總膽固醇和膽固醇酯比色測定試劑盒II | Abcam貨號ab102515
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總膽固醇和膽固醇酯比色測定試劑盒II | Abcam貨號ab102515
Cholesterol/Cholesteryl Ester Assay Kit – Detection (ab102515)
膽固醇 (Cholesterol)是所有動物生命中必不可少的分子。它涉及正常發育(normal development)和疾病(diseases)。膽固醇/膽固醇酯定量試劑盒 (Cholesterol/Cholesteryl Ester Quantitation Kit) 提供了一種使用比色法靈敏定量游離膽固醇(free cholestero)，膽固醇酯(cholesteryl esters)或兩者的簡單方法。血液中的大多數膽固醇是膽固醇酯(cholesteryl esters)的形式。這些酯(esters )可在適當條件下水解成游離膽固醇(free cholesterol)。在該測定中，游離膽固醇(free cholesterol)被膽固醇脫氫酶(cholesterol dehydrogenase)氧化以產生NADH，其與敏感探針反應，導致450nm處的強吸收。該測定可以檢測游離膽固醇(free cholesterol)或總膽固醇(total cholesterol)，這取決於酯酶是否用於水解存在的膽固醇酯。膽固醇酯 ( Cholesteryl ester )可以通過從總膽固醇（膽固醇加膽固醇酯）中減去游離膽固醇(free cholesterol )的值來確定。該試劑盒中的探針更穩定，靈敏且特異。該測定可以顯著地耐受來自各種樣品的干擾。
| Cat # +Size | K623-100 (ab102515) |
|---|---|
| Size | 100 assays |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Mammalian |
| Applications | N/A |
| Features & Benefits | • Simple procedure; takes ~1 hour • Fast and convenient • Kit contains the necessary reagents for measurement of cholesterol in various biological samples |
| Kit Components | • Cholesterol Assay Buffer • Substrate Mix (lyophilized) • Enzyme Mix (lyophilized) • Esterase (lyophilized) • Cholesterol Standard (2 µg/µl) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
引用文獻
Sundaram, M. et al., (2017) he apolipoprotein C-III (Gln38Lys) variant associated with human hypertriglyceridemia is a gain-of-function mutation, Journal of Lipid Research, Sep.2017, jlr-M077313
Lin, NN et al., (2017) Bacillus Subtilis-Fermented Red Bean (Red Bean Natto) Reduces Hyperlipidemia Levels in Hamsters Fed an Atherogenic Diet, J Food Biochemistry, 2017, 41(1): online
Liu et al., Apoc2 loss-of-function zebrafish mutant as a genetic model of hyperlipidemia. Dis. Model. Mech., Aug 2015; 8: 989 – 998.
Xu et al., Rutaecarpine suppresses atherosclerosis in ApoE–/– mice through upregulating ABCA1 and SR-BI within RCT. J. Lipid Res., Aug 2014; 55: 1634 – 1647.
Watanabe et al., Lipocalin 2 binds to membrane phosphatidylethanolamine to induce lipid raft movement in a PKA-dependent manner and modulates sperm maturation. Development, May 2014; 141: 2157 – 2164.
https://www.abcam.com/cholesterolcholesteryl-ester-assay-kit-detection-ab102515.html#description_references
功能研究 – 膽固醇/膽固醇酯檢測試劑盒 (ab102515)
膽固醇標準曲線：根據試劑盒說明對膽固醇/膽固醇酯進行定量。已從所有讀數中減去 0 標準讀數（不含膽固醇）的背景。