Human Type I Collagen-Solution  | 貨號1200-01S |規格1.0 mL (0.5 mg/mL)

細胞免疫專家極力推薦SouthernBiotech高品質膠原– Type I Collagen

關於膠原（Collagen）

膠原蛋白是動物結締組織重要的蛋白質,在哺乳動物中的含量尤為豐富，佔有機體蛋白質百分之25%-35%含量。膠原是細胞外最重要的水不溶性纖維蛋白, 是構成細胞外基質（extracellular matrix, ECM）的骨架。膠原在細胞外基質中形成半晶體的纖維,給細胞提供抗張力和彈性,並在細胞的遷移和發育中起作用。膠原蛋白的基本結構單位是原膠原(tropocollagen), 原膠原肽鏈的一級結構具有(Gly-x-y)n重複序列, 其中x常為脯氨酸(Pro), y常為羥脯氨酸(Hypro)或羥賴氨酸(Hylys)。Hylys殘基可發生糖基化修飾,其糖單位有的是一個半乳糖殘基(Gal), 但通常是二糖(Glu-Gal-),膠原上的糖所占的量約為膠原的10%。原膠原是由三條α-肽鏈組成的纖維狀蛋白質, 相互擰成三股螺旋狀構型, 長300nm, 直徑1.5nm。目前已發現29種不同組織中不同類型的膠原; 不同類型的膠原定位於體內的特定組織,也有2-3種不同的膠原存在於同一組織中。而機體內90%的膠原為I、II、III、IV型。

Purified Human Type I Collagen (SB Cat. No. 1200-01S) was resolved by electrophoresis, transferred to PVDF membrane, probed with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), and visualized using Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05) secondary antibody and chemiluminescent detection.

 

Description	Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes – fibril (types I, II, III, V) and non-fibril (types IV, VI). Type I collagen is the most abundant collagen and is expressed in almost all connective tissues including skin, tendon, and bone tissue. It is also the predominant component of the interstitial matrix. Type I collagen mutations are associated in a range of diseases including osteogenesis imperfecta and Ehlers-Danlos syndrome. Type I collagen consists of two α1(I) chains and one α2(I) chain.
Source	Placental villi
Purity	> 90% by SDS-PAGE
Purification	Controlled and limited pepsin digestion followed by selective salt precipitation
Alternalte Name(s)	COL1A1, COL1A2
Buffer Formulation	500 mM acetic acid
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C

References

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2. Rizvi MA, Katwa L, Spadone DP, Myers PR. The effects of endothelin-1 on collagen type I and type III synthesis in cultured porcine coronary artery vascular smooth muscle cells. J Mol Cell Cardiol. 1996;28:243-52. (ELISA, Standard Curve)
3. Rizvi MA, Myers PR. Nitric oxide modulates basal and endothelin-induced coronary artery vascular smooth muscle cell proliferation and collagen levels. J Mol Cell Cardiol. 1997;29:1779-89. (ELISA, Standard Curve)
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7. Rosenbloom J, Saitta B, Gaidarova S, Sandorfi N, Rosenbloom JC, Abrams WR, et al. Inhibition of type I collagen gene expression in normal and systemic sclerosis fibroblasts by a specific inhibitor of geranylgeranyl transferase I. Arthritis Rheum. 2000;43:1624-32. (ELISA, Standard Curve)
8. Jimenez SA, Gaidarova S, Saitta B, Sandorfi N, Herrich DJ, Rosenbloom JC, et al. Role of protein kinase C-δ in the regulation of collagen gene expression in scleroderma fibroblasts. J Clin Invest. 2001;108:1395-403. (ELISA, Standard Curve)
9. Howard PS, Renfrow D, Schechter NM, Kucich U. Mast cell chymase is a possible mediator of neurogenic bladder fibrosis. Neurourol Urodyn. 2004;23:374-82. (ELISA, Standard Curve)
10. Absood A, Furutani A, Kawamura T, Graham LM. A comparison of oxidized LDL-induced collagen secretion by graft and aortic SMCs: role of PDGF. Am J Physiol Heart Circ Physiol. 2004;287:H1200-6. (ELISA, Standard Curve)
11. Kottler UB, Jünemann AG, Aigner T, Zenkel M, Rummelt C, Schlötzer-Schrehardt U. Comparative effects of TGF-β1 and TGF-β2 on extracellular matrix production, proliferation, migration, and collagen contraction of human Tenon’s capsule fibroblasts in pseudoexfoliation and primary open-angle glaucoma. Exp Eye Res. 2005;80:121-34. (ELISA, Standard Curve)
12. Dooley A, Gao B, Shi-Wen X, Abraham DJ, Black CM, Jacobs M, et al. Effect of nitric oxide and peroxynitrite on type I collagen synthesis in normal and scleroderma dermal fibroblasts. Free Radic Biol Med. 2007;43:253-64. (ELISA, Standard Curve)
13. Mishra R, Zhu L, Eckert RL, Simonson MS. TGF-β-regulated collagen type I accumulation: role of Src-based signals. Am J Physiol Cell Physiol. 2007;292:C1361-9. (ELISA, Standard Curve)
14. Dooley A, Shi-Wen X, Aden N, Tranah T, Desai N, Denton CP, et al. Modulation of collagen type I, fibronectin and dermal fibroblast function and activity, in systemic sclerosis by the antioxidant epigallocatechin-3-gallate. Rheumatology. 2010;49:2024-36. (ELISA, Standard Curve)
15. Glindtvad C, Chen M, Nygaard JV, Wogensen L, Forman A, Danielsen CC, et al. Electrospun biodegradable microfibers induce new collagen formation in a rat abdominal wall defect model: A possible treatment for pelvic floor repair?. J Biomed Mater Res B Appl Biomater. 2018;106:680-8. (ELISA, Standard Curve)
16. Atamas SP, Luzina IG, Choi J, Tsymbalyuk N, Carbonetti NH, Singh IS, et al. Pulmonary and activation-regulated chemokine stimulates collagen production in lung fibroblasts. Am J Respir Cell Mol Biol. 2003;29:743-9. (WB, Positive Control)
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膠原蛋白

