人類正常初代視網膜色素上皮細胞 | Human Primary Retinal Pigment Epithelial Cells 貨號T4057
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Abmgood 正常初代細胞系列商品 (Primary cell) |貨號 T4057 5×105 cells / 1.0 ml
人類正常初代視網膜色素上皮細胞 | Human Primary Retinal Pigment Epithelial Cells (貨號T4057)
Human Primary Retinal Pigment Epithelial Cells細胞Adherent / Polygonal (P2第二代) | 來源Retina (Homo sapiens) | T4057。細胞Marker: CK-18, CK-19, fibronectin。細胞培養條件: 使用PriCoat™T25培養瓶 (貨號G299) 以最佳化初代和不朽細胞的生長、增殖和擴增。或塗層細胞外基質(Applied Cell Extracellular Matrix) 增強細胞貼附 (貨號G422)。Grow cells in ECM-coated culture vessels with the following conditions. 培養液Prigrow X series。1%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0°C.
初代細胞 (Primary Cell) 是指直接從活體組織中分離出的細胞，具有有限的細胞壽命無法無限制地繼代培養；與一般學術上常用的細胞株 (Cell Line)不同，具有有限的細胞壽命無法無限制地繼代培養，但卻具有最接近活體狀態的細胞型態與功能特性。其它更多人類初代細胞 胰腺星狀細胞 | 視網膜色素上皮細胞
HOT!! 黴漿菌PCR偵測 | DAPI染核 | 細胞內黴漿菌去除劑 | 水浴槽抑菌劑 | 噴霧式去黴劑 等系列商品PriCoat™ T25 Flasks | type I collagen coating | I 型膠原(type I collagen) | Applied Cell Extracellular Matrix | Prigrow III Medium初代細胞培養液
引用文獻
- Shrivastava, R., Cucuat, N., Rousse, M., Weigand, T., Neto, P., Janicot, C., & Shrivastava, C. “A new generation of topical chronic wound treatments containing specific MMP inhibitors” Chronic Wound Care Management and Research 1:31 (2014).
| Cat. No. | T4057 |
| Name | Human Primary Retinal Pigment Epithelial Cells |
| Organism | Human (H. sapiens) |
| Tissue | Eye |
| Donor History | Normal tissue |
| Growth Properties | Adherent, polygonal |
| Cell Type | Primary Cells |
| Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. |
| Shipping Conditions | Ship with dry ice. |
| Product Format | Frozen |
| Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| BioSafety | II |
| Certificate of Analysis | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. |
| Growth Conditions | Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM4057) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. |
| Unpacking and Storage Instructions |
1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use. To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability. |
| Thawing Protocol |
1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions. |
| Subculture Protocol |
Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent. 1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel. 2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment. 3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel. 4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type. 5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired. 6. Incubate the cells at the recommended conditions. |
| Cryopreservation | Cryopreservation Medium (TM024), or complete growth media with 10% DMSO. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T4057. |