RNaseOFF核糖核酸酶抑制劑 | Ribonuclease Inhibitor 4,000 U (100 µl) 貨號G138

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商品: RNaseOFF Ribonuclease Inhibitor | 貨號G138 (40 U/μl) 4,000 U (100 µl)
產品介紹: RNaseOFF核糖核酸酶抑制劑 (RNaseOFF Ribonuclease Inhibitor, RNases),特異性抑制核糖核酸酶,包括RNase A核糖核酸酶A( RNase A),RNase核糖核酸酶 B (RNase B)和RNase核糖核酸酶 C(RNase C)。RNaseOFF在PCR或RT-PCR中做為添加劑,安全地保護RNA免受潛在的RNase污染,而不抑制聚合酶活性。具有提高的耐氧化高度氧化敏感度之人類RNase抑制劑(inhibiting polymerase activity)。 RNaseOFF核糖核酸酶抑制劑,即使在非常低濃度的DTT(<1 mM)下也是穩定的,為RNA保護的最佳選擇。可應用於RT-PCR定量、RT-PCR、cDNA合成、體外轉錄、轉譯、RNA分離、製備無RNA酶的抗體、污染敏感等實驗。
RNaseOFF Ribonuclease Inhibitor specifically inhibits common ribonucleases (RNases), including RNase A, B and C, with high affinity. This enzyme is a useful additive in PCR or RT-PCR as it safe-guards RNA against potential RNase contamination without inhibiting polymerase activity. This robust version of RNase inhibitor has improved resistance to oxidation over the highly oxidation-sensitive human RNase inhibitor. RNaseOFF Ribonuclease Inhibitor is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.
一個單位定義: 為將50ng RNA酶A的活性抑制50%所需的RNaseOFF核糖核酸酶抑制劑的量。RNA操件注意事項: 在反應設置期間,始終將RNaseOFF保持在冰上。建議在消除RNase污染的條件下設置所有RNA相關/ RNA酶敏感性工作。移液管吸頭和管應用0.1%DEPC處理。強烈建議在使用RNA時戴手套。
Product Applications:
• RT-PCR.
• Quantitative, real-time RT-PCR.
• In vitro transcription.
• In vitro translation.
• Coupled transcription/translation.
• Protection of mRNA in cDNA synthesis reactions.
• RNA isolation procedures.
• Preparation of RNase-free antibodies
• Eukaryotic RNase contamination sensitive experiment.