不朽小膠質細胞-SV40 Large T | Human Microglia – SV40貨號 T0251
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Abmgood不朽系列商品 (immortalized cell)-Immortalized Human Microglia
Abmgood不朽化系列商品 (immortalized cell) |貨號 T0251 1×106 cells / 1.0 ml
不朽小膠質細胞 |貨號T0251 |包裝1×106 cells / 1.0 ml Immortalized Human Microglia – SV40
小膠質細胞(Microglia cells)是大腦和脊髓的常駐巨噬細胞，它們是神經系統中主動免疫防禦的第一種也是主要形式。永生化人小膠質細胞係來源於原代人小膠質細胞（>99% 純度），並保留了小膠質細胞標記物 CD68 和 NGF。這些細胞適用於研究健康和疾病中的人類小膠質細胞( human microglia )。
小膠質細胞Immortalized Human Microglia細胞株 | Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen | 來源Brain 。Microglia cells小膠質細胞是大腦和脊髓巨噬細胞，在神經系統中主要的免疫防禦形式。不朽之人小膠質細胞 – SV40細胞株，源自初代人類小神經膠質細胞（> 99％純度），並且通過RT-PCR證實保留小膠質細胞特異性標記物；例如NGF，CD68和TREM2。Expression Profile: NGF, Iba1, TREM2, CD11b, CD68, Sall1, CD45。這些細胞提供人類小膠質細胞的健康和疾病之研究模式。
Microglia cells小膠質細胞培養條件 Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available in ABM, Cat. No.TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and 1% Penicillin/Streptomycin (G255). This cell line needs to be cultured in collagen coated flasks. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0°C.
必需購買PriCoat™T25培養瓶 (貨號G299)，培養液PriGrow III (TM003)。
Abmgood不朽化系列商品 (immortalized cell)
根據細胞類型，Primary cells初代細胞在體外具有小於10-50次傳代的有限壽命。 不朽化細胞株能夠延長增殖，並且具有與親代組織相似或相同的基因型和表現型。
哺乳動物細胞的不朽化Immortalized策略
存在用於在培養物中不朽化哺乳動物細胞的幾種方法。 Abmgood多年研發經驗，開發出了一系列Immortalized商品；包括即用型逆轉錄病毒，慢病毒和腺病毒載體。
Abmgood不朽化系列商品 (immortalized reagenet) Ready-to-use
Abmgood公司於人類與老鼠不朽之細胞株上，具有幾十年的實驗經驗；提供一系列不朽 (immortalized)化之細胞株、及不朽化之試劑商品。ready-to-use定量好之retrovirus、lentiviru、 adenovirus，可直接加於細胞中進行感染表現；病毒液包括有SV40重組病毒、EBV重組病毒、hTERT病毒液、Myc基因病毒液、p53基因病毒液、Rb基因病毒液、Ras基因病毒液。提供106及109兩種包裝力價之病毒液；亦提供一系列病毒力價濃縮與定量試劑商品。
細胞不朽化的工具中，SV40 T antigen是最簡單且最常用來造成不朽化細胞生成的方法。多數的病毒基因和抑癌基因（p53、Rb等）的不活化相關，會誘導細胞處於replicative senescence的階段。目前也有研究顯示被感染的細胞，SV40 T anbtigen會誘導端粒酶（Telomerase）的活性。與端粒（telomere）長度相關的Telomerase Reverse Transcriptase protein (TERT)是一種不朽細胞的研究應用。平常細胞中，hTERT是處於不活化態，必要時會進行活化保持telomere的長度，避免細胞發生replicative senescence的現象。某些細胞hTERT 過度表現（over-expression）對於誘發細胞不朽化是無效的（如初代上皮細胞），反而會造成細胞死亡；同時表現hTERT catalytic subunit以及p53或RB siRNA在人類初代卵巢上皮細胞會造成細胞不朽化。過度表現Ras或Myc T58A mutants也有初代細胞不朽化的現象產生。
ABM提供以下不朽細胞的研究工具：
- Gene Immortalization (Myc, p53, Rb, Ras) Reagents
- HPV E6-E7 Cell Immortalization Reagents
- SV40 T antigen Cell Immortalization Reagents
- EBV Cell Immortalization Reagents
- hTERT Cell Immortalization Reagents
SV40 Cell Immortalization Reagents不杤化系列商品
HPV Cell Immortalization Reagents不杤化系列商品
| Lenti-HPV-16 E6/E7 Virus | pLenti-III-HPV-16 E6/E7 | Lentivirus containing HPV-16 E6/E7 gene (106 IU/ml) | 10ml | G268 |
| Lenti-HPV-16 E6/E7 Virus, High Titer | pLenti-III-HPV-16 E6/E7 | High Titer Lentivirus containing HPV-16 E6/E7 gene (109IU/ml) | 5 x 20 μl | LV617 |
EBV Cell Immortalization Reagents不杤化系列商品
| Product |
Vector Map (Click to View) |
Description | Quantity | Cat. No. |
| EBV Virus | N/A | Virus containing EBV gene (106 IU/ml) | 10ml | G229 |
hTERT Cell Immortalization Reagents不杤化系列商品
| Product |
Vector Map (Click to View) |
Description | Quantity | Cat. No. |
| Adeno-hTERT | pAdeno-hTERT | Adenovirus containing hTERT (106 IU/ml) | 250 μl | G205 |
| Adeno-hTERT Antisense | pAdeno-hTERT Antisense | Adenovirus containing antisense hTERT (106 IU/ml) | 250 μl | G208 |
