mL soln B) | |
| LF16-500 | 冷光試劑組- Infinity | LumiFlash Infinity Chemiluminescent Substrate, (250mL solnA + 250mL soln B) | |
| VC01-250 | 預染蛋白質標準液 | VisColorTM Pre-Stained Protein Marker, 250ul x 1 | |
| VC01-500 | 預染蛋白質標準液 | VisColorTM Pre-Stained Protein Marker, 250ul x 2 | |
| VC03-250 | 全範圍預染蛋白質標準液 |
VisColorTM Full-Range Pre-Stained Protein Marker, 250ul x 1
|
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| VC03-500 | 全範圍預染蛋白質標準液 |
VisColorTM Full-Range Pre-Stained Protein Marker, 250ul x 2
|
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| LH03-10 | 冷光筆 |
LuminolPen HRP system, (約 150 張 Western blot’s membranes 使用) |
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| LH03-50 | 冷光筆 |
LuminolPen HRP system, (約1000 張 Western blot’s membranes 使用) |
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| LH05-10 | 冷光筆 EZ版 | LuminolPen EZ HRP System, (約 150 張 Western blot’s membranes 使用) | |
| LH05-50 | 冷光筆 EZ版 | LuminolPen EZ HRP System, (約 1000 張 Western blot’s membranes 使用) | |
| RB10-1L | SDS-PAGE 電泳緩衝液 | SDS-PAGE Running Buffer , 10X (Tris-Glycine, pH 8.3), 500 ml *2 | |
| RB500P | SDS-PAGE 電泳緩衝液濃縮包 |
SDS-PAGE Running Buffer , 10X (Tris-Glycine, pH 8.3), (20包，每 包須於使用前加入500mL ddH2O 回溶成 1X) |
|
| WTB10-1L | Western Blot Transfer Buffer | Western Blot Transfer Buffer, 10X (Tris-Glycine, pH 8.3), 500 ml *2 西方墨點法轉印緩衝液 | |
| WTB500P | 西方墨點法轉印緩衝液濃縮包 |
Western Blot Transfer Buffer,(20包，每包須於使用前加入500mL ddH2O 回溶成 1X) |
|
| TBS10-1L | TBS緩衝液 | TBS Buffer, 10X, 500 mL*2 | |
| TBS200P | TBS 緩衝液濃縮包 |
TBS Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) |
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| TBST10-1L | TBST 緩衝液 | TBS Tween-20 Buffer, 10X, 500 mL*2 | |
| TBST200P | TBST 緩衝液濃縮包 |
TBS Tween-20 Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) |
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| PBS10-1L | PBS 緩衝液 | PBS Buffer, 10X , 500 mL*2 | |
| PBS200P | PBS 緩衝液 | PBS Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) | |
| PBST10-1L | PBST 緩衝液 | PBS Tween-20 Buffer, 10X, 500 mL*2 | |
| PBST200P | PBST 緩衝液 |
PBS Tween-20 Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) |
|
| SBN06-15 | 6x Laemmli SDS Sample Buffer, non-reducing | 6x Laemmli SDS Sample Buffer, non-reducing, 15ml | |
| SBR06-15 | 6x Laemmli SDS Sample Buffer, reducing | 6x Laemmli SDS Sample Buffer, reducing, 15ml | |
| VP01-125 | 全蛋白質負染色試劑組 |
VisPRO 5 Minutes Protein Stain Kit, (125mL soln 1 + 125mL soln2) |
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| VP01-500 | 全蛋白質負染色試劑組 |
VisPRO 5 Minutes Protein Stain Kit, (500mL soln 1 + 500mL soln 2) |
|
| VP05-125 | 全蛋白質負染色試劑組- 五倍濃縮液 |
5x VisPRO 5 Minutes Protein Stain Kit, (125mL soln 1 + 125mL soln 2)
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| VP05-500 | 全蛋白質負染色試劑組- 五倍濃縮液 | 5x VisPRO 5 Minutes Protein Stain Kit, (250mL soln 1 + 250mL soln 2) | |
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PP03-2C
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磷酸化蛋質萃取試劑組 | PhosPRO Phosphoprotein Enrichment Kit, (2 pre-packed column + 2mL Lysis buffer+250mL System buffer) +(100mL Elution buffer+100 mL Stringent buffer stock) | |
| PP03-6C | 磷酸化蛋質萃取試劑組 |
PhosPRO Phosphoprotein Enrichment Kit, (6 pre-packed column + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock ) |
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PP03-5E
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磷酸化蛋質萃取試劑組 |
PhosPRO Phosphoprotein Enrichment Kit, (3 mL Resin + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock) |
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PP05-2C
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PhosPRO Phosphoprotein Purification Kit, (2 pre-packed column + 2mL Lysis buffer+250mL System buffer) +(100mL Elution buffer+100 mL Stringent buffer stock) | ||
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PP05-6C
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磷酸化蛋白質純化試劑組 | PhosPRO Phosphoprotein Purification Kit, (6 pre-packed column + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock) 磷酸化蛋白質純化試劑組 | |
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PP05-5E
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磷酸化蛋白質純化試劑組 | PhosPRO Phosphoprotein Purification Kit, (3 mL Resin + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock ) | |
| EP05-30 | 全蛋白質萃取試劑 | ExtractPRO Protein Extraction Reagent | |
| FP01-5 | 胞器蛋白質分離試劑組 | FracPRO Protein Fractionation Kit, (10mL Soln1+ 10mL Soln2 +7.5mL Soln3+1.5mL Soln4) | |
| FP01-50 | 胞器蛋白質分離試劑組 | FracPRO Protein Fractionation Kit, (100mL Soln1 + 100mL Soln2+ 75mL Soln3 +15mL Soln4) | |
| HB01-1L | 促融合瘤細胞生長因子 | HybriMore, Hybridoma cloning factor, for 1L medium dilution | |
| IF01-4N | 快速免疫佐劑 | ImmunoFast Adjuvant, for 4 injections to mouse | |
| DG03-1P | DIGE蛋白質標記試劑組 | DIGE-Kit, (Cy2 400pmole + Cy3 400pmole + Cy5 400pmole+ 20ml DMF) | |
| GLP01 | 負染膠片光箱 | Gel Lighting Plate 21 x 29.7 cm(A4) | |
| BLP01 | 正染膠片光箱 | Band Lighting Plate 21 x 29.7 cm(A4) | |
| BSB01 | 黑染盒 | Black Staining Box 13 x 13 x 4 cm (For mini gel) | |
| BSB02 | 黑染盒 | Black Staining Box 17.5 x 17.5 x 4.5 cm (For regular gel) | |
| BSB03 | 黑染盒 | Black Staining Box 20 x 24 x 5 cm | |
| BSB04 | 黑染盒 | Black Staining Box 27 x 27 x 5 cm |