蛋白酶 K (Proteinase K) | CAS#39450-01-6 Abbexa  貨號 abx082041

分子生物應用建議量: 50-100 µg/ml

蛋白酶 K (Proteinase K)是一種廣譜(broad spectrum) 絲氨酸蛋白酶(serine protease)，在 DNA 提取方案中用於消化污染蛋白質(contaminating proteins)，尤其是 DNA 酶(DNases)。該酶裂解蛋白質中與脂肪族(aliphatic)和芳香族氨基酸殘基(aromatic amino acid residues)的羧基相鄰的肽鍵(peptide bonds)。蛋白酶 K(Proteinase K) 的主要應用是在 DNA 和 RNA 提取(RNA extraction)中的應用。在大多數核酸提取 (nucleic acid extraction)方案中，蛋白酶 K (Proteinase K)被添加到組織(tissue)或裂解物(the lysate)中，以確保在核酸提取過程中完全消化蛋白質，尤其是 DNase 和 RNase。消化蛋白質(digest proteins)的能力也使蛋白酶 K 可用於製備用於脈衝場凝膠電泳 (pulsed-field gel electrophoresis)、蛋白質指紋(protein fingerprinting)、蛋白酶足印(protease foot printing)、線粒體分離(mitochondrial isolation)、基因組 DNA (genomic DNA isolation)分離等的染色體 DNA(chromosomal DNA)。

Target	Proteinase K
Expression	Recombinant
Host	Yeast
Recommended dilutions	Molecular biology applications: 50-100 µg/ml. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Form	Lyophilized
Purity	≥ 95% (SDS-PAGE) Free from DNase and RNase.
Reconstitution	Reconstitute in a 50% glycerin solution. Ensure that the powder has fully dissolved before use. Use immediately after preparation, or store at 2-8 °C. For long-term storage, reconstitute to a concentration of 10-20 mg/ml and store at -20 °C.
Storage	Store between 2-8 °C for up to 2 years.
Enzyme Commission Number	3.4.21.64
Molecular Weight	29 kDa
Buffer	Prior to lyophilization: 10 mM Tris-HCl, pH 7.5, containing 5 mM CaCl2.
CAS Number	39450-01-6
Biological Activity	≥ 30 U/mg 1 Unit (U) is defined as the amount of Proteinase K required to produce 1 µmol of Folin-positive amino acids using hemoglobin as the substrate, in 1 min at 37 °C.
Directions for use	Effective pH range: 4-12.5 Optimal pH range: 7.5-8.0 Optimal reaction temperature: 50-55 °C
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.Not for human consumption, cosmetic, therapeutic or diagnostic use.