ATPase/GTPase Assay Kit | abnova 貨號KA1610

靈敏度6.25 to 400 U/mL

ATP 酶和 GTP 酶催化 ATP 或 GTP 分解為 ADP 或 GDP 和游離磷酸根離子(free phosphate ion)。這些酶在運輸( transport)、信號轉導(signal transduction)、蛋白質生物合成(protein biosynthesis)和細胞分化(cell differentiation)中起著關鍵作用。ATPase/GTPase 檢測試劑盒提供了一種高度靈敏的方法來確定 ATPase/GTPase 活性。其專有配方具有單一試劑，可準確測定酶室溫 30 分鐘內活性。改進後的孔雀石綠試劑(malachite green reagent )形成穩定的墨綠色( dark green color)釋放的磷酸鹽( liberated phosphate)，在讀板器 (600 – 660 nm) 上測量。

ATPase/GTPase Assay Kit應用：
• ATPase 和GTPase 活性的測定。
• 藥物發現：ATPase 或GTPase 抑製劑的高通量篩選。

ATPase/GTPase Assay Kit產品特點
• 高靈敏度：檢測0.007 U/L ATPase 或GTPase 活性。
• 快速方便：單一試劑、均質的“混合測量”30 分鐘內定量酶活性。
• 穩健且適用於 HTS：620 nm 檢測大大降低了有色物質的潛在干擾化合物。在 96 孔板和 384 孔板中觀察到 >0.7 的 Z’ 因子。可以很容易地自動化HTS 液體處理系統。

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