NOVA Histo (20X) 三合一脫石蠟、再水化(rehydration)及抗原暴露 | BIONOVAS貨號LB0200-0200
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NOVA Histo (20X) 三合一脫石蠟、再水化(rehydration)及抗原暴露試劑 | 貨號LB0200-0200 (200ML)
一步脫石蠟、再水化(rehydration)及抗原暴露(antigen unmask)
免疫組織化學 (IHC) 染色之前，石蠟包埋組織切片(paraffin embedded tissue section)需完成進行脫石蠟(de-paraffin)、補液(rehydration)和抗原修復(antigen retrieval)等處理。傳統方法使用二甲苯( xylene)、100% 酒精(100% alcohol)和 retrieval buffer 來執行這些步驟。然而，這些化學品相對有毒，氣味不佳且價格昂貴。NOVA Histo是一種專業產品，可一步處理您的切片，從脫石蠟、再水化(rehydration)到抗原暴露(antigen unmask)。NOVA Histo不僅是替代二甲苯(xylene)的無毒產品，而且對環境友好，易於製備，易於使用處理。 NOVA Histo 可以完全節省您的設備、環境、健康、時間和金錢。
When performing a paraffin embedded tissue section, the de-paraffin, rehydration and antigen retrieval treatment should be done before performing immunohistochemistry (IHC) staining. Traditional method use xylene, 100% alcohol, and retrieval buffer to do these steps. However, these chemicals are relative toxic, smell not good and expensive. NOVA Histo is a specialized product IN ONE STEP to treat your section from de-paraffin, rehydration to antigen unmask. NOVA Histonot only a non-toxic product to replace xylene, it also environmental friendly, that can easily prepare and easy to disposal. NOVA Histo can totally save your equipment, environment, health, time, and money.
Protocol
A. For microwave method
1. Cut paraffin embedded section and expands it onto coated slide in hot water bath.
2. Dry the section on the slide drying hotplate at 45°C for to complete dried.
3. Dilute 20x NOVA Histo to 1x by distilled water.
4. Put the slide into microwave resistant plastic or glass staining slide rack.
5. Pour 1x NOVA Histo into staining slide rack. The used 1x NOVA Histo volume depend on the amount that can totally
immerse the slide.
6. Heat the rack in the microwave oven on medium power for 1.5-2 minutes or to boiling.
7. Stop heating; let the slide rack cooling until to the cloudy-like liquid disappear. (be careful to manipulate the hot rack)
8. Check the de-paraffin efficiency, if not satisfied, re-run the step 6-7 again.
9. Wash the slide by distill water or IHC wash buffer.
10. Continue the IHC staining step according to your protocol.
B. For autoclave equipment method
1. Cut paraffin embedded section and expands it onto coated slide in hot water bath.
2. Dry the section on the slide drying hotplate at 45°C for to complete dried.
3. Dilute 20x NOVA Histo to 1x by distilled water.
4. Put the slide into autoclave resistant plastic or glass staining slide rack.
5. Pour 1x NOVA Histo into staining slide rack. The used 1x NOVA Histo volume depend on the amount that can totally
immerse the slide.
6. Dewax the rack in the autoclave equipment at 121 °C for 30 minutes
7. Stop heating; let the slide rack cooling until to the cloudy-like liquid disappear. (be careful to manipulate the hot rack)
