Rat腫瘤壞死因數α(TNFα)檢測試劑盒 |Cloud-Clone corp貨號SEA133Ra
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腫瘤壞死因數α(TNFα)檢測試劑盒(酶聯免疫吸附試驗法) |貨號SEA133Ra
ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)
商品: 腫瘤壞死因數α(TNFα)檢測試劑盒|貨號SEA133Ra,偵測的靈敏度15.6-1,000pg/mL、最低靈敏度6.1pg/mL。ELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法),專門生產研究用檢測試劑的生技公司,於美國研發和生產,產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein;ELISA產品系列超過6000種以上,物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。
腫瘤壞死因數α(TNFα)檢測試劑盒套組內容
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
免疫分析法的概括流程
- Prepare all reagents, samples and standards;
- Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
- Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
- Aspirate and wash 3 times;
- Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
- Aspirate and wash 5 times;
- Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C;
- Add 50µL Stop Solution. Read at 450nm immediately.
腫瘤壞死因數α(TNFα)檢測試劑盒套組內容
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications |
APA133Ra01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays; In vivo assays. |
RPA133Ra01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | SDS-PAGE; WB; ELISA; IP. |
PAA133Ra01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. |
LAA133Ra81 | FITC-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | IHC;ICC;IF;WB |
LAA133Ra71 | Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | IHC;WB;ELISA |
MAA133Ra21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. |
SEA133Ra | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection |
SCA133Ra | CLIA Kit for Tumor Necrosis Factor Alpha (TNFa) | Chemiluminescent immunoassay for Antigen Detection |