商品: RNaseOFF Ribonuclease Inhibitor  | 貨號G138  (40 U/μl)  4,000 U (100 µl)

 產品介紹: RNaseOFF核糖核酸酶抑制劑 (RNaseOFF Ribonuclease Inhibitor, RNases)，特異性抑制核糖核酸酶，包括RNase A核糖核酸酶A( RNase A)，RNase核糖核酸酶 B (RNase B)和RNase核糖核酸酶 C(RNase C)。RNaseOFF在PCR或RT-PCR中做為添加劑，安全地保護RNA免受潛在的RNase污染，而不抑制聚合酶活性。具有提高的耐氧化高度氧化敏感度之人類RNase抑制劑(inhibiting polymerase activity)。 RNaseOFF核糖核酸酶抑制劑，即使在非常低濃度的DTT（<1 mM）下也是穩定的，為RNA保護的最佳選擇。可應用於RT-PCR定量、RT-PCR、cDNA合成、體外轉錄、轉譯、RNA分離、製備無RNA酶的抗體、污染敏感等實驗。

RNaseOFF Ribonuclease Inhibitor specifically inhibits common ribonucleases (RNases), including RNase A, B and C, with high affinity. This enzyme is a useful additive in PCR or RT-PCR as it safe-guards RNA against potential RNase contamination without inhibiting polymerase activity. This robust version of RNase inhibitor has improved resistance to oxidation over the highly oxidation-sensitive human RNase inhibitor. RNaseOFF Ribonuclease Inhibitor is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

一個單位定義: 為將50ng RNA酶A的活性抑制50％所需的RNaseOFF核糖核酸酶抑制劑的量。RNA操件注意事項: 在反應設置期間，始終將RNaseOFF保持在冰上。建議在消除RNase污染的條件下設置所有RNA相關/ RNA酶敏感性工作。移液管吸頭和管應用0.1％DEPC處理。強烈建議在使用RNA時戴手套。

Product Applications:

• RT-PCR.

• Quantitative, real-time RT-PCR.

• In vitro transcription.

• In vitro translation.

• Coupled transcription/translation.

• Protection of mRNA in cDNA synthesis reactions.

• RNA isolation procedures.

• Preparation of RNase-free antibodies

• Eukaryotic RNase contamination sensitive experiment.