GST Colorimetric Activity Assay Kit呈色分析套組 |貨號K263-100 (ABCAM ab65326) | 規格100個分析

產品描述: Biovision提供一系列氧化相關分析套組；其中GST呈色分析套組，以吸收光Absorbance (380 nm )偵測；可用於細胞組織lysates、懸浮培養液、尿液、血漿、或血清之多種液體型式的樣品來源。操作時間大約60-90分鐘。 GST檢測最低靈敏度：活性< 4 mU/well

GST Colorimetric Activity Assay Kit | Abcam

穀胱甘肽S-轉移酶（Glutathione S-transferase , GST）是在異種生物的解毒扮演重要角色。GST催化穀胱甘肽(glutathione)的硫醇基團(thiol group)形成親電子異物(electrophilic xenobiotics)。GST利用穀胱甘肽(glutathione)清除潛在的有毒化合物，包括由於oxidative stress而產生的化合物，並且是防止這些化合物致突變，致癌和毒性作用的防禦機制。 GST呈色活性測定試劑盒，其原理基於GST-催化的GSH和GST底物CDNB（1-氯-2,4-二硝基苯，其具有最寬範圍的同工酶可檢測性（例如，α-，mu-，pi-, and other GST isoforms)。在某些條件下，穀胱甘肽和CDNB之間的相互作用完全取決於活性GST的存在，GST催化的GS-DNB的形成產生二硝基苯基硫醚，其可以通過分光光度計在340 nm。一個單位的GST活性 (One unit of GST activity) 定義為: 在測定條件下產生1μmolGS-DNB綴合物的酶的量，該試劑盒可以檢測粗細胞裂解物或純化的蛋白質級分中的GST活性， GST檢測最低靈敏度：活性GST <4 mU。

GST呈色分析套組 | K263-100內容物 (ABCAM ab65326)
• GST Assay Buffer
• GST Substrate (CDNB)
• Glutathione (GSH, lyophilized)
• GST Positive Control

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