PC3 細胞外泌體(exosome)標準液| AMSBIO貨號M1060-100-2
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【外泌體(Exosomes)標準液】Lyophilized Exosome Standards- Amsbio
PC3 細胞外泌體(exosome)標準液| AMSBIO貨號M1060-100-2
Lyophilized exosomes from PC3 cell line
外泌體(exosome)標準液
凍乾的外泌體(Lyophilized Exosomes) 純化通過切向流過濾 (tangential flow filtration ,TFF)、結合體積排阻層析 (size exclusion chromatography ,SEC)。分離的囊泡(Isolated vesicles)進行總蛋白質含量量化和，常見標記表達（CD9、CD81、CD63）驗證(validated)。使用 Zetaview 通過 NTA納米粒子跟踪分析（Nanoparticles Tracking Analysis）Particle Metrix（粒子矩陣）粒徑分佈、濃度。凍乾方式不會改變外泌體(exosome) 蛋白質和核酸穩定性。與其他儲存相比，包括在 -20°C 下儲存新鮮外泌體(exosome)。凍乾的外泌體(exosome)易於運輸且穩定，可長期儲存（最多 36月）。
外泌體(Exosomes)是小的核內體衍生的脂質納米顆粒(small endosome derived lipid nanoparticles)，在大多數活細胞中通過胞吐作用(exocytosis)積極分泌。它們通過裝載 RNA(cargo of RNA)、蛋白質(proteins)、脂質(lipids)和 DNA 發揮信號載體(signal carriers)的作用。外泌體參與廣泛的正常細胞和致病過程，包括癌症(cancer)、炎症(inflammation)、免疫(immunity)、中樞神經系統功能(CNS function)和心肌細胞功能(cardiac cell function)，是組織工程(tissue engineering)和再生醫學(regenerative medicine)應用的有吸引力的起始材料。
產品介紹:
外泌體研究(exosome research)提供多種即用型試劑盒(ready-to-use kits)，包括定量試劑盒(quantification kits)、分離試劑盒(isolation kits)和 DNA/RNA 提取試劑盒(DNA/RNA extraction kits)，其中包括用於腫瘤源性免疫捕獲(tumor-derived immunocapture)和 RNA 提取的試劑盒(RNA extraction)、外泌體(exosome)標準液。
外泌體(exosome)特性：
外泌體是大多數活細胞通過胞吐作用(exocytosis)主動分泌的 EV，通常報告的直徑在 40 到 120 nm之間。外泌體(Exosome)釋放發生在組成型(constitutively)或誘導(induction)時，在兩種情況下正常和病理狀況。數量(quantity)和分子(molecular)釋放的外泌體(released exosomes)的組成取決於生理狀態(physiological state)的親代細胞(parental cells)。
外泌體(exosome)標準液 Our ExoStd Process：
Lyophilized Exosome Standards from human Biofluids (plasma, serum, urine, saliva) of healthy donors. • Lyophilized Exosomes from cell culture media (COLO1, MM1, BLCL21, HCT116, SK-N-SH, U87, PC3, BPH-1, DAUDI, A549, K562, mouse cell B16F10). • Lyophilized Exosomes from Human Mesenchymal Stem Cells (MSC) from adipose tissue. Pool of 10 different donors. • Lyophilized Exosome Standards size avalable: 100 μg and 30 μg, sold in packages of 2, 5 vials.
Applications
- Positive control for marker assessment
- Control (spike-in) for EV quantification
- OMICS analysis
- Standardized positive controls for immunocapture performance evaluation
- Flow cytometry
- Electron microscopy
Characteristics
- High purity
- Size distribution: 50 – 120 nm
- Long term stability at 4-8° C
- Purified from biofluids collected from certified donor pools
- Purified using combination of TFF and SEC
Advantages
- Easy to reconstitute
- Easy to ship and store (+4°C)
- Long term storage stability (36 months)
- Marker characterized
- Available from a large cell line bank
三種類型- 外泌體標準品：
- 來自人類生物體液(human Biofluids)（血漿、健康捐贈者的血清、尿液、唾液）。
- 來自細胞培養基的凍乾外泌體（COLO1、MM1、BLCL21、HCT116、SK-N-SH、U87、PC3、BPH-1、DAUDI、A549、K562，小鼠細胞 B16F10）。
- 來自脂肪組織之間質幹細胞（Mesenchymal stem cell，MSC)之凍乾外泌體。
Our ExoStd Products (Fluorescent labelling available for all standards)
7a- Marker detection by Western Blotting.
