ExKine™ 粒線體提取試劑盒（組織） | ExKine™ Mitochondrion Extraction Kit (Tissue)貨號KTP4004

粒線體(Mitochondria)是一種雙膜結合(double-membrane-bound)的細胞器(organelle)，存在於大多數真核細胞(eukaryotic cells)中。粒線體(mitochondria)的功能是提供細胞能量(cellular energy)。此外，粒線體(Mitochondria)還參與其他任務，例如信號傳導(signaling)、細胞分化(cellular differentiation)和細胞死亡(cell death)，以及維持對細胞週期(cell cycle)和細胞生長(cell growth)的控制。粒線體(Mitochondria)與多種人類疾病有關，包括粒線體疾病(mitochondrial disorders)、心臟功能障礙(cardiacdysfunction)、心力衰竭(heart failure)和自閉症(autism)。粒線體(Mitochondria)可能在這些細胞過程中發揮重要作用。

Abbkine ExKine™ 線粒體提取試劑盒（組織）使用差速離心法從軟組織和硬組織的動物組織中，快速粗略地分離出完整的粒線體(Mitochondria)。這種粗粒線體(Mitochondria)製備通常足以滿足大多數應用，例如研究粒線體(Mitochondria)呼吸、粒線體(Mitochondria)膜電位、細胞凋亡、mtDNA 和 mtRNA，以及粒線體(Mitochondria)蛋白質分析等。

預計樣品的蛋白質濃度應約為 10-25 mg/mL

 

Fig. Mouse liver tissues were extracted with ExKine™ Mitochondrion Extraction Kit (Tissue). Mitochondrial (M) and Cytosolic (C) fractions were analyzed by WB using cytochrome c antibody (cat#ABM40191) and VDAC1 antibody (cat#ABP53121).

Product name	ExKine™ Mitochondrion Extraction Kit (Tissue)
Applications notes	Abbkine ExKine™ Mitochondrion Extraction Kit (Tissue) enables rapid and crude isolation of of intact mitochondria from animal tissues from both soft and hard tissues using differential centrifugation. This crude mitochondrial preparation is often enough for most applications, such as study of mitochondrial respiration, mitochondria membrane potential, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling etc.
Kit components	• Lysis Buffer A (5×) • Lysis Buffer B (5×) • Storage Buffer
Features & Benefits	• Isolate intact mitochondria from animal tissues from both soft and hard tissues. • Isolated mitochondria is compatible with almost any downstream application.
Usage notes	Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Storage instructions	Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Shipping	Gel pack with blue ice.