哺乳細胞 / 膜蛋白抽提試劑盒 | Membrane Protein Extraction Kit貨號K268-50 (ab65400)

代理廠牌：
原廠連結：	http://www.biovision.com
相關下載：	哺乳細胞 / 膜蛋白抽提試劑盒操作手冊

特點

胞器蛋白質分離–系列商品介紹

商品: 哺乳細胞 / 膜蛋白抽提試劑盒Membrane Protein Extraction Kit | 貨號K268-50 (ABCAM ab65400)

Extracts membrane proteins within an hr.

產品介紹: 膜蛋白提取試劑盒 ( Membrane Protein Extraction Kit, K268) 提供優化的緩衝液( buffers) 和試劑 (reagents) ，用於從哺乳動物組織(mammalian tissues)和細胞(cells) 中有效提取膜蛋白。與其他只能提取總細胞膜蛋白（血漿和細胞膜蛋白的組合）的其他可用程序不同，BioVision的試劑盒不僅提取總細胞膜蛋白 ( total cellular membrane proteins ) ，而且還專門純化質膜蛋白 (combinations of plasma and organelle membrane proteins)。該方法提供一致的產量和高純度（超過90％）。使用試劑盒製備的膜蛋白可用於各種應用，如Western印跡 (Western blotting)，2-D凝膠(2-D gels）和酶分析(enzyme analyses) 多種檢測方法。整個過程需要不到1小時。

$cellfractation$

Size	50 assays
SKU+Size	K268-50
Species Reactivity	Mammalian
Applications	Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.
Features & Benefits	• Simple procedure • Fast and convenient • The Membrance Protein Extraction Kit provides an easy and complete protocol for isolating integral membrance proteins efficiently from cultured mammalian cells.
Kit Components	• Homogenize Buffer • Upper Phase Solution • Lower Phase Solution • Protease Inhibitor Cocktail

胞器蛋白分離的應用

探討細胞核內轉錄因子和特定DNA序列相互作用。檢測蛋白質和一段特定DNA序列(label)相互結合的技術原理: 當特定序列的DNA和某蛋白質結合後，整個蛋白質複合體分子量會增加,經由電泳後,在gel中的移動速度變慢,整個band進上可加入特定抗體檢測是否有特定蛋白質Binding( Supershift)
細胞凋亡時,粒線體膜電位去極化的過程會增加膜的通透性並使膜內蛋白,如Cytochrome C, AIF, Smac/DIABLO等分子釋放到細胞質中,引發caspase-9活化, 造成一連串凋亡反應發生. COX4蛋白位於粒腺體細胞內側, 並在Apoptosis發生時依舊保留在粒線體內。
二維電泳與MDLC(液相層析)並列為研究蛋白質體學的重要技術之一。原理: 利用分子量(一維)與等電點(二維)一次可分離大約2000個蛋白質。限制: 膜蛋白(Hydrophobic)在電泳中不易看到.蛋白質若是為low abundant protein則欲得到準確而可信的實驗結果要做胞器分離( sample prefractionation) 胞器分離在2d應用的優點: 移去abundant protein 、增加 low abundant protein、減少蛋白質的複合體發生

產品編號	產品名稱	包裝
K270-50	FractionPREP™ Cell Fractionation Kit 四種胞器萃取一組搞定	50 assays
K256-25 K256-100	Mitochondria/Cytosol Fractionation Kit	25 assays 100 assays
K266-25 K266-100	Nuclear/Cytosol Fractionation Kit	25 assays 100 assays
K267-50	Cytosol/Particulate Separation Kit	50 assays
K269-500	Mammalian Cell Extraction Kit	500 assays
K268-50	Membrane Protein Extraction Kit	50 assays
K288-50	Mitochondria Isolation Kit For Tissue & Cultured Cells	50 assays
K280-50	Mitochondrial DNA Isolation Kit	50 assays
其他相關產品
K808-200	Deproteinizing Sample Preparation Kit	200 assays
K6570-3	EZExtract™ Polyubiquitin Buffer Kit	3 components
K281-50	Genomic DNA Isolation Kit	50 assays
K342-25	HDAC1 Immunoprecipitation (IP) & Activity Assay Kit	25 assays
K341-25	HDAC2 Immunoprecipitation (IP) & Activity Assay Kit	25 assays
K344-25	HDAC3 Immunoprecipitation (IP) & Activity Assay Kit	25 assays
K286-25	Immunoprecipitation (IP) Kit	25 assays
K285-50	Mitochondrial Protein IP Kit	50 assays
K810-1000	Protein Quantitation Kit	1000 assays
K259-50	Yeast Mitochondria Isolation Kit	50 assays
K289-50	Yeast Nuclei Isolation Kit	50 assays

引用文獻

Sun, Yan-Yan et al. (2016) Surface expression of hippocampal NMDA GluN2B receptors regulated by fear conditioning determines its contribution to memory consolidation in adult rats Sci Rep. 2016 Aug 4;6:30743. doi: 10.1038/srep30743.

Sin et al., Acute Treatment of Resveratrol Alleviates Doxorubicin-Induced Myotoxicity in Aged Skeletal Muscle Through SIRT1-Dependent Mechanisms. J Gerontol A Biol Sci Med Sci, Oct 2015; 10.1093/gerona/glv175.

Soto et al., Constitutive and ghrelin-dependent GHSR1a activation impairs CaV2.1 and CaV2.2 currents in hypothalamic neurons. J. Gen. Physiol., Aug 2015; 146: 205 – 219.

Tao et al., Macrophage SR-BI mediates efferocytosis via Src/PI3K/Rac1 signaling and reduces atherosclerotic lesion necrosis. J. Lipid Res., Aug 2015; 56: 1449 – 1460.

Dong et al., GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model. J. Neurosci., Apr 2015; 35: 5409 – 5421.

↑回到頁首↑