【改良的革蘭氏染色試劑盒Gram Stain Kit】Scytek貨號BBS-2
Gram Stain Kit (MODIFIED BROWN AND BRENN) | BBS-2 (30ML/each reagent)
(Modified Brown & Brenn)
改良的革蘭氏染色試劑盒（改良的Brown & Brenn）
產品介紹: 革蘭氏染色（Gram Staining）用來鑒別細菌的一種方法，利用細菌細胞壁上的生物化學性質不同，分成革蘭氏陽性（Gram Positive）與革蘭氏陰性（Gram Negative）。用於鑑別革蘭氏陰性和革蘭氏陽性。Gram Stain Kit (MODIFIED BROWN AND BRENN) 應用原理: 細菌的陽性和陰性細胞壁均由肽聚醣(peptidoglycan)組成（革蘭氏陽性細胞壁較厚），並且兩者都將吸收結晶紫(crystal violet)。革蘭氏陰性菌在肽聚醣壁的外部有一層脂多醣(lipopolysaccharide)，在丙酮沖洗液中被破壞，從而使結晶紫(crystal violet)得以區分。這使革蘭氏陰性細菌吸收鹼性品紅染色(basic fuchsin stain)。
革蘭氏陽性細菌：藍色
革蘭氏陰性菌：紅色
其他組織：淡黃色-粉紅色
核：紅色
流程可概括為：染色-脫色-複染。(標準操作請依據原廠網站)
1.如有必要，對切片進行脫蠟(Deparaffinize sections)處理，然後水合成蒸餾水。
2.用龍膽紫溶液(Gentian Violet Solution)覆蓋組織切片，孵育2分鐘。
3.用蒸餾水沖洗玻片，以去除多餘的stain。
4.用Lugol’s Iodine Solution的碘溶液覆蓋組織切片，並孵育1分鐘。
5.在流動的自來水中沖洗滑片，以除去過量的碘(Iodine)。
6.浸入蒸餾水中1次。
7.滴加Gram的脫色劑(Gram’s Decolorizer dropwise)，直到顏色不再滲出為止。
8.在蒸餾水中快速沖洗載玻片（2-5秒）。
9.用番紅O(Safranin O Solution)溶液覆蓋組織切片並孵育4分鐘。
10.在蒸餾水中快速沖洗載玻片以去除多餘的stain。
11.浸入無水酒精一次並吸乾。
12.用Picric Acid – Acetone Solution（0.1％）覆蓋組織切片，並在攪拌下孵育2-10秒。
13.用3種無水酒精(absolute alcohol)迅速脫水。
14.清除2種二甲苯或二甲苯替代物的更換，然後裝入合成樹脂(synthetic resin)中。
Scytek品牌介紹 原廠連結
ScyTek Laboratories成立於1991年，其目標是成為生產生命科學試劑的世界級製造公司。從早期生產ELISA試劑開始，ScyTek逐漸擴大產品線，包括IHC試劑，特殊染色，緩沖液，Mounting Media，固定液，稀釋液和各種客製化試劑等等。從一開始，ScyTek就採用了嚴格的原料篩選流程，以確保所有製造的產品都是最高品質。2011年，ScyTek實驗室獲得了ISO 9001-2008認證，在2016年獲得了ISO 13485認證。
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