產品名稱	編號（cat#）	包裝形式	規格
Type I Human Collagen	1200-01S	溶液	0.50mg
Type I Bovine Collagen	1200-02/1200-02S	凍乾粉/溶液	0.25mg/0.50mg
Type I Rat Collagen	1200-03/1200-03S	凍乾粉/溶液	0.25mg/0.50mg
Type II Rat Collagen	1220-02/1220-02S	凍乾粉/溶液	0.25mg/0.50mg
Type III Human Collagen	1230-01S	溶液	0.50mg
Type III Bovine Collagen	1240-02S	溶液	0.50mg
Type IV Human Collagen	1250-01S	溶液	0.50mg
Type IV Mouse Collagen	1250-04S	溶液	0.50mg
Type IV Bovine Collagen	1260-02	凍乾粉	0.25mg
Type V Human Collagen	1270-01/1270-01S	凍乾粉/溶液	0.1mg/0.25mg
Type V Bovine Collagen	1280-02S	溶液	0.25mg
Type VI Human Collagen	1290-01S	溶液	0.25mg
Type VI Bovine Collagen	1300-02/1300-02S	凍乾粉/溶液	0.10mg/0.25mg

 

膠原蛋白多株抗體

產品名稱	編號（cat#）	包裝形式	規格
Goat Anti-Type I Collagen	1310-01/1310-08	未標記/生物素化	0.2mg/0.2mg
Goat Anti-Type II Collagen	1320-01/1320-08	未標記/生物素化	0.2mg/0.2mg
Goat Anti-Type III Collagen	1330-01/1330-08	未標記/生物素化	0.2mg/0.2mg
Goat Anti-Type IV Collagen	1340-01/1340-08	未標記/生物素化	0.2mg/0.2mg
Goat Anti-Type V Collagen	1350-01/1350-08	未標記/生物素化	0.2mg/0.2mg
Goat Anti-Type VI Collagen	1360-01/1360-08	未標記/生物素化	0.2mg/0.2mg

膠原蛋白單株抗體

產品名稱	編號（cat#）	包裝形式	規格
Mouse Anti-Human Type I Collagen	1440(1)-01	未標記	0.1mg
Mouse Anti-Human Type IV Collagen	1460-01	未標記	0.1mg
Mouse Anti-Human Type IV Collagen	1460-02	FITC	0.1mg
Mouse Anti-Human Type IV Collagen	1460-08	BIOT	0.1mg
Mouse Anti-Human Type IV Collagen	1460-09	R-PE	0.1mg

應用： ELISA、免疫螢光、免疫細胞化學、免疫組織化學（冰凍切片）

Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |