Human SGLT2 (Sodium/glucose cotransporter 2) ELISA Kit (HUFI03401)貨號HUFI03401

SGLT2 (Sodium/glucose cotransporter 2) encodes a sodium-dependent glucose transport protein, which is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in SGLT2 (Sodium/glucose cotransporter 2) are associated with renal glucosuria and aminoaciduria. The Assay Genie Human SGLT2 (Sodium/glucose cotransporter 2) ELISA is a highly sensitive assay for the quantitative measurement of SGLT2 (Sodium/glucose cotransporter 2) in serum, blood, plasma, cell culture supernatant and tissue samples.

SGLT2（Sodium/glucose cotransporter 2, 鈉/葡萄糖協同轉運蛋白 2）編碼鈉依賴性葡萄糖轉運蛋白(sodium-dependent glucose transport protein)，它是參與腎臟葡萄糖重吸收(glucose reabsorption) 的主要協同轉運蛋白(cotransporter)。 SGLT2（鈉/葡萄糖協同轉運蛋白 2）的突變(Mutations)與腎性糖尿(renal glucosuria)和氨基酸尿(aminoaciduria)有關。 Assay Genie Human SGLT2（鈉(Sodium )/葡萄糖協同轉運蛋白 2(glucose cotransporter 2)）ELISA 是一種高度靈敏的測定方法，用於定量測量血清、血液、血漿、細胞培養上清液和組織樣品中的 SGLT2（鈉/葡萄糖協同轉運蛋白 2）

Human SGLT2 (Sodium/glucose cotransporter 2) ELISA

SGLT2（Sodium/glucose cotransporter 2）ELISA試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的SGLT2。偵測範圍: 15.625-1000pg/ml, 最低靈敏度9.375pg/ml。可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

ELISA Microplate (Dismountable)	8×12 strips	4°C for 6 months
Lyophilized Standard	2	4°C/-20°C
Sample/Standard Dilution Buffer	20ml	4°C
Biotin-labeled Antibody(Concentrated)	120ul	4°C (Protect from light)
Antibody Dilution Buffer	10ml	4°C
HRP-Streptavidin Conjugate(SABC)	120ul	4°C (Protect from light)
SABC Dilution Buffer	10ml	4°C
TMB Substrate	10ml	4°C (Protect from light)
Stop Solution	10ml	4°C
Wash Buffer(25X)	30ml	4°C
Plate Sealer	5	–

 

KU:

HUFI03401

PRODUCT TYPE:

ELISA Kit

SIZE:

96 Assays

UNIPROT:

P31639

SENSITIVITY:

9.375pg/ml

RANGE:

15.625-1000pg/ml

ELISA TYPE:

Sandwich ELISA, Double Antibody

SYNONYMS:

Sodium/glucose cotransporter 2, Na(+)/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, Solute carrier family 5 member 2, SLC5A2, SGLT2

REACTIVITY:

Human

Product Name:	Human SGLT2 (Sodium/glucose cotransporter 2) ELISA Kit
Product Code:	HUFI03401
Size:	96 Assays
Alias:	Sodium/glucose cotransporter 2 ELISA Kit, Na(+)/glucose cotransporter 2 ELISA Kit, Low affinity sodium-glucose cotransporter ELISA Kit, Solute carrier family 5 member 2 ELISA Kit, SLC5A2 ELISA Kit, SGLT2 ELISA Kit
Detection method:	Sandwich ELISA, Double Antibody
Application:	This immunoassay kit allows for the in vitro quantitative determination of Human SGLT2 (Sodium/glucose cotransporter 2) concentrations in serum plasma and other biological fluids.
Sensitivity:	< 9.375pg/ml
Range:	15.625-1000pg/ml
Storage:	4°C for 6 months
Note:	For Research Use Only

 

Component	Quantity	Storage
ELISA Microplate (Dismountable)	8×12 strips	4°C for 6 months
Lyophilized Standard	2	4°C/-20°C
Sample/Standard Dilution Buffer	20ml	4°C
Biotin-labeled Antibody(Concentrated)	120ul	4°C (Protect from light)
Antibody Dilution Buffer	10ml	4°C
HRP-Streptavidin Conjugate(SABC)	120ul	4°C (Protect from light)
SABC Dilution Buffer	10ml	4°C
TMB Substrate	10ml	4°C (Protect from light)
Stop Solution	10ml	4°C
Wash Buffer(25X)	30ml	4°C
Plate Sealer	5	–

 

Sample Type	Protocol
Serum	If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma	Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid	Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant	Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates	Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates	The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates	Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk	Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.