8. Check the de-paraffin efficiency, if not satisfied, re-run the step 6-7 again.
9. Wash the slide by distill water or IHC wash buffer.
10. Continue the IHC staining step according to your protocol.
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| 化合物 (Chemical) | AA0060-0500 | 醋酸溶液試劑級 (Acetic Acid Reagent Grade), 500ML | 500ML |
| 化合物 (Chemical) | AA0060-4000 | 醋酸溶液試劑級 (Acetic Acid Reagent Grade), 4L | 4L |
| 化合物 (Chemical) | AA0481-0500 | 低熔點瓊脂糖 (Agarose Low EEO Agarose) Low EEO, 500g | 500g |
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| 化合物 (Chemical) | AA1440-0050 | 抑肱酶 (Aprotinin) Molecular Biology 6000U/MG | 50mg |
| 化合物 (Chemical) | AA0530-0025 | 牛白蛋白 Albumin Bovine,Fraction V(BSA), 25g | 25g |
| 化合物 (Chemical) | AA0530-0100 | 牛白蛋白 Albumin Bovine,Fraction V(BSA), 100g | 100g |
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| 化合物 (Chemical) | AC0130-0500 | 氯化鈣 (Calcium Chloride Anhydrous) | 500g |
| 化合物 (Chemical) | AC0130-0500 | 氯化鈣(Calcium Chloride Anhydrous) | 500g |
| 化合物 (Chemical) | AC1270-0025 | 亮藍R-250 (Coomassie Blue R-250) | 25g |
| 化合物 (Chemical) | AC0130-0500 | CHAPS, Molecular Biology >99% 3-膽胺丙基二甲基胺-1-丙烷磺酸 | 5g |
| 化合物 (Chemical) | AD0470-0500 | 二甲亞碸 Dimethylsulfoxide (DMSO), Molecular Biology | 500ML |
| 化合物 (Chemical) | BM0171-0100 | DNase I 脫氧核醣核酸酶 | 100mg |
| 化合物 (Chemical) | BM0171-1000 | DNase I 脫氧核醣核酸酶 | 1g |
| 化合物 (Chemical) | BM0081-0250 | Dnase I (Rnase-free), 250U, 2u/ul | 125ul |
| 化合物 (Chemical) | AD0810-0005 | DTT二硫赤蘚醣醇 (DL-Dithiothreitol) 99.0% | 5g |
| 化合物 (Chemical) | AE0270-0010 | 溴化乙錠Ethidium bromide [EB] 10mg/ml Stock Solution | 10mL |
| 化合物 (Chemical) | AG0010-0100 | 抗生素G418 Disulfate Salt (>700ug/mg), Molecular Biology | 1g |
| 化合物 (Chemical) | AG0010-0500 | 抗生素G418 Disulfate Salt (>700ug/mg), Molecular Biology | 5g |
| 化合物 (Chemical) | AD0310-0500 | 葡萄糖Glucose Anhydrous | 500g |
| 化合物 (Chemical) | AG0260-1000 | 甘油Glycerol, Molecular Biology | 1L |
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| 化合物 (Chemical) | AH0043-0250 | HEPES Free Acid, 250g N-2-羥乙基哌嗪-N’-2-乙磺酸 | 250g |
| 化合物 (Chemical) | AH0150-0500 | 鹽酸 (HCL) Hydrochloride acid (HCL) | 500mL |
| 化合物 (Chemical) | CH0360108 | 過氧化氫 (Hydrogen Peroxide 35%), 500ml | 500ml |
| 化合物 (Chemical) | AH0311-1000 | Hygromycin B 希高黴素 | 1g |
| 化合物 (Chemical) | AI0160-0010 | IPTG , Molecular Biology 99.0% 異丙基硫代半乳糖吡喃糖苷 | 10g |
| 化合物 (Chemical) | AI0160-0100 | IPTG , Molecular Biology 99.0% 異丙基硫代半乳糖吡喃糖苷 | 100g |
| 化合物 (Chemical) | AI0335-0500 | IsoPropanol (IPA) 異丙醇 | 500mL |
| 化合物 (Chemical) | AI0335-4000 | IsoPropanol (IPA) 異丙醇 | 4L |
| 化合物 (Chemical) | AK0010-0010 | Kanamycin Sulfate (>750ug/mg), Molecular Biology 康黴素硫酸鹽 | 10g |
| 化合物 (Chemical) | AK0010-0025 | Kanamycin Sulfate (>750ug/mg), Molecular Biology 康黴素硫酸鹽 | 25g |