Reconstituted Exosomes can be directly lysed in Laemmli buffer, then loaded on the Electrophoresis gel. Recommended quantity: 10-20 μg per line.
7b- Phenotyping by ELISA assay.
Reconstituted Exosomes can be loaded directly onto ELISA plate wells. Recommended quantity: 10 – 20 μg per well.
7c- NTA in fluorescence and scattered mode.
Reconstituted Exosomes can be used for phenotyping assays by fluorescence NTA. Recommended quantity: 10 μg of Exosomes incubated with 1 μg* of antibody fluorophore conjugated or 2 μl* of membrane dye CMDR.
7e- Profiling of Exosome associated nucleic acids.
Reconstituded Exosomes can be used for profiling nucleic acids (RNAs and DNA) biomarkers. The lysis buffer can be added directly to the Exosome preparation. Recommended quantity: 20-50 μg per reaction.
7d- Phenotyping assays by FACS
Reconstituted Exosomes can be used for profiling biomarkers by FACS analysis. Recommended quantity: 5 μg of reconstituted Exosomes Standards for each test.
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| Lyophilized exosomes from A549 cell line, M1066-100-2 |  |
2×100µg vial | View |
| Lyophilized exosomes from A549 cell line, M1066-2 | |
2×30µg vial | View |
| Lyophilized exosomes from A549 cell line, M1066-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from A549 cell line, M1066-5 | |
5×30µg vial | View |
| Lyophilized exosomes from B16F10 cell line, M1070-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from B16F10 cell line, M1070-2 | |
2×30µg vial | View |
| Lyophilized exosomes from B16F10 cell line, M1070-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from B16F10 cell line, M1070-5 | |
5×30µg vial | View |
| Lyophilized exosomes from COLO1 cell line, M1048-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from COLO1 cell line, M1048-2 | |
2×30µg vial | View |
| Lyophilized exosomes from COLO1 cell line, M1048-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from COLO1 cell line, M1048-5 | |
5×30µg vial | View |
| Lyophilized exosomes from HCT116 cell line, M1058-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from HCT116 cell line, M1058-2 | |
2×30µg vial | View |
| Lyophilized exosomes from HCT116 cell line, M1058-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from HCT116 cell line, M1058-5 | |
5×30µg vial | View |
| Lyophilized exosomes from HEK293 cell line, M1047-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from HEK293 cell line, M1047-2 | |
2×30µg vial | View |
| Lyophilized exosomes from HEK293 cell line, M1047-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from HEK293 cell line, M1047-5 | |
5×30µg vial | View |
| Lyophilized exosomes from HT29 cell line, M1049-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from HT29 cell line, M1049-2 | |
2×30µg vial | View |
| Lyophilized exosomes from HT29 cell line, M1049-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from HT29 cell line, M1049-5 | |
5×30µg vial | View |
| Lyophilized exosomes from Human Serum of Healthy donors, M1049-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from Human Serum of Healthy donors, M1049-2 | |
2×30µg vial | View |
| Lyophilized exosomes from Human Serum of Healthy donors, M1049-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from Human Serum of Healthy donors, M1049-5 | |
5×30µg vial | View |
| Lyophilized exosomes from K562 GM cell line, M1068-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from K562 GM cell line, M1068-2 | |
2×30µg vial | View |
| Lyophilized exosomes from K562 GM cell line, M1068-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from K562 GM cell line, M1068-5 | |
