CCK-8細胞增殖和細胞毒性試劑盒 | 貨號 KTC011001

CCK-8 Cell counting/proliferation KIT

CCK-8; WST-8; CCK8; WST8

產品描述：Abbkine Cell Counting Kit-8（CCK-8）設計用於檢測細胞增殖和細胞毒性。細胞計數試劑盒-8（CCK-8）使用高度水溶性的四唑鹽WST-8：[2-（2-甲氧基-4-硝基苯基）-3-（4-硝基苯基）-5-（2,4-二磺基苯基 ）-2H-四唑鎓單鈉鹽]通過還原轉化為水溶性橙色甲dye染料。 CCK-8具有低毒性，並且允許用於測定細胞增殖和細胞毒性測定中活細胞數量的靈敏比色測定。 細胞中脫氫酶產生的甲dye染料的量與活細胞的數量成正比。

 

• Formulation

Liquid solution

• Kit components
• CCK-8 solution
• Features & Benefits
• One-bottle, ready-to-use solution.
  • The product (formazan) produced by WST-8 is water soluble, No organic solvents or isotopes required. And the formazan is stable and safe.
  • The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
  • The same cells can be used for other cell assays because of the low toxicity of CCK-8.
• Storage instructions

CCK-8 is stable over one year at 0-5°C with protection from light. Store it at -20°C for longer storage. Repeated thawing and freezing causes an increase in the background, which interferes with the assay. Please store the kit at 0-5°C for frequent use.

• Shipping

Gel pack with blue ice.

注意事項

• 小心不要將氣泡引入Well，因為它們會干擾O.D. 讀。
• 孵育時間因細胞的類型和數量而異。 通常，白細胞的著色較弱，因此可能需要較長的培養時間（長達4小時）或大量細胞（〜105細胞/孔）。
• 如果培養基的顏色或PH由於長期培養而改變，請在添加CCK-8時更換培養基。

引用文獻

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10. Magnolin inhibits IgE/Ag-induced allergy in vivo and in vitroP Ma, D Che, T Zhao, Y Zhang, C Li, H, An. International Immunopharmacology, Volume 76, November 2019.
11. Dehydroandrographolide inhibits IgE-mediated anaphylactic reactions via calcium signaling pathwayD Che, Y Hou, Y Zeng, C Li, Y Zhang, D Wei. Toxicology and Applied Pharmacology, Volume 366, 1 March 2019, Pages 46-53.
12. Aqp-1 Gene Knockout Attenuates Hypoxic Pulmonary Hypertension of MiceM Liu, Q Liu, Y Pei, M Gong, X Cui, J Pan. Arteriosclerosis, Thrombosis, and Vascular Biology, 2019.
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14. Target Proteins of Phloretin for Its Anti-Inflammatory and Antibacterial Activities Against Propionibacterium acnes-Induced Skin InfectionDasom Cheon, Jieun Kim, Dasom Jeon, et al. Molecules. 2019 Apr 3;24(7).
15. Aqp-1 Gene Knockout Attenuates Hypoxic Pulmonary Hypertension of MiceMingcheng Liu, Qiwang Liu, Yandong Pei, et al. Arterioscler Thromb Vasc Biol. 2019 Jan;39(1):48-62.
16. LPS-induced CXCR7 expression promotes gastric Cancer proliferation and migration via the TLR4/MD-2 pathwayNan Li, Huanbai Xu, Yurong Ou, et al. Diagn Pathol. 2019 Jan 12;14(1):3.
17. Dehydroandrographolide inhibits IgE-mediated anaphylactic reactions via calcium signaling pathwayDelu Che, Yajing Hou, Yingnan Zeng, et al. Toxicol Appl Pharmacol. 2019 Mar 1;366:46-53.
18. Quercetin inhibits Mrgprx2-induced pseudo-allergic reaction via PLCγ-IP3R related Ca2+ fluctuationsYuanyuan Dinga, Delu Chea, et al. International Immunopharmacology. 2018; 66: 185-197.
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