T4 DNA連接酶 | DNA Ligase貨號G467 1 U/μl 200 U (200 μl)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated interdiate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.

T4 DNA 連接酶(T4 DNA Ligase)催化在 dsDNA、dsRNA 或 DNA-RNA 雜交體的相鄰切口核苷酸的近端 3′- 羥基和 5′- 磷酸末端之間形成磷酸二酯鍵( phosphodiester bond )。已知通過腺苷酸化中間體(adenylated intermediate)進行，這種endergonic reaction可用於連接內聚末端( cohesive-ended )和平末端( blunt-ended termini)。

Description	T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
SKU	G467
Unit quantity	200 U (200 μl)
Applications	• Cloning of restriction fragments • Attaching double-stranded oligonucleotide linkers or adaptors to DNA • Site-directed mutagenesis • Nick repair of duplex DNA, RNA or DNA-RNA hybrids • Self-circularization of linear DNA
Concentration	1 U/μl
Format	Enzyme supplied with 5X Reaction Buffer
Storage Condition	Store all components at -20°C.
Note	This product is distributed for laboratory research only. Caution: Not for diagnostic use.