cTnT人類心臟亞型肌鈣蛋白 T ELISA試劑盒 | 貨號KTE60186

Human Cardiac isoform of Tropnin T (cTnT) ELISA Kit

人類心臟亞型肌鈣蛋白 T (Human Cardiac isoform of Tropnin T, cTnT) ELISA 試劑盒採用two-site sandwich ELISA來定量樣品中的 TNNT2。 TNNT2 特異性抗體已預先包被到微孔板上。標準品和樣品被移入孔中，任何存在的 TNNT2 都被固定化抗體結合。去除任何未結合的物質後，將特異於 TNNT2 的生物素偶聯抗體添加到孔中。洗滌後，將鏈黴親和素偶聯的辣根過氧化物酶 (HRP) 添加到孔中。洗滌以去除任何未結合的親和素酶試劑後，將底物溶液加入孔中，顏色與初始步驟中結合的 TNNT2 的量成比例。

• 請勿混用不同試劑盒中的組分，或在試劑盒到期日期之後使用試劑。
• 打開之前，讓所有試劑至少加室溫30分鐘。
• 用試劑預清洗移液器吸頭，為每個樣品，標準液和試劑使用新鮮的移液器吸頭，以免污染。
• 未使用的孔必須在提供的密封袋中於4°C乾燥。
• 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
• 建議所有樣品和標準品一式兩份或一式三份進行測定。

Kit components	• Human Cardiac isoform of Tropnin T microplate • Human Cardiac isoform of Tropnin T standard • Human Cardiac isoform of Tropnin T detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Features & Benefits	Human Cardiac isoform of Tropnin T (cTnT) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNNT2. No significant cross-reactivity or interference between Human TNNT2 and analogues was observed.
Calibration range	Please inquire
Limit of detection	Please inquire
Usage notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions	The unopened kit should be stored at 2 – 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.