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豬表皮生長因子 (EGF) ELISA 試劑盒 | 貨號CK-bio-17641

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豬表皮生長因子-EGF操作手冊

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豬表皮生長因子 (EGF) ELISA 試劑盒 | 貨號CK-bio-17641

Porcine Epidermal growth factor (EGF) ELISA Kit

偵測範圍: 1pg/mL -200pg/mL , 最低靈敏度 1.0pg/mL

Double speed Elisa 試劑盒在 90 分鐘~120分鐘即可快速獲得實驗結果; 保持著卓越的偵測專一性與靈敏度

 

For samples:Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

產品描述:該試劑盒用於檢測豬表皮生長因子 (EGF)水平,基於雙重原理(sandwich technology)抗體夾心技術酶聯免疫吸附試驗(enzyme linked immunosorbent assay , ELISA)。將標準品(Standard)和Sample 加入到預先包被有目標抗體的孔中,然後加入HRP-Conjugate 試劑形成免疫複合物,孵育,通過孵育和洗滌,去除未結合的酶,然後加入底物A和B,溶液會轉藍色,最後在酸的作用下變成黃色。顏色深度或光是與豬表皮生長因子 (EGF)濃度呈正相關。偵測範圍: 1pg/mL -200pg/mL , 最低靈敏度 1.0pg/mL。可應用於以下樣品來源:血清(Serum)、血漿(plasma)、尿液(urine)、唾液(saliva)、組織勻漿(tissue homogenates)、細胞裂解物(cell lysates)、細胞培養物上清液(cell culture supernates)和其他生物體(biological fluids)液。

豬表皮生長因子 (EGF)ELISA 試劑盒

Name Porcine Epidermal growth factor (EGF) ELISA Kit
Cat No. CK-bio-17641
Specification 48T/96T/5*96T/10*96T
Detection range 1pg/mL -200pg/mL
Detection limit  1.0pg/mL
Test principle Quantitative
Test method Sandwich
Storage The unopened kit should be stored at 2 – 8°C. After opening, please store refer to the manual.
Sample type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate.

網站規格小圖_工作區域 1_工作區域 1

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CK-bio-17633 Porcine Endothelial nitric oxide synthase,eNOS ELISA Kit ELISA
CK-bio-17634 Porcine endothelin 1,ET-1 ELISA Kit ELISA
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CK-bio-17636 Porcine Endothelin-1 receptor,EDNRA ELISA Kit ELISA
CK-bio-17637 Porcine epidemic diarrhea virus antibody,PEDV ELISA Kit ELISA
CK-bio-17638 Porcine epidermal Fatty acid binding protein,E-FABP ELISA Kit ELISA
CK-bio-17639 Porcine Epidermal growth factor receptor 2,HER2 ELISA Kit ELISA
CK-bio-17640 Porcine epidermal growth factor receptor,EGFR ELISA Kit ELISA
CK-bio-17641 Porcine Epidermal growth factor,EGF ELISA Kit ELISA
CK-bio-17642 Porcine epidermal keratin,EK ELISA Kit ELISA
CK-bio-17643 Porcine epiligrin,EP ELISA Kit ELISA
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操作步驟:

從室溫平衡20-30min後的鋁箔袋中取出所需板條,剩餘板條用自封袋密封放回4℃。

1.標準品的加樣:設置標準品孔和樣本孔,標準品孔各加不同濃度的標準品50μL。

2.加樣:分別設空白孔(空白對照孔不加樣品及酶標試劑,其餘各步操作相同)、待測樣品孔。在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然後再加待測樣品10μl(樣品最終稀釋度為5倍)。加樣將樣品加於酶標板孔底部,儘量不觸及孔壁,輕輕晃動混勻。

3.加酶:每孔加入酶標試劑100μl,空白孔除外。

4.溫育:用封板膜封板後置37℃溫育60分鐘。

5.配液:將20倍濃縮洗滌液用蒸餾水20倍稀釋後備用。

6.洗滌:小心揭掉封板膜,棄去液體,甩幹,每孔加滿洗滌液,靜置30秒後棄去,如此重複5次。

7.顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震盪混勻,37℃避光顯色15分鐘

8.終止:每孔加終止液50μl,終止反應(此時藍色立轉黃色)。

9.測定:以空白孔調零,450nm波長依序測量各孔的吸光度(OD值)。 測定應在加終止液後15分鐘以內進行。

網站規格小圖 品牌介紹_工作區域 1

Coon-koon台灣代理商-BRAND/AGENCY/代理品牌-Coon-koon

http://www.coonkoonbio.com/

Shanghai Coon Koon Biotech Co.,Ltd是一家專業從事生物製品研發、生產和經營的綜合性生物公司! 產品遠銷多個國家Coon Koon Bio主要做ELISA試劑盒、生化試劑、抗體等上萬種科研產品!

涉及分子生物學、細胞生物學、免疫學等各個領域!堅持“以質量為前提,以客戶滿意為宗旨”;確立“以客戶需求為導向,以市場需求為導向,不斷開發優質新產品,提供客戶滿意。

網站規格小圖 相關試劑_工作區域 1

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