產品名稱: Minute™葉綠體分離試劑盒| Detergent-Free Plant Protein Extraction貨號CP-011

Invent Biotechnologies’Minute™ 葉綠體分離試劑盒由優化的葉綠體分離緩衝液和含2.0ml收集管的濾筒組成。該試劑盒旨在從新鮮植物組織（葉，種子和軟莖等）快速提取完整的葉綠體。由於使用具有預定孔徑和厚度的濾芯，可以快速從新鮮植物組織中分離出完整的葉綠體。與需要1-10 + g組織進行葉綠體分離的許多其他方法不同，該試劑盒可以在不到5分鐘的時間內從5-200 mg新鮮植物葉中快速獲得1 X 10⁶至1 X 10⁷完整的葉綠體（> 90％完整）。

產品應用:

MinuteTM葉綠體分離試劑盒旨在旨在快速從新鮮植物組織的小樣本（100-200 mg）中分離出完整的葉綠體，用於生化應用分析，SDS-PAGE，免疫印跡，酶測定等。分離的葉綠體可用作起始原料用於分子生物學研究的純化RNA和DNA的材料。

Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性；用於總蛋白提取，細胞分離和膜蛋白分離，只需簡單四步驟即可完成蛋白質萃取。無須長時間等待、免除重複性的wash步驟。

Item	Amount	Part #
Buffer A (with 5g dissociation beads)	25 ml	CP-011-A
Buffer B	25 ml	CP-011-B
Protein Extraction Filter Cartridges	50	P-001
Collection Tubes with Cap	50	P-002
plastic rods	4	P-003

 

Cat #	Description	Size
SC-003	Cytoplasmic & Nuclear Extraction Kits	50 Preps
SM-005	Plasma Membrane Protein Isolation Kit	50 preps
NE-013	Nuclear Envelope Protein Extraction Kit	50 preps
MP-007	Mitochondria Isolation Kit For Mammalian Cells And Tissues	50 preps
AF-023	Adipose Tissue Fractionation Kit	20 preps
NI-024	Detergent-free nuclei isolation kit	20 preps
YM-017	Yeast Mitochondria Enrichment Kit	50 preps
MM-018	Plant Microsomal Membrane Extraction Kit	50 preps
CP-011	Chloroplast Isolation Kit	50 preps

 

Cat. No	Starting Materials	Status of Extracted Proteins	Yield (mg/ml)	Protocol Time (min)
SD-001/ SN-002	Cultured Animal Cells and Tissues	Denatured/Native	2-8	1-8
SC-003	Mammalian Cultured Cells/Tissues	Native	1-3	6-8
SN-006	Cultured Animal Cells and Tissues	Denatured/Native	1-4	5-8
SM-005	Mammalian Cultured Cells/Tissues	Native Detergent/ EDTA -Free	1-3	20-45
MP-007	Mammalian Cultured Cells/Tissues	Native Detergent/ EDTA-Free	1-3	25-30
SD-008/ SN-009	Fresh Plant Tissues	Denatured/Native	2-8	5-8
SN-010	Fresh Plant Tissues	Native	1-5	6-8
CP-011	Fresh Plant Tissues (5-200 mg/sample)	Chloroplast (>90%) Intact	1-4	5
SB-004	Cultured Bacterial Cells	Denatured	2-3	2-3
NE-013	Cultured cells or Cells from Tissues	Native	1-3	45
HP-014	Cultured cells or Cells from Tissues	Denatured	1-2.5	<10
YT-015	Cultured Microbes	Denatured/Native	2-5	<10 min
YD-016	Cultured Microbes	Denatured/Native	2-4	<10 min
YM-017	Cultured Yeast	Native	2-3	1h
MM-018	Plant tissues	Native	2-3	1h
PN-019	PAGE/Agarose gels	Denatured/Native	NA	10-20 min
HD-021	Keratinized tissues	Denatured	1-2	5 min hand on
AT-022	Adipose Tissues/Adipocytes	Native/Denatured	2-3	20 min
AF-023	Adipose Tissues/Adipocytes	Native	0.5-1	20 min
FE-025	FFPE	Denatured	2-3	1h

 

Sub-Cellular Structure Fractionation/Isolation
Cat #	Description	Size
SC-003	Cytoplasmic & Nuclear Extraction Kits	50 Preps
SM-005	Plasma Membrane Protein Isolation Kit	50 preps
NE-013	Nuclear Envelope Protein Extraction Kit	50 preps
MP-007	Mitochondria Isolation Kit For Mammalian Cells And Tissues	50 preps
AF-023	Adipose Tissue Fractionation Kit	20 preps
NI-024	Detergent-free nuclei isolation kit	20 preps
YM-017	Yeast Mitochondria Enrichment Kit	50 preps
MM-018	Plant Microsomal Membrane Extraction Kit	50 preps
CP-011	Chloroplast Isolation Kit	50 preps