5×30µg vial | View |
| Lyophilized exosomes from LnCAP cell line, M1061-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from LnCAP cell line, M1061-2 | |
2×30µg vial | View |
| Lyophilized exosomes from LnCAP cell line, M1061-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from LnCAP cell line, M1061-5 | |
5×30µg vial | View |
| Lyophilized exosomes from MSC from human adipose tissue, M1041-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from MSC from human adipose tissue, M1041-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from NCI-H1975 cell line, M1062-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from NCI-H1975 cell line, M1062-2 | |
2×30µg vial | View |
| Lyophilized exosomes from NCI-H1975 cell line, M1062-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from NCI-H1975 cell line, M1062-5 | |
5×30µg vial | View |
| Lyophilized exosomes from PC3 cell line, M1060-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from PC3 cell line, M1060-2 | |
2×30µg vial | View |
| Lyophilized exosomes from PC3 cell line, M1060-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from PC3 cell line, M1060-5 | |
5×30µg vial | View |
| Lyophilized exosomes from Plasma of Healthy donors, M1040-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from Plasma of Healthy donors, M1040-2 | |
2×30µg vial | View |
| Lyophilized exosomes from Plasma of Healthy donors, M1040-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from Plasma of Healthy donors, M1040-5 | |
5×30µg vial | View |
| Lyophilized exosomes from SK-N-SH cell line, M1056-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from SK-N-SH cell line, M1056-2 | |
2×30µg vial | View |
| Lyophilized exosomes from SK-N-SH cell line, M1056-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from SK-N-SH cell line, M1056-5 | |
5×30µg vial | View |
| Lyophilized exosomes from U87 GM cell line, M1054-100-2 | |
2×100µg vial | View |
| Lyophilized exosomes from U87 GM cell line, M1054-2 | |
2×30µg vial | View |
| Lyophilized exosomes from U87 GM cell line, M1054-100-5 | |
5×100µg vial | View |
| Lyophilized exosomes from U87 GM cell line, M1054-5 | |
5×30µg vial | View |
| Lyophilized exosomes from Urine of Healthy donors, M1045-2 | |
2×100µg vial | View |
| Lyophilized exosomes from Urine of Healthy donors, M1044-2 | |
2×30µg vial | View |
| Lyophilized exosomes from Urine of Healthy donors, M1045-5 | |
5×100µg vial | View |
| Lyophilized exosomes from Urine of Healthy donors, M1044-5 | |
5×30µg vial | View |
| Lyophilized microvesicles from B16F10 mouse cell line, M3010 | |
50µg vial | View |
| Lyophilized microvesicles from COLO1 cell line | |
50µg vial | View |
| Lyophilized microvesicles from HCT116 cell line | |
50µg vial | View |
| Lyophilized microvesicles from HEK293 cell line | |
50µg vial | View |
| Lyophilized microvesicles from K562 cell line | |
50µg vial | View |
| Lyophilized microvesicles from LnCAP-1 cell line | |
50µg vial | View |
| Lyophilized microvesicles from NCI-H1975 cell line | |
50µg vial | View |
| Lyophilized microvesicles from PC3 cell line | |
50µg vial | View |
| Lyophilized microvesicles from SK-N-SH cell line | |
50µg vial | View |
| Lyophilized microvesicles from U87 GM cell line | |
50µg vial | View |
| Lyophilized microvesicles fromA549 cell line | |
50µg vial | View |
Amsbio台灣代理商-BRAND/AGENCY/代理品牌- Amsbio
AMSBIO於基因體學、蛋白質體學、細胞培養和幹細胞等領域均有涉略。 除了針對細胞增殖、貼附、轉移和侵入等研究細胞基礎功能之產品，另外還提供正常人及疾病患者的生物性樣本，例如乳癌、結腸癌等，以符合研究者的需求。產品線包含DNA/RNA/蛋白質/組織切片樣本、3D細胞培養、抗體及各式分析試劑盒等。