一氧化氮合酶(NOS)活性測定試劑盒 | Nitric Oxide Synthase (NOS) Activity Assay貨號K205 (ABCAM, ab211084 Dry Ice)

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特點
Ultrasensitive, Simple, HTP adaptable colorimetric assay kit for NOS activity measurement
產品介紹: 一氧化氮合酶(EC 1.14.13.39)(NOS)是催化L-精氨酸生產一氧化氮(NO)的一類酶。一氧化氮(NO)在神經傳遞,血管調節,免疫應答和凋亡中起重要作用。在NADPH,FAD,FMN,(6R)-5,6,7,8-四氫生物喋呤,鈣調蛋白和血紅素的存在下,NOS用分子氧催化L-精氨酸的胍基氮的五電子氧化,產生NO和L瓜氨酸。 NOS有三種同種型:內皮(eNOS),神經元(nNOS)和誘導型(iNOS)。 nNOS佔中樞神經系統NO的產生,其中NO參與細胞通信和信息存儲。 eNOS在血管中產生NO,並參與血管功能的調節。與其他異構體相反,iNOS在氧化應激條件下從頭顯示,並產生大量的NO作為身體防禦機制的一部分。 BioVision的一氧化氮合酶活性測定試劑盒提供了一種準確和方便的方法來測定各種樣品中的NOS活性。在該測定中,由NOS產生的一氧化氮經歷一系列反應並與Griess試劑1和2反應以產生在540nm具有強吸光度的著色產物。該測定是簡單,敏感和高通量適應性,可以檢測低至5μU的NOS活性。(ABCAM, ab211084 Dry Ice)



| Size | 100 assays |
| SKU+Size | K205-100 (ABCAM, ab211084 Dry Ice) |
| Detection Method | Colorimetry (Abs: 540 nm) |
| Species Reactivity | Mammalian samples |
| Applications | Detection of NOS activity |
| Features & Benefits | • Simple, HTP adaptable protocol Sensitivity as low as 5 µU of NOS activity Colorimetric detection |
| Kit Components | • NOS Assay Buffer • NOS Dilution Buffer • NOS Substrate • NOS Cofactor 1 • NOS Cofactor 2 (25X) • Nitrate Reductase • NOS (Positive Control) • Enhancer • Nitrite Standard • Griess Reagent 1 • Griess Reagent 2 |
| Storage Conditions | -80°C |
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