Anti-Human IgG Fc-HRP抗體 | Southernbiotech貨號9040-05
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細胞免疫專家極力推薦SouthernBiotech高品質二級抗體
Mouse Anti-Human IgG Fc-HRP抗體 (HRP, conjugated) | 貨號9040-05
美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全，包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質（FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）各種螢光標誌、另具酵素（HRP、AP）、或未標誌及Low Endotoxin/Azide Free（LE/AF）等標定。
ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-HRP (SB Cat. No. 9040-05).
| Clone | JDC-10 |
| Isotype | Mouse IgG1κ |
| Isotype Control | Mouse IgG1-HRP |
| Immunogen | Purified human IgG1 myeloma protein |
| Specificity | Human/Rhesus/Chimpanzee IgG Fc |
| Format/Conjugate | HRP (Horseradish Peroxidase) |
| Buffer Formulation | Supplied in a stock solution in 50% glycerol/50% PBS, pH 7.4; No preservative added |
| Volume | 1.0 mL |
| Storage & Handling | Store at 2-8°C Please refer to product specific (M)SDS |
| Applications |
Applications for relevant formats of this clone include – ELISA – Quality tested 1-15,20 |
| Recommended Dilutions | Please refer to product specific Technical Bulletin |
相關資料Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |Isotype control-免疫球蛋白系列產品 按此
SouthernBiotech antibodies are used in laboratories around the world, accelerating the advancement of SARS-CoV-2 serological assays, vaccine development, and COVID-19 research by our global network of collaborators. Our in-house manufacturing capabilities ensure long-term viability of assays and products once released to the market.
Summary of immunoglobulin classes and subclasses –
Immunoglobulin Classes – IgG, IgM, IgA, IgD, IgE
Mouse Immunoglobulin Subclasses – IgG1, IgG2a/IgG2c, IgG2b, IgG3
Human Immunoglobulin Subclasses – IgG1, IgG2, IgG3, IgG4, IgA1, IgA2
Rat Immunoglobulin Subclasses – IgG1, IgG2a, IgG2b, IgG2c
Hamster Immunoglobulin Subclasses – IgG1, IgG2, IgG3
Light Chains – Kappa, Lambda
Heavy Chains – IgG (gamma/γ), IgM (mu/μ), IgA (alpha/α), IgD (delta/δ), IgE (epsilon/ε)
| Cat. No. | Product Description | Clone | Size |
| 2053-01 | Goat Anti-Human IgA, Mouse/Bovine/Horse SP ads-UNLB | Polyclonal | 1.0 mg |
| 9020-05 | Mouse Anti-Human IgM-HRP | SA-DA4 | 1.0 mL |
| 9020-01 | Mouse Anti-Human IgM-UNLB | SA-DA4 | 0.5 mg |
| 9130-05 | Mouse Anti-Human IgA1-HRP | B3506B4 | 1.0 mL |
| 9130-01 | Mouse Anti-Human IgA1-UNLB | B3506B4 | 0.5 mg |
| 9140-05 | Mouse Anti-Human IgA2-HRP | A9604D2 | 1.0 mL |
| 9140-01 | Mouse Anti-Human IgA2-UNLB | A9604D2 | 0.5 mg |
| 2014-05 | Goat Anti-Human IgG Fc, Multi-Species SP ads-HRP | Polyclonal | 1.0 mL |
| 2014-01 | Goat Anti-Human IgG Fc, Multi-Species SP ads-UNLB | Polyclonal | 0.5 mg |
| 9040-05 | Mouse Anti-Human IgG Fc-HRP | JDC-10 | 1.0 mL |
| 9040-01 | Mouse Anti-Human IgG Fc-UNLB | JDC-10 | 0.5 mg |
| 9042-05 | Mouse Anti-Human IgG Fc-HRP | H2 | 1.0 mL |
| 9042-01 | Mouse Anti-Human IgG Fc-UNLB | H2 | 0.5 mg |
Conjugates偶聯物的選擇取決於應用和檢測技術（比色法、化學發光法或螢光法）。 對於蛋白質印跡和 ELISA 應用，通常使用酶偶聯二抗，例如辣根過氧化物酶 (HRP) 和鹼性磷酸酶 (AP)，並且都可以使用比色和化學發光平台進行檢測。 對於流式細胞術或免疫螢應用，需要與螢染料偶聯的二抗（例如，Alexa Fluor®、FITC、TRITC、APC、PE）。
| Cat. No. | Product Description | Clone | Size |
| 2053-01 | Goat Anti-Human IgA, Mouse/Bovine/Horse SP ads-UNLB | Polyclonal | 1.0 mg |
| 9020-05 | Mouse Anti-Human IgM-HRP | SA-DA4 | 1.0 mL |
| 9020-01 | Mouse Anti-Human IgM-UNLB | SA-DA4 | 0.5 mg |
| 9130-05 | Mouse Anti-Human IgA1-HRP | B3506B4 | 1.0 mL |
| 9130-01 | Mouse Anti-Human IgA1-UNLB | B3506B4 | 0.5 mg |
| 9140-05 | Mouse Anti-Human IgA2-HRP | A9604D2 | 1.0 mL |
| 9140-01 | Mouse Anti-Human IgA2-UNLB | A9604D2 | 0.5 mg |
| 2014-05 | Goat Anti-Human IgG Fc, Multi-Species SP ads-HRP | Polyclonal | 1.0 mL |
| 2014-01 | Goat Anti-Human IgG Fc, Multi-Species SP ads-UNLB | Polyclonal | 0.5 mg |
| 9040-05 | Mouse Anti-Human IgG Fc-HRP | JDC-10 | 1.0 mL |
| 9040-01 | Mouse Anti-Human IgG Fc-UNLB | JDC-10 | 0.5 mg |
| 9042-05 | Mouse Anti-Human IgG Fc-HRP | H2 | 1.0 mL |
| 9042-01 | Mouse Anti-Human IgG Fc-UNLB | H2 | 0.5 mg |
Max D. Cooper
Emory University School of Medicine
美國抗體生產商Southern Biotech於1982年，由阿拉巴馬大學伯明翰分校 UAB拉斯克獎得主細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。在過去的 37 年中，SouthernBiotech 已將 Cooper 實驗室產生的眾多雜交瘤商業化，包括幾種禽類特異性抗體，其模型有助於開發適應性免疫的核心原理。致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。
二級抗體 (Secondary antibodies) 應用:
二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。