乳酸脫氫酶 (Lactate dehydrogenase, LDH) –評估組織和細胞損傷(damage )和毒性(toxicity)。

乳酸脫氫酶 (Lactate dehydrogenase, LDH) 是一種氧化還原酶 (EC 1.1.1.27)，幾乎存在於所有活細胞（動物、植物和原核生物）中。它催化丙酮酸(pyruvate)和乳酸(lactate )的相互轉化，同時伴隨著 NADH 和 NAD+ 的相互轉化。它在缺氧條件(hypoxic conditions)下將糖酵解(glycolysis)的最終產物丙酮酸轉化為乳酸(lactate )。乳酸脫氫酶 (Lactate dehydrogenase, LDH) 定量是有意義的，因為某些乳酸脫氫酶 (Lactate dehydrogenase, LDH) 同工酶的血清水平反映了特定臨床組織中的病理狀況。當組織因疾病、損傷或有毒物質而受損時，細胞 乳酸脫氫酶 (Lactate dehydrogenase, LDH) 被釋放到血液中。由於 乳酸脫氫酶 (Lactate dehydrogenase, LDH) 是一種相當穩定的酶，乳酸脫氫酶 (Lactate dehydrogenase, LDH) 已被廣泛用於評估組織和細胞是否存在損傷(damage )和毒性(toxicity)。

乳酸脫氫酶（LDH）測定試劑盒 | 貨號KTB1110 (1- 20 U/mL)

The kit can detect 1- 20 U/mL of LDH directly in samples

CheKine™乳酸脫氫酶測定試劑盒(Lactate Dehydrogenase Assay Kit, KTB1110) 提供了一種簡單而簡便的比色測定法，用於測量血清，血漿，細胞培養上清液，組織/細胞裂解液培養基，發酵液和其他生物液體中的乳酸脫氫酶(Lactate Dehydrogenase)。 在此比色測定中，LDH將NAD還原為NADH，然後與探針相互作用以產生顏色（λmax= 450 nm）。該測定快速且方便。

原理：為定量樣本中乳酸脫氫酶（LDH）提供了一個簡單便捷的比色法方案。乳酸在乳酸脫氫酶（LDH）的作用下生成丙酮酸，同時使NAD+還原生成NADH，NADH還原WST-8生成橙黃色物質，在450nm處的OD值與LDH成正比。適用樣本：血清、血漿、細胞培養上清、組織/細胞裂解物、發酵液及其它生物液體。

Kit components	• Assay Buffer • Lactate • NAD • WST-8 • Enhancer • Lactate Dehydrogenase Standard (100 U/mL)
Features & Benefits	• Optimized procedure for measuring Lactate Dehydrogenase (LDH) in various samples containing serum, plasma, cell culture supernatants, tissue/cell lysates culture medium, fermentation and other biological fluids. • Simple and easy colorimetric assay. • Quick and convenient.
Calibration range	1- 20 U/mL
Usage notes	• Briefly centrifuge small vials at low speed prior to opening. • Performing several dilutions of your sample to ensure the readings are within the standard value range. • Use fresh samples. If not assayed immediately, samples can be stored at -80°C for one month.
Storage instructions	Storage at –20°C and Keep from light. Stable for at least 6 months at recommended temperature from date of shipment.
Shipping	Gel pack with blue ice.

 

Effects of salinity on SDH and LDH activities in the gill tissue of P. fucata with two shell colors (I: 12h, SDH activity; II: 24h, SDH activity; III: 12h, LDH activity; IV: 24h, LDH activity).

引用文獻

引用文獻 https://www.sciencedirect.com/science/article/pii/S2211383523002800?via%3Dihub#fd2