蛋白A-瓊脂糖珠Protein A Sepharose | 貨號6501 (Abcam ab193256)

Reusable Protein A conjugated Sepharose beads with ≥16 mg/ml binding capacity & 2.07 ml/min flow rate for antibody purification from multiple sources

通過將重組蛋白A共價偶聯至6％交聯瓊脂糖珠來製備蛋白A-瓊脂糖珠 (≥16 mg/ml)。 BioVision蛋白A（Cat。＃6500，Cat。＃6500B）是一種基因工程蛋白，含有5個天然蛋白A的IgG結合區。細胞壁結合區，白蛋白結合區和其他非特異性區域已從 重組蛋白A以確保最大特異性IgG結合。 偶聯技術經過優化，可以提供更高的IgG重組蛋白A的結合能力和最小的浸出量。蛋白A-瓊脂糖的IgG結合能力≥每毫升濕珠≥16mg人或兔IgG。 蛋白A-瓊脂糖珠顯示出高的化學和物理穩定性以及高流速，親水性和高凝膠強度。 它可以用於IgG純化和免疫沉澱。

Highlights

• CONTENTS – Supplied as a 50% slurry in 20 % Ethanol/H2O. – HIGH BINDING CAPACITY: Binding of IgG ≥16 mg human or rabbit IgG/ml Protein A-Sepharose. – MINIMAL LEACHING OF THE LIGAND. – FLOW RATE TESTED*: 2.07 ml/min. *Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm). – USAGE: Reusable for up to 10 times without significant loss of binding capacity. • APPLICATIONS – Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. – Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.