| Lenti-CMV-hTERT-GFP-2A-Puro Virus | pLenti-CMV-hTERT-GFP-2A-Puro | Lentivirus containing hTERT with a GFP Reporter & resistance to Puromycin (106 IU/ml) | 10ml | LV623 |
| Lenti-CMV-hTERT-GFP-2A-Puro Virus, High Titer | pLenti-CMV-hTERT-GFP-2A-Puro | High Titer Lentivirus containing hTERT with a GFP Reporter & resistance to Puromycin (109 IU/ml) | 5 x 20 μl | LV624 |
| Lenti-CMV-hTERT-RFP-2A-Puro Virus | pLenti-CMV-hTERT-RFP-2A-Puro | Lentivirus containing hTERT with a RFP Reporter & resistance to Puromycin (106 IU/ml) | 10ml | LV625 |
| Lenti-CMV-hTERT-RFP-2A-Puro Virus, High Titer | pLenti-CMV-hTERT-RFP-2A-Puro | High Titer Lentivirus containing hTERT with a RFP Reporter & resistance to Puromycin (109 IU/ml) | 5 x 20 μl | LV626 |
| Lenti-EF1α-hTERT Virus | pLenti-EF1a-hTERT | Lentivirus containing hTERT (106 IU/ml) | 10ml | G706 |
| Lenti-EF1α-hTERT Virus, High Titer | pLenti-EF1a-hTERT | High Titer Lentivirus containing hTERT (109 IU/ml) | 5 x 20 μl | LV616 |
| Lenti-hTERT virus | pLenti-hTERT | Lentivirus containing hTERT (106 IU/ml) | 10ml | G200 |
| Lenti-hTERT Virus, High Titer | pLenti-hTERT | High Titer Lentivirus containing hTERT (109 IU/ml) | 5 x 20 μl | LV615 |
| Lenti-hTERT Antisense virus | pLenti-hTERT Antisense | Lentivirus containing antisense hTERT (106 IU/ml) | 10ml | G201 |
| Lenti-hTERT-Neo Virus | pLenti-hTERT-Neo | Lentivirus containing hTERT with resistance to Neomycin (106 IU/ml) | 10ml | G204 |
| Lenti-hTERT-Neo Virus, High Titer | pLenti-hTERT-Neo | High Titer Lentivirus containing hTERT with resistance to Neomycin (109 IU/ml) | 5 x 20 μl | LV622 |
| Retro-E1/hTERT virus | pRetro-E1 hTERT | Retrovirus containing hTERT (106 IU/ml) | 10ml | G207 |
Gene Immortalization (Myc, p53, Rb, Ras, CDK4, BmiI) Reagents不杤化系列商品
| Product |
Vector Map (Click to View) |
Description | Quantity | Cat. No. |
| Lenti-p53 siRNA Virus | Lenti-p53 siRNA | Recombinant p53 siRNA Lentivirus (106 IU/ml) | 10ml | G219 |
| Lenti-p53 siRNA Virus, High Titer | Lenti-p53 siRNA | High Titer Recombinant p53 siRNA Lentivirus (109 IU/ml) | 5 x 20 μl | LV619 |
| Lenti-Rb siRNA Virus | Lenti-Rb siRNA | Recombinant Rb siRNA Lentivirus (106 IU/ml) | 10ml | G223 |
| Lenti-Rb siRNA Virus, High Titer | Lenti-Rb siRNA | High Titer Recombinant Rb siRNA Lentivirus (109 IU/ml) | 5 x 20 μl | LV621 |
| Lenti-Bmi1 Virus, High Titer | pLenti-Bmi1 | High Titer Recombinant Bmi1 Lentivirus (109 IU/ml) | 5 x 20 μl | LV608 |
| Lenti-Bmi1 Virus | pLenti-Bmi1 | Recombinant Bmi1 Lentivirus (106 IU/ml) | 10ml | LV610 |
| Lenti-CDK4 Virus, High Titer | pLenti-CDK4 | High Titer Recombinant CDK4 Lentivirus (109 IU/ml) | 5 x 20 μl | LV609 |
| Lenti-CDK4 Virus | pLenti-CDK4 | Recombinant CDK4 Lentivirus (106 IU/ml) | 10ml | LV611 |
| Lenti-Myc T58A Virus | pLenti-Myc T58A | Recombinant Myc T58A Lentivirus (106 IU/ml) | 10ml | G217 |
| Lenti-Myc T58A Virus, High Titer | pLenti-Myc T58A | High Titer Recombinant Myc T58A Lentivirus (109 IU/ml) | 5 x 20 μl | LV618 |
| Lenti-Ras V12 Virus | pLenti-Ras V12 | Recombinant Ras V12 Lentivirus (106 IU/ml) | 10ml | G221 |
| Lenti-Ras V12 Virus, High Titer | pLenti-Ras V12 | High Titer Recombinant Ras V12 Lentivirus (109 IU/ml) | 5 x 20 μl | LV620 |