| 化合物 (Chemical) | AL0680-0010 | Lysozyme (>50,000U/mg) 溶菌酶 | 10g |
| 化合物 (Chemical) | AM0030-0500 | Magnesium Chlorice hexahydrate | 500g |
| 化合物 (Chemical) | AB0590-0025 | 2-巰基乙醇 (2-Mercaptoethanol) | 25mL |
| 化合物 (Chemical) | AM0815-0001 | MTT (Thiazoyl Blue Tetrazolium Bromide) 甲基噻唑二苯基四唑溴 | 1g |
| 化合物 (Chemical) | AM0815-0005 | MTT (Thiazoyl Blue Tetrazolium Bromide) 甲基噻唑二苯基四唑溴 | 5g |
| 化合物 (Chemical) | AM0780-0250 | MOPS Purity>99.5 | 250g |
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| 化合物 (Chemical) | AN0220-0025 | 硫酸新黴素 (Neomycin Sulfate), 25g | 25g |
| 化合物 (Chemical) | AN0490-0500 | 100% NP-40 (Nonidet P-40) 非變性去污劑 | 500mL |
| 化合物 (Chemical) | AP0930-0010 | PMSF 苯甲基磺醯氟 | 10g |
| 化合物 (Chemical) | BS100-014 | Potassium Carbonate 碳酸鉀 (K2CO) | 500g |
| 化合物 (Chemical) | AO1480-0500 | 氯化鉀 (Potassium Chloride) | 500g |
| 化合物 (Chemical) | AP1280-0500 | 氫氧化鉀 (Potassium Hydroxide) | 500g |
| 化合物 (Chemical) | AP1410-0500 | Potassium Phosphate Monobasic Anhydrous （KH2PO4） | 500g |
| 化合物 (Chemical) | AP1510-0500 | 硫酸鉀 (Potassium Sulfate) | 500g |
| 化合物 (Chemical)) | AP1070-0800 | 醋酸鉀 (Potassium Acetate) | 500g |
| 化合物 (Chemical) | AP1810-0100 | 蛋白酶K (Proteinase K) (>40U/mg) | 100mg |
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| 化合物 (Chemical) | RNase A (>67.6U/mg), Molecular Biology 核酸酶A | 100mg | |
| 化合物 (Chemical) | AR0170-0001 | RNase A Solution (20mg/ml), 1ml 核酸酶A | 1ml |
| 化合物 (Chemical) | AS0370-0500 | Sodium Bicarbonate Reagent Grade 碳酸氫鈉 | 500g |
| 化合物 (Chemical) | AS0450-0500 | Sodium Carbonate, 500g (Na2CO3) 碳酸鈉 | 500g |
| 化合物 (Chemical) | AS0480-1000 | 氯化鈉 (Sodium Chloride )Molecular Biology | 1Kg |
| 化合物 (Chemical) | AS0530-0500 | 檸檬酸鈉 (Sodium Citrate Trisodium Salt Dihydrate), 500g | 500g |
| 化合物 (Chemical) | AS0070-0250 | Sodium Dodecyl Sulfate (SDS), Molecular Biology 99% 十二烷基硫酸鈉 | 250g |
| 化合物 (Chemical) | AS0070-0500 | Sodium Dodecyl Sulfate (SDS), Molecular Biology 99% 十二烷基硫酸鈉 | 500g |
| 化合物 (Chemical) | AS0710-0500 | 氫氧化鈉 (Sodium Hydroxide) Reagent Grade, 500g | 500g |
| 化合物 (Chemical) | AS0930-0500 | Sodium Phosphate Dibasic Anhydrous 磷酸氫二鈉 Na2HPO4 | 500g |
| 化合物 (Chemical) | AS0940-0500 | Sodium Phosphate Monobasic Anhydrous 磷酸二氫鈉 NaH2PO4 | 500g |
| 化合物 (Chemical)) | AS1560-0500 | Sucrose Ultra Pure Grade 99% 蔗糖 | 500g |
| 化合物 (Chemical) | AS1560-1000 | Sucrose Ultra Pure Grade 99% 蔗糖 | 1Kg |
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| 化合物 (Chemical) | AT0890-1000 | Tris, Molecular Biology 三羥甲基氨基甲烷【77-86-1】 | 1Kg |
| 化合物 (Chemical) | AT1260-0500 | Tween 20 聚氧乙烯山梨醇酐月桂酸酯 | 500ML |
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| 化合物 (Chemical) | AX0020-1000 | X-Gal, Molecular Biology 99.0% 5溴4氯3吲哚吡喃半乳糖 | 1g |
