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抗體矩陣Human Obesity Array (62 targets) |Assaygenie (SARB0015)

Assaygenie提供一系列抗體矩陣(Antibody Array )產品, 抗體矩陣(Antibody Array )是一種基於 ELISA 的高通量平台，Human Obesity Array (62 targets) (貨號SARB0015) 同時檢測樣本內多個Biomarker的含量 (62種相關蛋⽩) ACE2, ADIPOQ, AGRP, ANGPT1, ANGPT2, ANGPTL4, CCL16, CCL18, CCL2, CCL25| CCL4, CCL5, CCL7, CFD, CRP, CSF1, CXCL10, CXCL12, CXCL5, CXCL8, EDA2R, FAS, FGF6, GH1, IFNG, IGF1, IGF1R, IGFBP1, IGFBP2, IGFBP3, IL10, IL11, IL12A, IL1A, IL1B, IL1R1, IL1RL1, IL6, IL6R, INS, LEP, LEPR, LIF, MIF, MST1, OSM, PDGFA, PDGFB, RETN, SAA1, SERPINE1, TGFB1, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF1A, TNFRSF1B, TNFRSF9, TNFSF9, VEGFA, XCL1)。用於在細胞、組織、血清甚至培養基中進行有效的蛋白質表達譜分析。與蛋白質印跡法(Western Blots)或傳統 ELISA 等傳統方法相比，抗體陣列檢測的生產效率和成本效益要高得多。步驟簡單，使用方便，無需專用設備。

Product Sku:	SARB0015
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	62
Gene Symbols:	ACE2, ADIPOQ, AGRP, ANGPT1, ANGPT2, ANGPTL4, CCL16, CCL18, CCL2, CCL25|CCL4, CCL5, CCL7, CFD, CRP, CSF1, CXCL10, CXCL12, CXCL5, CXCL8, EDA2R, FAS, FGF6, GH1, IFNG, IGF1, IGF1R, IGFBP1, IGFBP2, IGFBP3, IL10, IL11, IL12A, IL1A, IL1B, IL1R1, IL1RL1, IL6, IL6R, INS, LEP, LEPR, LIF, MIF, MST1, OSM, PDGFA, PDGFB, RETN, SAA1, SERPINE1, TGFB1, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF1A, TNFRSF1B, TNFRSF9, TNFSF9, VEGFA, XCL1
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

 

Targets Included
4-1BB (TNFRSF9/CD137)	ACE-2	Adiponectin (ACRP30)	Adipsin (Complement Factor D)	AgRP
Angiopoietin-1	Angiopoietin-2	ANGPTL4	CRP (C-Reactive Protein)	ENA-78 (CXCL5)
Fas (TNFRSF6/Apo-1)	FGF-6	Growth Hormone	HCC-4 (CCL16)	IFN-gamma
IGFBP-1	IGFBP-2	IGFBP-3	IGF-1	IGF-1 R
IL-1 R4 (ST2)	IL-1 R1	IL-10	IL-11	IL-12 p70
IL-1 alpha (IL-1 F1)	IL-1 beta (IL-1 F2)	IL-6	IL-6 R	IL-8 (CXCL8)
Insulin	IP-10 (CXCL10)	Leptin R	Leptin	LIF
Lymphotactin (XCL1)	MCP-1 (CCL2)	MCP-3 (MARC/CCL7)	M-CSF	MIF
MIP-1 beta (CCL4)	MSP alpha/beta	Osteoprotegerin (TNFRSF11B)	Oncostatin M	PAI-1
PARC (CCL18)	PDGF-AA	PDGF-AB	PDGF-BB	RANTES (CCL5)
Resistin	SAA (Serum Amyloid A)	SDF-1 alpha (CXCL12 alpha)	TNF RII (TNFRSF1B)	TNF RI (TNFRSF1A)
TECK (CCL25)	TGF beta 1	TIMP-1	TIMP-2	TNF alpha
VEGF-A	XEDAR

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

• Human Obesity Antibody Array C1 Membranes
• Blocking Buffer
• Wash Buffer 1
• Wash Buffer 2
• Biotinylated Detection Antibody Cocktail
• Streptavidin-Conjugated HRP
• Detection Buffer C
• Detection Buffer D
• Lysis Buffer
• 8-Well Incubation Tray
• Plastic Sheets
• Array Templates

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis