總黃麴毒素檢測試劑盒 | AF (Total Aflatoxin) ELISA Kit 貨號 FSES0073
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總黃麴毒素檢測試劑盒AF (Total Aflatoxin) ELISA Kit (FSES0073) 貨號 FSES0073
Grain—0.1 ppb; Formula feed—0.2 ppb; Edible oil,Peanut—0.2 ppb; Biscuit —0.2 ppb; Beer—0.2 ppb; Wine, Soy sauce, Vinegar—0.1 ppb
Total AF (Total Aflatoxin ) ELISA Kit 總黃麴毒素檢測試劑盒 (貨號FSES0075)。該試劑盒使用競爭ELISA方法(Competitive-ELISA method)。 可檢測樣品中的總麴毒霉毒素（Aflatoxin），如穀物(Cereals)、飼料(formula feed)、食用油(edible oil)等。該試劑盒由ELISA微量滴定板組成(ELISA Microtiter plate)，HRP 偶聯物(HRP conjugate) 、抗體工作液(antibody working solution)、標準品(standard)和其他補充試劑。 這本試劑盒中的微量滴定板(microtiter plate)已預先包被(pre-coated)有偶聯抗原(antigen)。 在反應過程中，樣品或標準品中的 AF與固相上的偶聯抗原競爭抗 AF抗體位點的supporter。 然後辣根過氧化物酶 (Horseradish Peroxidase , HRP) 偶聯物是添加到每個微量滴定板孔中，並添加底物試劑(substrate reagent) 進行顯色。樣品OD值與樣品濃度呈負相關(negative correlation)。 樣品中 AF的濃度可以通過比較 OD樣品到標準曲線。
AF (Total Aflatoxin) ELISA Kit
| Kit Name: | AF (Total Aflatoxin) ELISA Kit |
| Kit Size: | 96 Assays |
| Test Principle: | This kit is a competitive enzyme immunoassay for the quantitative determination of aflatoxins in cereals, feed, etc. |
| Sensitivity: | Contact Technical Support |
| Reaction Mode: | 25°C, 30 min~ 15 min |
| Detection Limit: | Grain—0.1 ppb; Formula feed—0.2 ppb; Edible oil,Peanut—0.2 ppb; Biscuit —0.2 ppb; Beer—0.2 ppb; Wine, Soy sauce, Vinegar—0.1 ppb |
| Cross Reactivity: | Aflatoxin B1 (AFB1) —100%, AflatoxinB2 (AFB2) —80%, Aflatoxin G1 (AFG1)–75%, Aflatoxin G2 (AFG2) —45%, Aflatoxin M1 (AFM1) —8% |
| Sample Recovery Rate: | |
| Kits Components: | Contact Technical Support |
| Instruments: | Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01g). Please see protocol for additional equipment requirements. |
| Micropipette: | Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL). |
| Reagents: | Contact Technical Support |
| Storage And Expiry Date: | Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 – 8°C. Expiry date: expiration date is on the packing box. |
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