• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

• 37°C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 ml tubes
• Distilled water
• Absorbent filter papers
• 100 ml and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

• 1) Set standard, test samples and control wells.

• 2) Aliquot 100 µl of diluted standard into the standard wells.

• 3) Aliquot 100 µl of Standard Diluent buffer into control (zero) well.

• 4) Aliquot 100 µl of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.

• 5) Aliquot 100 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.

• 6) Wash 3 times.

• 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 90 mins at 37 °C.

• 8) Wash 5 times.

• 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.

• 10) Aliquot 50 µl of Stop Solution.

• 11) Measure the OD at 450 nm.

Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Troponin I, Cardiac Muscle (TNNI3) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Troponin I, Cardiac Muscle (TNNI3) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) × 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

This product is for research use only.

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.