(Uncoated) 膠原蛋白Collagen Type I檢測試劑盒 |貨號SEA571Hu

ELISA Kit for Collagen Type I (COL1）

商品: (Uncoated) 膠原蛋白Collagen Type I檢測試劑盒(酶聯免疫吸附試驗法) |貨號SEA571Hu，偵測的靈敏度62.5-4000pg/mL、最低靈敏度26.8pg/mL。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法)，專門生產研究用檢測試劑的生技公司，於美國研發和生產，產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein；ELISA產品系列超過6000種以上，物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。Uncoated ELISA型式；包括Capture Antibody, Detection Antibody、Standard、Streptavidin-HRP、TMB Substrate, 96-well Plate。

膠原蛋白Collagen Type I檢測試劑盒 (酶聯免疫吸附試驗法)套組內容

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

膠原蛋白Collagen Type I檢測試劑盒簡單流程

1. Prepare all reagents, samples and standards
   2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
   3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
   4. Aspirate and wash 3 times;
   5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
   6. Aspirate and wash 5 times;
   7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C;
   8. Add 50µL Stop Solution. Read at 450nm immediately.

膠原蛋白Collagen Type I檢測相關之抗體 | 蛋白質 | ELISA | CLIA產品

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications
NPA571Hu01	Native Collagen Type I (COL1)	SDS-PAGE; WB; ELISA; IP.
PAA571Hu01	Polyclonal Antibody to Collagen Type I (COL1)	WB, ICC, IHC-P, IHC-F, ELISA
LAA571Hu71	Biotin-Linked Polyclonal Antibody to Collagen Type I (COL1)	IHC;WB;ELISA
SEA571Hu	ELISA Kit for Collagen Type I (COL1)	Enzyme-linked immunosorbent assay for Antigen Detection