Fibronectin (FN)活性重組蛋白 |Active Fibronectin (FN)貨號APA037Hu01
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Fibronectin (FN)活性重組蛋白 | Active Fibronectin (FN)貨號APA037Hu01
FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein
產品介紹: Cloud clone提供原核及真核生物表現之重組蛋白系列商品；其中Active Fibronectin (FN)活性重組蛋白，為活性蛋白，Organism Species Homo sapiens (Human) 。純度> 97%，提供凍乾型式包裝(Freeze-dried powder)。可應用於Cell culture; Activity Assays等不同研究。
蛋白質保存型式
蛋白質溶於20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.為冷凍乾燥包裝。使用前Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.避免vortex. 短期保存Store at 2-8°C for one month.分裝-80°C長期12 個月。請避免反覆冷凍解凍。
纖連蛋白 (Fibronectin , FN) 是細胞外基質(extracellular matrix)的一種高分子量 (~440kDa) 糖蛋白(glycoprotein)，可與稱為整合素(integrins)的跨膜受體蛋白(membrane-spanning receptor proteins)結合。纖連蛋白(Fibronectins, FN)結合細胞表面(cell surfaces)和各種化合物(various compounds)，包括膠原蛋白(collagen)、纖維蛋白(fibrin)、肝素(heparin)、DNA 和肌動蛋白。纖連蛋白(Fibronectins, FN)具有多種功能。例如，它參與細胞粘附( cell adhesion)、細胞運動(cell motility)、調理作用(opsonization)、傷口癒合(wound healing)、細胞形態維持(maintenance of cell shape)等。此外，核心蛋白聚醣 (DCN) 已被確定為 纖連蛋白(Fibronectins, FN)的相互作用因子，因此進行結合 ELISA 測定以檢測重組人 纖連蛋白(Fibronectins, FN) 和重組人 DCN 的相互作用。簡而言之，將 FN 在含有 0.01% BSA (pH 7.4) 的 PBS 中連續稀釋。然後將 100uL 的重複樣品轉移到 DCN 包被的微量滴定孔中，並在 37℃ 下孵育 2 小時。用PBST洗滌孔並用抗-纖連蛋白(Fibronectins, FN) pAb孵育1小時，然後吸出並洗滌3次。用HRP標記的二抗孵育後，將孔吸出並洗滌3次。加入底物溶液，37℃孵育15-25分鐘。最後，向孔中加入 50µL 終止液，立即在 450nm 處讀數。纖連蛋白(Fibronectins, FN)和DCN的結合活性如圖1所示，這種作用呈劑量依賴性。
Fibronectin (FN)活性重組蛋白相關之抗體 | 蛋白質 產品
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APA037Hu02 | Active Fibronectin (FN) | Cell culture; Activity Assays. |
| RPA037Hu03 | Recombinant Fibronectin (FN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA037Hu61 | Active Fibronectin (FN) | Cell culture; Activity Assays. |
| EPA037Hu61 | Eukaryotic Fibronectin (FN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA037Hu01 | Active Fibronectin (FN) | Cell culture; Activity Assays. |
| RPA037Hu01 | Recombinant Fibronectin (FN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA037Hu02 | Recombinant Fibronectin (FN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA037Hu01 | Polyclonal Antibody to Fibronectin (FN) | WB; IHC; ICC; IP. |
| PAA037Hu04 | Polyclonal Antibody to Fibronectin (FN) | WB; IHC; ICC; IP. |
| PAA037Hu02 | Polyclonal Antibody to Fibronectin (FN) | WB; IHC; ICC; IP. |
| PAA037Hu03 | Polyclonal Antibody to Fibronectin (FN) | WB; IHC; ICC; IP. |
| LAA037Hu71 | Biotin-Linked Polyclonal Antibody to Fibronectin (FN) | WB; IHC; ICC. |
| MAA037Hu22 | Monoclonal Antibody to Fibronectin (FN) | WB; IHC; ICC; IP. |
| SEA037Hu | ELISA Kit for Fibronectin (FN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA037Hu | High Sensitive ELISA Kit for Fibronectin (FN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA037Hu | CLIA Kit for Fibronectin (FN) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA037Hu | Magnetic Luminex Assay Kit for Fibronectin (FN) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSA037Hu01 | ELISA Kit DIY Materials for Fibronectin (FN) | Main materials for “Do It (ELISA Kit) Yourself”. |