細胞激素(Cytokines)-Human EGF protein重組蛋白

細胞激素(Cytokines)是一類獨特的細胞訊號傳導分子，在細胞增殖和發育中扮演重要角色。細胞因子Abbkine 提供細胞因子，包括白血球介素( interleukin)、趨化因子(chemokines)、集落刺激因子(colony-stimulating factors, CSF)、干擾素(interferon)以及轉化生長因子(transforming growth factor , TGF) 和腫瘤壞死因子(tumor necrosis factor , TNF) 家族，用於癌症(cancer)、細胞凋亡(apoptosis)、內分泌學(endocrinology)、免疫學(immunology)、神經科學(neuroscience)、蛋白酶(proteases)、和幹細胞研究。這些細胞激素經過全程品質管制，品質嚴格驗證，可以滿足科學家對高品質、純度高、內毒素含量低、高生物活性和有競爭力的價格的需求。

Human EGF protein重組蛋白 |貨號PRP100159

Publications(1) 

EGF 是 EGF 蛋白家族(EGF-family)的創始成員。該蛋白質家族的成員具有高度相似的結構(structural)和功能(functional)特徵。 EGF 包含 9 個 EGF 樣結構域(EGF-like domains)和 9 個 LDL 受體 B 類重複序列(LDL-receptor class B repeats)。人 EGF 是一種 6045-Da 的蛋白質，具有 53 個氨基酸殘基(amino acid residues)和三個分子內二硫鍵(disulfide bonds)。作為一種低分子多肽(low-molecular-weight polypeptide)，EGF最初是從小鼠頜下腺(submandibular gland)中純化而來，此後在包括頜下腺(submandibular gland)、腮腺(parotid gland)在內的多種人體組織中均有發現。它也存在於人類血小板(platelets)、巨噬細胞(macrophages)、尿液(urine)、唾液(saliva)、牛奶(milk)和血漿(plasma)中。 EGF 是一種生長因子(growth factor)，可在體內和體外刺激各種表皮(epidermal)和上皮組織(epithelial tissues)的生長，以及細胞培養物中某些成纖維細胞的生長。它導致細胞增殖(cellular proliferation)、分化(differentiation)和存活 (survival)。唾液 EGF(Salivary EGF) 似乎也受膳食無機碘(dietary inorganic iodine)的調節，在維持口食管和胃組織完整性方面也起著重要的生理作用。 EGF通過與細胞表面的表皮生長因子(epidermal growth factor receptor)受體以高親和力結合併刺激受體的內在蛋白酪氨酸激酶(intrinsic protein-tyrosine kinase)活性而起作用。反過來，酪氨酸激酶(tyrosine kinase)活性啟動信號轉導級聯，導致細胞內發生各種生化變化——細胞內鈣(calcium)水平升高、糖酵解和蛋白質合成(protein synthesis)增加，以及某些基因的表達增加，包括EGFR——最終導致 DNA 合成(DNA synthesis)和細胞增殖(cell proliferation)。

產品介紹: Human EGF protein重組蛋白。 表達和純化源自成熟形式的人 EGF (NP_001954.2) (Asn 971-Arg 1023) 的氨基酸序列，在 N 末端具有初始 Met。使用 Balb/C 3T3 小鼠胚胎成纖維細胞在細胞增殖試驗中測量。這種效果的 ED50 通常為 0.15-0.75 pg/mL。重組人EGF由54個氨基酸組成，在還原條件下在SDS-PAGE中估計的分子量為6.3 kDa。SDS-PAGE 測定 > 92 %。

Product name	Human EGF protein
Sequence	Amino acid sequence derived from mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was expressed and purified, with an initial Met at the N-terminus.
Activity	Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.15-0.75 pg/mL.
Protein length	The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.3 kDa as estimated in SDS-PAGE under reducing conditions.
Preparation method	E. coli
Purity	> 92 % as determined by SDS-PAGE
Alternative	Epidermal Growth Factor; HOMG4; URG; Pro-epidermal growth factor; EGF
Formulation	Lyophilized from sterile PBS, pH 7.4
Molecular weight	6.3 kDa
Usage notes	Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human EGF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions	Lyophilized Human EGF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.52% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping	The product is shipped at ambient temperature.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.