Genomic DNA萃取套組 | T-PRO貨號RB94-NGS100

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相關下載：	Genomic DNA萃取套組操作手冊

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Genomic DNA萃取套組 | 貨號RB94-NGS100

T-Pro基因組DNA萃取套件；提供快速和經濟方法純化Total DNA（包括基因組、線粒體和病毒DNA）。可以從全血（新鮮）、血漿、血清、血沉棕黃層、組織、體液、淋巴細胞、細菌和培養細胞萃取genomic DNA。不需要苯酚/氯仿萃取或醇沉澱。純化的DNA適合PCR或其他酶反應。

規格: T-Pro Genomic DNA Mini Kit RB94-NGS100 NT Buffer 45 ml NB Buffer 45 ml W1 Buffer 45 ml W2 Buffer * 25 ml Elution Buffer 30 ml Proteinase K ** 2 x 11 mg GS Columns 100 pcs Collection Tube 100 pcs

Blood Protocol

When the blood sample is less then 50μl or sample consists of nucleated blood cells, the Cultured Cell Protocol is recommended to purify genomic DNA. 1. Add 100 ml ethanol (96-100%) to W2 Buffer prior to the initial use. 2. When the sample is low-copy(<10,000 copies), addition 1µl of aqueous solution Carrier DNA (10µg/µl) to 300µl NB Buffer before use. 3. Additional requirements: Microcentrifuge tube Ethanol (96-100%) RNase A (50 mg/ml) Carrier DNA (e.g., poly dA, poly dA:dT), concentration: 10µg/µl

Cultured Cells Protocol

Cultured Cells Protocol 1. Add 100 ml ethanol (96-100％) to W2 Buffer prior to the initial use. 2. Additional requirements: Microcentrifuge tube Ethano (96-100％) RNase A (50mg/ml) PBS buffer

Bacterial Protocol 1. Add 100 ml ethanol (96-100％) to W2 Buffer prior to the initial use. 2. Additional requirements: Lysozyme Buffer (20mg/ml lysozyme; 20 mM Tris-HCI, 2mM EDTA, 1% Triton X-100, pH 8.0) for Gram-Positive Bacteria Sample. Prepare the lysozyme buffer fresh immediately prior to use. Microcentrifuge tube Ethano (96-100％) RNase A (50mg/ml)

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