25-hydroxy vitamin D3 ELISA試劑盒|  25-hydroxy vitamin D3 ELISA貨號KTE60833

Human 25-hydroxy vitamin D3 (25 HVD3) ELISA Kit

骨化二醇(Calcifediol)是一種前激素，由肝臟中維生素 D3（膽鈣化醇）的羥基化產生。然後骨化二醇(Calcifediol)在腎臟中（通過酶 25(OH)D-1α-羥化酶）轉化為骨化三醇 (1,25-(OH)2D3)，一種是維生素 D 活性形式的類固醇激素。它也可以是通過 24-羥基化作用在腎臟中轉化為 24-羥基骨化二醇。在醫學上，骨化醇的血濃度被認為是維生素 D 狀態的最佳指標。這是最敏感的措施，儘管專家呼籲提高不同實驗室的標準化和可重複性。正常範圍因多種因素而異，包括年齡和地理位置。已建議 20–150 nmol/L 的廣泛參考範圍，而幾項研究已將低於 80 nmol/L 的水平定義為維生素 D 缺乏的指示

This Human 25-hydroxy vitamin D3 (25 HVD3) ELISA Kit employs a two-site sandwich ELISA to quantitate 25 HVD3 in samples. An antibody specific for 25 HVD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any25 HVD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 25 HVD3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 25 HVD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.。

Product name	Human 25-hydroxy vitamin D3 (25 HVD3) ELISA Kit
Reactivity	Human
Applications	ELISA
Detection method	Colorimetric
SampleType	Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type	Sandwich ELISA (quantitative)
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative	25 HVD3

使用注意事項

• 請勿混用不同試劑盒中的組分，或在試劑盒到期日期之後使用試劑。
• 打開之前，讓所有試劑至少加室溫30分鐘。
• 用試劑預清洗移液器吸頭，為每個樣品，標準液和試劑使用新鮮的移液器吸頭，以免污染。
• 未使用的孔必須在提供的密封袋中於4°C乾燥。
• 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
• 建議所有樣品和標準品一式兩份或一式三份進行測定。