| 微生物培養基 | EA0045-0500 | AGAR A 瓊脂 | 500G |
| 微生物培養基 | EA2530-0500 | TRYPTONE 蛋白腖培養基 | 500G |
| 微生物培養基 | EA1190-0500 | LB BROTH, MILLER 培養基 | 500G |
| 微生物培養基 | EA1160-0500 | LB AGAR BROTH 培養基 | 500G |
| 微生物培養基 | EA1980-0500 | PEPTONE | 500G |
| 微生物培養基 | EA2880-0500 | Yeast Extract 酵母培養基 | 500g |
| 分生試劑 | SDL-100R | Star-100bp DNA Ladder(Ready to use)‚72 ug 核酸標記 | 800ul |
| 分生試劑 | SDL-1000R | Star-1KB DNA Ladder(Ready to use)‚ 1360 ng/5μl 核酸標記 | 500ul |
| 分生試劑 | AM0670-1000 | Hiscript I (MMLV) RT 反轉錄酵素 | 10000U |
| 分生試劑 | AM0675-0050 | HiScript ITM First Strand cDNA Synthesis Kit (V3) 反轉錄套組 | 100rxns |
| 分生試劑 | BM0010-0001 | RNA-Defense™ Reagent(保存液) | 1ml |
| 分生試劑 | BM0020-0500 | RNApreserve™ Sample Protecting Reagent(保存液) | 500ml |
| 分生試劑 | BM0030-2000 | RNase Inhibitor 2000U RNA抑制劑 | 2000U |
| 分生試劑 | BM0040-0500 | D/RNAse-Eraser™ (去除液) | 500ML |
| 分生試劑 | CA0430-0001 | Oligo dT(18) primer 寡核甘酸引子 | 25ug |
| 分生試劑 | CA0360-0001 | Random Primers 引子 | 25ug |
| 分生試劑 | BM0100-0001 | 2.5mM dNTP Mix Solution 核甘酸 | 1ml |
| 分生試劑 | BM0110-0001 | 10mM dNTP Mix Solution 核甘酸 | 1ml |
| 分生試劑 | BM0130-0400 | 100mM dATP Solution 核甘酸 | 400ul |
| 分生試劑 | BM0140-0400 | 100mM dCTP Solution | 400ul |
| 分生試劑 | BM0150-0400 | 100mM dGTP Solution | 400ul |
| 分生試劑 | BM0160-0400 | 100mM dTTP Solution | 400ul |
| 分生試劑 | VA-102-T | DirectPCR Lysis Reagent 100ml(Tail) 試劑 | 100ML |
| 分生試劑 | BL0020-0001 | RNA 6X Loading Dye, 1ml 染劑 | 1ml |
| 分生試劑 | 6XDYE | DNA 6X Loading Dye, 1ml 染劑 | 1ml |
| 分生試劑 | AMP-A324402-1 | RealQ Plus 2x Master Mix Green Low ROX | 1.25ml |
| 分生試劑 | AMP-A325402-1 | RealQ Plus 2x Master Mix Green High ROX 螢光定量試劑 | 1.25ml |
| 分生試劑 | BN0150-0250 | VAS Taq, 5u/ul 核酸酵素 | 250u |
| 分生試劑 | AMP-180301-1 | 2.0x Taq Master (RED) 含dye 核酸反應混合液 | 1.25ml |
| 分生試劑 | 180701 | 2X FastTaq master mix w/dye Purple, 1ml | 1ml |
| 分生試劑 | AMP-A370501 | Taq 2x OptiMix CLEAR 1.5 mM | 1.25ml |
| 分生試劑 | AMP-A767501 | AQ97 High Fidelity DNA Polymerase With 5x AQ97 Buffer (7.5 mM MgCl2) | 1.25ml |
| 分生試劑 | A620601 | PureIT ExoZAP PCR CleanUp | 0.2ml |
| 分生試劑 | 5200500-1250 | TEMPase Hot Start 2x Master Mix C , 1.5 mM MgCl2 final concentration | 1.25ml |
| 分生試劑 | 5200600-1250 | TEMPase HS 2x Master Mix A BLUE | 1.25ml |
| 分生試劑 | BN0100-0001 | Hotstart Taq Premix (w/o dye), 1ml | 1ml |
| 分生試劑 | BM0300-G500 | Intercalating Dyes Destroyer, 500ml ETBR 去污噴劑 | 500ML |
| 分生試劑 | AS2100-1000 | SafeSense Nucleic Acid Staining Solution (10,000x) 核酸安全染劑 | 1ML |
| 分生試劑 | AS2110-1000 | Safe-Stain DNA Loading Buffer,1ML | 1ML |
| BUFFER | BL0240-0500 | 20% (w/v) Sodium Dodecyl Sulfate (SDS) Solution 十二烷基硫酸鈉溶液 | 500ml |
| BUFFER | BL0250-4000 | 20X SSC Buffer | 4L |
| BUFFER | BL0280-1000 | 50X TAE Buffer | 1L |
| BUFFER | BL0300-1000 | 5X TBE Buffer | 1L |
