人透明質酸 (HA) ELISA 試劑盒 | 貨號AE40537HU
人透明質酸 (HA) ELISA 試劑盒 | 貨號AE40537HU
Human Hyaluronic acid (HA) ELISA Kit
該測定採用雙位點夾心(two-site sandwich ELISA) ELISA 來定量樣品中的 透明質酸(Hyaluronic acid , HA)。 透明質酸(Hyaluronic acid , HA)特異性抗體已預先包被在微孔板上。標準品和樣品被移液到孔中，任何存在的 透明質酸(Hyaluronic acid , HA)都被固定的抗體結合。在去除任何未結合的物質後，將一種生物素偶聯(biotin-conjugated antibody)的 HA 特異性抗體添加到孔中。洗滌後，將鏈黴親和素綴合的辣根過氧化物酶 (HRP) 添加到孔中。在洗滌以去除任何未結合的抗生物素蛋白酶試劑後，將底物溶液添加到孔中，顏色與初始步驟中結合的透明質酸(Hyaluronic acid , HA) 的量成比例。停止顯色並測量顏色的強度。該方法對人透明質酸(Hyaluronic acid , HA)的檢測具有高靈敏度和優異的特異性。未觀察到人 透明質酸(Hyaluronic acid , HA)和類似物之間的顯著交叉反應(cross-reactivity)或干擾(interference)。
| Species Reactivity | Human (Homo sapiens) |
| UniProt | |
| Abbreviation | HA |
| Alternative Names | N/A |
| Range | 1.56-100 ng/mL |
| Sensitivity | 0.78 ng/mL |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Sandwich |
| Analysis Method | Quantitive |
| Assay Duration | 1-4.5h |
| Sample Volume | 1-200 μL |
| Detection Wavelengt | 450 nm |
| Reagents | Quantity | Reagents | Quantity |
| Assay plate (96 Wells) | 1 | Instruction manual | 1 |
| Standard (lyophilized) | 2 | Sample Diluent | 1 x 20 mL |
| Biotin-Conjugate (concentrate 100 x) | 1 x 120 μL | Biotin-Conjugate Diluent | 1 x 20 mL |
| Streptavidin-HRP (concentrate 100 x) | 1 x 120 μL | Streptavidin-HRP Diluent | 1 x 20 mL |
| Wash Buffer (concentrate 25 x) | 1 x 20 mL | Substrate Solution | 1 x 12 mL |
| Stop Solution | 1 x 10 mL | Adhesive Films | 4 |
Matrices listed below were spiked with certain level of recombinant Human HA and the recovery rates were calculated by comparing the measured value to the expected amount of Human HA in samples.
| Sample Type | Number | Recovery range (%) | Average(%) |
| Serum | 10 | 90-101 | 96 |
| EDTA plasma | 10 | 89-97 | 93 |
| Heparin plasma | 10 | 91-99 | 95 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
| Sample Type | 1：2 | 1：4 | 1：8 | 1：16 |
| Serum | 78-89% | 81-99% | 92-103% | 95-105% |
| EDTA plasma | 91-101% | 90-98% | 93-101% | 91-98% |
| Heparin plasma | 92-103% | 93-102% | 92-99% | 91-101% |