HCT116細胞外泌體(exosome)標準液| AMSBIO貨M1058-100-2
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【外泌體(Exosomes)標準液】Lyophilized Exosome Standards- Amsbio
HCT116細胞外泌體(exosome)標準液| AMSBIO貨M1058-100-2
Lyophilized exosomes from HCT116 cell line
外泌體(exosome)標準液
凍乾的外泌體(Lyophilized Exosomes) 純化通過切向流過濾 (tangential flow filtration ,TFF)、結合體積排阻層析 (size exclusion chromatography ,SEC)。分離的囊泡(Isolated vesicles)進行總蛋白質含量量化和，常見標記表達（CD9、CD81、CD63）驗證(validated)。使用 Zetaview 通過 NTA納米粒子跟踪分析（Nanoparticles Tracking Analysis）Particle Metrix（粒子矩陣）粒徑分佈、濃度。凍乾方式不會改變外泌體(exosome) 蛋白質和核酸穩定性。與其他儲存相比，包括在 -20°C 下儲存新鮮外泌體(exosome)。凍乾的外泌體(exosome)易於運輸且穩定，可長期儲存（最多 36月）。
外泌體(Exosomes)是小的核內體衍生的脂質納米顆粒(small endosome derived lipid nanoparticles)，在大多數活細胞中通過胞吐作用(exocytosis)積極分泌。它們通過裝載 RNA(cargo of RNA)、蛋白質(proteins)、脂質(lipids)和 DNA 發揮信號載體(signal carriers)的作用。外泌體參與廣泛的正常細胞和致病過程，包括癌症(cancer)、炎症(inflammation)、免疫(immunity)、中樞神經系統功能(CNS function)和心肌細胞功能(cardiac cell function)，是組織工程(tissue engineering)和再生醫學(regenerative medicine)應用的有吸引力的起始材料。
產品介紹:
外泌體研究(exosome research)提供多種即用型試劑盒(ready-to-use kits)，包括定量試劑盒(quantification kits)、分離試劑盒(isolation kits)和 DNA/RNA 提取試劑盒(DNA/RNA extraction kits)，其中包括用於腫瘤源性免疫捕獲(tumor-derived immunocapture)和 RNA 提取的試劑盒(RNA extraction)、外泌體(exosome)標準液。
外泌體(exosome)特性：
外泌體是大多數活細胞通過胞吐作用(exocytosis)主動分泌的 EV，通常報告的直徑在 40 到 120 nm之間。外泌體(Exosome)釋放發生在組成型(constitutively)或誘導(induction)時，在兩種情況下正常和病理狀況。數量(quantity)和分子(molecular)釋放的外泌體(released exosomes)的組成取決於生理狀態(physiological state)的親代細胞(parental cells)。
外泌體(exosome)標準液 Our ExoStd Process：
Lyophilized Exosome Standards from human Biofluids (plasma, serum, urine, saliva) of healthy donors. • Lyophilized Exosomes from cell culture media (COLO1, MM1, BLCL21, HCT116, SK-N-SH, U87, PC3, BPH-1, DAUDI, A549, K562, mouse cell B16F10). • Lyophilized Exosomes from Human Mesenchymal Stem Cells (MSC) from adipose tissue. Pool of 10 different donors. • Lyophilized Exosome Standards size avalable: 100 μg and 30 μg, sold in packages of 2, 5 vials.
Applications
- Positive control for marker assessment
- Control (spike-in) for EV quantification
- OMICS analysis
- Standardized positive controls for immunocapture performance evaluation
- Flow cytometry
- Electron microscopy
Characteristics
- High purity
- Size distribution: 50 – 120 nm
- Long term stability at 4-8° C
- Purified from biofluids collected from certified donor pools
- Purified using combination of TFF and SEC
Advantages
- Easy to reconstitute
- Easy to ship and store (+4°C)
- Long term storage stability (36 months)
- Marker characterized
- Available from a large cell line bank
三種類型- 外泌體標準品：
- 來自人類生物體液(human Biofluids)（血漿、健康捐贈者的血清、尿液、唾液）。
- 來自細胞培養基的凍乾外泌體（COLO1、MM1、BLCL21、HCT116、SK-N-SH、U87、PC3、BPH-1、DAUDI、A549、K562，小鼠細胞 B16F10）。
- 來自脂肪組織之間質幹細胞（Mesenchymal stem cell，MSC)之凍乾外泌體。
Our ExoStd Products (Fluorescent labelling available for all standards)
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| Lyophilized exosomes from A549 cell line |  |
2×100µg vial | View |
| Lyophilized exosomes from A549 cell line | |
2×30µg vial | View |
| Lyophilized exosomes from A549 cell line | |
5×100µg vial | View |
| Lyophilized exosomes from A549 cell line | |
5×30µg vial | View |
| Lyophilized exosomes from B16F10 cell line | |
2×100µg vial | View |
| Lyophilized exosomes from B16F10 cell line | |
2×30µg vial | View |
| Lyophilized exosomes from B16F10 cell line | |
5×100µg vial | View |
| Lyophilized exosomes from B16F10 cell line | |
5×30µg vial | View |
| Lyophilized exosomes from COLO1 cell line | |
2×100µg vial | View |
| Lyophilized exosomes from COLO1 cell line | |
2×30µg vial | View |
| Lyophilized exosomes from COLO1 cell line | |
5×100µg vial | View |
| Lyophilized exosomes from COLO1 cell line | |
5×30µg vial | View |
| Lyophilized exosomes from HCT116 cell line | |
2×100µg vial | View |
| Lyophilized exosomes from HCT116 cell line | |
2×30µg vial | View |
| Lyophilized exosomes from HCT116 cell line | |
5×100µg vial | View |
| Lyophilized exosomes from HCT116 cell line | |
5×30µg vial | View |
| Lyophilized exosomes from HEK293 cell line | |
2×100µg vial | View |
| Lyophilized exosomes from HEK293 cell line | |
2×30µg vial | View |
| Lyophilized exosomes from HEK293 cell line | |