| BUFFER | BL0300-4000 | 5X TBE Buffer | 4L |
| BUFFER | BL0310-1000 | 10X TBS Buffer | 1L |
| BUFFER | BL0310-4000 | 10X TBS Buffer | 4L |
| BUFFER | BL0320-1000 | 10X TBST Buffer | 1L |
| BUFFER | BL0340-0500 | 1X TE Buffer | 500ML |
| BUFFER | BL0370-1000 | 10X TG Buffer | 1L |
| BUFFER | BL0370-4000 | 10X TG Buffer | 4L |
| BUFFER | BL0380-1000 | Tris-Glycine SDS Buffer (TGS) (10X) 緩衝液 | 1L |
| BUFFER | BL0380-4000 | Tris-Glycine SDS Buffer (TGS) (10X) | 4L |
| BUFFER | BL0180-1000 | 10X PBS Buffer, 1L 緩衝液 (sterile) | 1L |
| BUFFER | BL0170-4000 | 10X PBS Buffer, 4L 緩衝液 未滅菌 | 4L |
| BUFFER | BL0171-1000 | 1X PBS Buffer, 1L 緩衝液 (sterile) | 1L |
| BUFFER | BL0190-1000 | 10X PBST Buffer, 1L 緩衝液 | 1L |
| BUFFER | BL0415-1000 | 4% Paraformaldehyde Solution, | 1L |
| BUFFER | BL0460-1000 | 1.5M Tris-HCl Solution, pH6.8 | 1L |
| BUFFER | BL0350-1000 | 1.5M Tris-HCl Solution, pH8.8 | 1L |
| BUFFER | AT0950-1000 | 1M Tris-HCl Solution pH6.8, 1L | 1L |
| BUFFER | AT0990-1000 | 1M Tris-HCl Solution pH8.8, 1L | 1L |
| BUFFER | BL0340-1000 | 0.5M Tris-HCl Solution, pH6.8 | 1L |
| BUFFER | BL0470-1000 | 0.5M Tris-HCl Solution, pH8.8 | 1L |
| BUFFER | BL0100-1000 | Water, DEPC Treated | 1L |
| BUFFER | BL0110-0500 | Water, DNase/RNase Free | 500ml |
| BUFFER | BL0210-1000 | RedBloodCellLysisSolution(1X), 1L 紅血球分解溶液 | 1L |
| BUFFER | AM0440-4000 | Methanol 甲醇 | 4L |
| BUFFER | RA0300-1000 | 10X Go-Fast DNA Running Buffer, 1L | 1L |
| BUFFER | RA0370-1000 | 10X Go-Fast WB Transfer Buffer, 1L | 1L |
| 蛋白質表現 | FC0070-0001 | Protease Inhibitors Cocktail VII, Full Range (100X) 蛋白酶抑制劑 | 1mL |
| 蛋白質表現 | FC0050-0001 | Phosphatase Inhibitor Cocktail, Full Range (100X) 磷酸酶抑制劑 | 1ml |
| 蛋白質表現 | BL0220-0100 | RIPA Buffer (w/o Protease Inhibitor) 100ml 裂解液 | 100ML |
| 蛋白質表現 | FL0010-0170 | SuperLight Chemiluminescent HRP Kit (ECL) 冷光試劑 | 250ml*2 |
| 蛋白質表現 | AA0220-0500 | Acrylamide/Bis-Acrylamide 30% Solution (29:1), 500ml 電泳試劑 | 500ML |
| 蛋白質表現 | AA0230-0500 | Acrylamide/Bis-Acrylamide 30% Solution (37.5:1), 500ml 電泳試劑 | 500ML |
| 蛋白質表現 | IRIS11-250 | HLW Prestained Protein Ladder, | 250ul |
| 蛋白質表現 | BL0430-0500 | Stripping Buffer 免疫球蛋白剝離緩衝液 | 500ml |
| 蛋白質表現 | BL0420-0500 | SuperBlock Blocking Buffer, 500ml 封閉液 | 500ml |
| 蛋白質表現 | GS0010-0250 | Protein marker 10-180 kda 蛋白質標記 | 250 ul |
| 蛋白質表現 | FA0020-0025 | 5X Protein Sample Buffer 染劑 | 25ml |
| 細胞培養 | BP231-100 | THERMO Dimethyl sulfoxide (DMSO), 100ml (保存細胞用) | 100ML |
| 細胞培養 | ECM009DCLB | Amphotericin B | 100ML |
| 細胞培養 | FI0050-0250 | Nova Clean 水盤/水浴槽抗菌劑, 250ml | 250ML |
| 細胞培養 | FI0060-0250 | Nova Clean 水盤/水浴槽抗菌劑 無色, 250ml | 250ML |
| 細胞培養 | FI0060-0125 | Nova Clean 水盤/水浴槽抗菌劑 無色, 125ml | 125ML |
| 細胞培養 | AT1070-0010 | Trypan Blue Solution, 100ml | 100ml |