一氧化氮螢光測定試劑盒| Nitric Oxide Fluorometric Assay Kit貨號K252-200
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特點
Nitric Oxide Fluorometric Assay Kit 一氧化氮螢光測定試劑盒| 貨號K252-200
Detects total nitrate/nitrite
產品介紹: 一氧化氮（NO）在神經傳遞，血管調節，免疫應答和凋亡中起重要作用。 由於NO快速轉化為亞硝酸鹽（NO2ˉ）和硝酸鹽（NOᶟˉ），所以將亞硝酸鹽和硝酸鹽的總濃度用作NO生產的定量測量。 BioVision的一氧化氮熒光測定試劑盒提供了一個簡單的兩步法測定硝酸鹽/亞硝酸鹽的總濃度。 在第一步硝酸還原酶將硝酸鹽轉化成亞硝酸鹽。 在第二步中，亞硝酸鹽與螢光探針DAN（2,3二氨基萘）反應。 NaOH增強螢光產量。 螢光強度與總一氧化氮產量成比例。 試劑盒已經用培養基，血漿和組織勻漿進行了測試。
| Size | 2 x 96 assays |
| SKU+Size | K252-200 |
| Detection Method | Fluorescence (Ex/Em 380/461 nm) |
| Species Reactivity | Mammalian |
| Applications | Measurement of total nitrate/nitrite concentration |
| Features & Benefits | • Simple procedure; takes only 2-3 hours • Fast and convenient • The fluorometric kit provides a simple two-step process for quantitative measurement of total nitrate/nitrite productions. The assay can be easily done in a 96-well plate. |
| Kit Components | • Assay Buffer • Enzyme Cofactor • Enhancer • Nitrate Reductase • Nitrate Standard • Nitrite Standard • DAN Probe • Sodium Hydroxide • Microtiter plate • Plate Cover |
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