5×100µg vial | View |
| Lyophilized exosomes from HEK293 cell line | |
5×30µg vial | View |
| Lyophilized exosomes from HT29 cell line | |
2×100µg vial | View |
| Lyophilized exosomes from HT29 cell line | |
2×30µg vial | View |
| Lyophilized exosomes from HT29 cell line | |
5×100µg vial | View |
| Lyophilized exosomes from HT29 cell line | |
5×30µg vial | View |
| Lyophilized exosomes from Human Serum of Healthy donors | |
2×100µg vial | View |
| Lyophilized exosomes from Human Serum of Healthy donors | |
2×30µg vial | View |
| Lyophilized exosomes from Human Serum of Healthy donors | |
5×100µg vial | View |
| Lyophilized exosomes from Human Serum of Healthy donors | |
5×30µg vial | View |
| Lyophilized exosomes from K562 GM cell line | |
2×100µg vial | View |
| Lyophilized exosomes from K562 GM cell line | |
2×30µg vial | View |
| Lyophilized exosomes from K562 GM cell line | |
5×100µg vial | View |
| Lyophilized exosomes from K562 GM cell line | |
5×30µg vial | View |
| Lyophilized exosomes from LnCAP cell line | |
2×100µg vial | View |
| Lyophilized exosomes from LnCAP cell line | |
2×30µg vial | View |
| Lyophilized exosomes from LnCAP cell line | |
5×100µg vial | View |
| Lyophilized exosomes from LnCAP cell line | |
5×30µg vial | View |
| Lyophilized exosomes from MSC from human adipose tissue | |
2×100µg vial | View |
| Lyophilized exosomes from MSC from human adipose tissue | |
5×100µg vial | View |
| Lyophilized exosomes from NCI-H1975 cell line | |
2×100µg vial | View |
| Lyophilized exosomes from NCI-H1975 cell line | |
2×30µg vial | View |
| Lyophilized exosomes from NCI-H1975 cell line | |
5×100µg vial | View |
| Lyophilized exosomes from NCI-H1975 cell line | |
5×30µg vial | View |
| Lyophilized exosomes from PC3 cell line | |
2×100µg vial | View |
| Lyophilized exosomes from PC3 cell line | |
2×30µg vial | View |
| Lyophilized exosomes from PC3 cell line | |
5×100µg vial | View |
| Lyophilized exosomes from PC3 cell line | |
5×30µg vial | View |
| Lyophilized exosomes from Plasma of Healthy donors | |
2×100µg vial | View |
| Lyophilized exosomes from Plasma of Healthy donors | |
2×30µg vial | View |
| Lyophilized exosomes from Plasma of Healthy donors | |
5×100µg vial | View |
| Lyophilized exosomes from Plasma of Healthy donors | |
5×30µg vial | View |
| Lyophilized exosomes from SK-N-SH cell line | |
2×100µg vial | View |
| Lyophilized exosomes from SK-N-SH cell line | |
2×30µg vial | View |
| Lyophilized exosomes from SK-N-SH cell line | |
5×100µg vial | View |
| Lyophilized exosomes from SK-N-SH cell line | |
5×30µg vial | View |
| Lyophilized exosomes from U87 GM cell line | |
2×100µg vial | View |
| Lyophilized exosomes from U87 GM cell line | |
2×30µg vial | View |
| Lyophilized exosomes from U87 GM cell line | |
5×100µg vial | View |
| Lyophilized exosomes from U87 GM cell line | |
5×30µg vial | View |
| Lyophilized exosomes from Urine of Healthy donors | |
2×100µg vial | View |
| Lyophilized exosomes from Urine of Healthy donors | |
2×30µg vial | View |
| Lyophilized exosomes from Urine of Healthy donors | |
5×100µg vial | View |
| Lyophilized exosomes from Urine of Healthy donors | |
5×30µg vial | View |
| Lyophilized microvesicles from B16F10 mouse cell line | |
50µg vial | View |
| Lyophilized microvesicles from COLO1 cell line | |
50µg vial | View |
| Lyophilized microvesicles from HCT116 cell line | |
50µg vial | View |
| Lyophilized microvesicles from HEK293 cell line | |
50µg vial | View |
| Lyophilized microvesicles from K562 cell line | |
50µg vial | View |
| Lyophilized microvesicles from LnCAP-1 cell line | |
50µg vial | View |
| Lyophilized microvesicles from NCI-H1975 cell line | |
50µg vial | View |
| Lyophilized microvesicles from PC3 cell line | |
50µg vial | View |
| Lyophilized microvesicles from SK-N-SH cell line | |
50µg vial | View |
| Lyophilized microvesicles from U87 GM cell line | |
50µg vial | View |
| Lyophilized microvesicles fromA549 cell line | |
50µg vial | View |
Amsbio台灣代理商-BRAND/AGENCY/代理品牌- Amsbio
AMSBIO於基因體學、蛋白質體學、細胞培養和幹細胞等領域均有涉略。 除了針對細胞增殖、貼附、轉移和侵入等研究細胞基礎功能之產品，另外還提供正常人及疾病患者的生物性樣本，例如乳癌、結腸癌等，以符合研究者的需求。產品線包含DNA/RNA/蛋白質/組織切片樣本、3D細胞培養、抗體及各式分析試劑盒等。