呈色螢光分析!!   ATP呈色分析套組ATP Colorimetric/Fluorometric Assay Kit | 貨號K354-100 Abcam ab83355

產品介紹: Biovision 提供一系列ADP/ATP冷光及化學呈色分析套組；其中ATP呈色螢光分析套組；其利用甘油的磷酸化產生，可以呈色（λmax = 570nm）或螢光測定（Ex / Em = 535/587 nm）兩種方法測量ATP之含量。

ATP是生命系統的主要能量。ATP僅在粒線體中形成，多種遺傳疾病可影響線粒體中的ATP形成。ATP測定（例如BioVision Kit 254-200）檢測飛秒以下的ATP，這些試劑盒需要具有分注器之冷光儀器。BioVision新開發的ATP比色和螢光測定試劑盒設計，提供簡單的方法，以呈色（λmax = 570nm）或螢光測定（Ex / Em = 535/587 nm）方法。 該測定在各種樣品中檢測低至50 picomolμM的ATP含量。

Maiti AK et al (2018) used ATP assay kit ab83355 to measure mitochondrial ATP generation in an in vitro mouse intestinal model treated with cytokines in the presence and absence of VIP (vasoactive intestinal peptide). VIP was induced by C. rodentium infection and cytokines.

he chart shows a comparison of ATP levels of HepG2 treated with 0, 10 and 100 µM for 48 hours, DR^{H2O2 W1} (damage recovered cells using hydrogen peroxide with a recovery time of one week) HepG2 cells and MDA-MB-231 cells treated with 0, 10 and 100 µM of NaHS for 48 hours. Data is shown as percent of ATP levels in untreated cells. ATP levels were determined using ATP assay kit (ab83355).

MCF-7 cells are transfected with vector, osteopontin-a, osteopontin-c or osteopontin -a plus -c. Cells are plated on poly-HEMA and seeded at 4 x 10⁵ cells per well and incubated for two days under standard culture conditions. ATP levels are measured using ATP assay kit (ab83355)

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ab83355 has been referenced in 198 publications.