assaygenie 食品安全ELISA Kit系列-Competitive-ELISA Kit for Kanamycin

KAM (Kanamycin) ELISA Kit (FSES0059)​ 卡那黴素（Kanamycin,KAM）檢測試劑盒(競爭型酵素免疫分析法) | 貨號FSES0059

assaygenie提供高靈敏度之Competitive-ELISA (競爭型酵素免疫分析套組)，靈敏度 0.3 ppb (ng/mL)、最低靈敏度Meat, Milk —10 ppb; Honey —20 ppb.。本試劑盒採用競爭ELISA方法。 可檢測肉類、蜂蜜等樣品中的卡那黴素（Kanamycin,KAM）。該試劑盒由ELISA微量滴定板、HRP偶聯物、抗體工作液、標準品及其他補充試劑組成。 該試劑盒中提供的微量滴定板已預先塗有偶聯抗原。 在檢測過程中，樣品或標準品中的（Kanamycin,KAM）與固相支持物上的偶聯抗原競爭抗（Kanamycin,KAM）抗體的位點。 然後向每個孔中加入辣根過氧化物酶 (HRP) 偶聯物，並加入底物試劑進行顯色。 樣品OD值與（Kanamycin,KAM）濃度呈負相關。 通過將樣品的 OD 與標準曲線進行比較，可以計算出樣品中 （Kanamycin,KAM） 的濃度。

Kit Name:	KAM (Kanamycin) ELISA Kit
Kit Size:	96 Assays
Test Principle:	This kit uses Competitive-ELISA as the method. It can detect Kanamycin (KAM) in samples, such as meat, honey, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, KAM in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-KAM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of KAM. The concentration of KAM in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity:	0.3 ppb (ng/mL)
Reaction Mode:	25°C,30 min~15min
Detection Limit:	Meat, Milk —10 ppb; Honey —20 ppb.
Cross Reactivity:	Kanamycin—100%; Neomycin,Streptomycin,Gentamicin—<0.1%
Sample Recovery Rate:	90%±30%.
Instruments:	Microplate reader, Oscillators, Centrifuge, Balance (sensibility 0.01g).
Micropipette:	Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents:	Trichloroacetic Acid (C2HCl3O2).
Storage And Expiry Date:	Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 – 8°C. Expiry date: expiration date is on the packing box.

 

Item	Specifications
ELISA Microtiter Plate	96 wells
Standard Liquid	1 mL each (0 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb, 24.3 ppb)
HRP Conjugate	7 mL
Antibody Working Solution	7 mL
10×Concentrated Sample Diluent	25 mL
Substrate Reagent A	6 mL
Substrate Reagent B	6 mL
Stop Solution	6 mL
20×Concentrated Wash Buffer	25 mL
Plate Sealer	3 pieces
Sealed Bag	1 piece
Manual	1 copy