ATPase分析套組 |Innova biosciences貨號601-0120 (含PiColorLock） (abcam ab270551)
Colorimetric ATPase分析套組 |貨號601-0120（含PiColorLock） (abcam ab270551)
601-0120 High Throughput Colorimetric ATPase Assays
Innova Biosciences 提供之ATPase分析套組，可探討酵素活性是否扮演transport、signal transduction、protein biosynthesis及Cell differentiation之角色。應用於ATPase活性分析，提供high-throughput screen 大量ATPase inhibitor。可建立快速藥物篩選系統。採用PiColorLock™超高靈敏度Malachite green磷酸根呈色方法，分析ATPase酵素活性，克服傳統呈色方法、低靈敏度限制。ATPase酵素分析套組，包括stabilizing solution，可避開acid labile substrate所造成的高背景值，其背景值很低（1mM）。其分析波段為600－660nm，避開一般colored compounds之干擾。Pi-dye複合物極為穩定，其訊號強度可維持數小時之久。適用於大部份的assay buffer。蛋白質濃度高時，不會抑制顏色的形成。
PiColorLock™磷酸根檢測試劑，為檢測無機磷酸根（Pi）之含量；使用Malachite green磷酸根呈色法。可用於測量產生Pi的任何酶，包括ATPases、GTPases, phosphatases、熱休克蛋白及DNA解旋蛋白。PiColorLock™的獨特配方提高了測定線性，動態範圍和顏色穩定性；也可以用PiColorLock與不穩定的底物（例如ATP，GTP）一起使用，所述底物與其它酸性染料基檢測試劑產生高的非酶背景信號。此產品不具放射性應用Malachite green磷酸根呈色法，為高靈敏的磷酸根偵測試劑，確保穩定的綠色dye-phosphate複合物產生，避免發生受質非酵素水解干擾造成的背景雜訊。偵測範圍廣，可減少樣品稀釋的必要。
| Features | Benefits |
| Colorimetric assay | Simple, scalable and non-radioactive |
| Compatible with almost any assay buffer | Flexibility over a wide range of existing assays |
| Stable reagent formulation | Long shelf life |
| Unique accelerator | Speeds up color development |
| Unique stabilizer | Suppresses non enzymatic backgrounds with acid-labile substrates (ATP or GTP) |
| PiColorLock™-Pi complex is very stable | No precipitation, results can be measured over several hours – ideal for HTS |
| Wide linear range | No inhibition of color development by high concentrations of protein |
ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ provides baseline readings.
Dynamic Range for ATPase Assay Kit (ab270551).
PiColorLockTM has been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.
| 產品型號 | 產品名稱 | 包裝 |
| 303-0125 303-0500 |
PiColorLock Gold Phosphate Detection System（靈敏度4－40uM） | 最多1560 assays 最多6250 assays |
| 601-0120 601-0121 601-0122 |
High Throughput Colorimetric ATPase Assays | 192 assays 480 assays 5 × 480 assays |
| 關聯商品 | ||
| 601-9999 | 10mM Lyophilized ATP | 0.5ml |
| 602-0120 602-0121 602-0122 |
High Throughput Colorimetric GTPase Assays | 192 assays 480 assays 5 × 480 assays |
| 501-0015 | PiBind resin－快速移除樣品中污染的磷酸根（Pi） | 5g |