真菌蛋白(細胞壁)提取試劑盒(detergent-free) | Invent貨號YT-015

含有細胞壁的微生物(cell-wall-containing microbes) 中提取蛋白質是一種常見程序。YT-015提供了一種無需儀器，快速而溫和的方式，可從具有厚而堅固的細胞壁的微生物 (microbes) 中提取蛋白質。這些微生物包括酵母(yeast)，絲狀真菌(filamentous fungus)，革蘭氏陽性(gram-positive)和革蘭氏陰性細菌(negative bacteria)，昆蟲卵(insect eggs)，微藻類(microalgae)等。該試劑盒包含優化的變性(denaturing protein extraction buffers 貨號) 和天然蛋白質提取緩衝液 (native protein extraction buffers)供您選擇。與許多其他苛刻條件下的方法不同（例如8 M尿素），使用酵母進行儀器進行玻璃珠裂解並用鹼萃取將樣品煮沸等方法用於酵母蛋白質萃取。通常使用這些方法優先提取某些分子量範圍內的蛋白質。該試劑盒具有單管操作規程，可以無偏差地提取所有種類的酵母蛋白。該試劑盒適用於對數期(log-phase)和固定期(stationary-phase)之微生物。整個過程不到10分鐘即可完成，蛋白質產量在2-5 mg / ml的範圍內。提供的材料足以進行50次反應。

Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps)

適用於酵母(yeast)，絲狀真菌(filamentous fungus)，革蘭氏陽性(gram-positive)和革蘭氏陰性細菌(negative bacteria)，昆蟲卵(insect eggs)，微藻類(microalgae)等蛋白質萃取

Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性。無須長時間等待、免除重複性的wash步驟。

Kit includes:

Items	Quantity
Denaturing Buffer	20 ml
Native Buffer	20 ml
Protein Extraction Powder	5 grams
1.5 ml Microfuge Tube	50 units
Pestles	4 units

引用文獻

1. Nagayoshi, Y., Miyazaki, T., Shimamura, S., Nakayama, H., Minematsu, A., Yamauchi, S., … & Mukae, H. (2017). Unexpected effects of azole transporter inhibitors on antifungal susceptibility in Candida glabrata and other pathogenic Candida species. PloS one, 12(7), e0180990.
2. Al-Omari, A., Griffith, J., Caranica, C., Taha, T., Schuttler, H. B., & Arnold, J. (2018). Discovering regulators in Post-Transcriptional control of the Biological Clock of Neurospora crassa using Variable Topology Ensemble Methods on GPUs. IEEE access.
3. Terashima, M., Ohashi, K., Takasuka, T. E., Kojima, H., & Fukui, M. Antarctic heterotrophic bacterium Hymenobacter nivis P3T displays light‐enhanced growth and expresses putative photoactive proteins. Environmental Microbiology Reports.
4. Lin, W., Yuan, D., Deng, Z., Niu, B., & Chen, Q. The cellular and molecular mechanism of glutaraldehyde‐didecyldimethylammonium bromide as a disinfectant against Candida albicans. Journal of Applied Microbiology.
5. Shimamura, S., Miyazaki, T., Tashiro, M., Takazono, T., Saijo, T., Yamamoto, K., … & Mukae, H. (2019). Autophagy-Inducing Factor Atg1 Is Required for Virulence in the Pathogenic Fungus Candida glabrata. Frontiers in microbiology, 10.
6. Wang, Y., Ma, Y., Wang, D., Liu, W., Chen, J., Jiang, Y., … & Qin, L. (2019). Polar tube structure and three polar tube proteins identified from Nosema pernyi. Journal of Invertebrate Pathology, 107272.

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