GMS 染色試劑盒 | Modified Gomori Methenamine-Silver Nitrate Stain貨號KAA-1 (125ML) 

Modified Gomori Methenamine-Silver Nitrate Stain（GMS 染色試劑盒）旨在用於真菌(fungi)、基底膜(basement membrane)和一些機會性生物（如卡氏肺囊蟲, Pneumocystis carinii）的組織學可視化。卡氏肺囊蟲是一種機會性病原體，可導致嚴重的肺病人類、狗、大鼠、小鼠和其他脊椎動物的疾病，具有獲得性、誘發性或遺傳性免疫缺陷綜合徵。此外，此程序將演示放線菌和相關種、諾卡氏菌小行星和某些包囊細菌。

Procedure (Standard):

1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in Chromic Acid Solution for 10 minutes.
3. Rinse in tap water followed by 2 changes of distilled water.
4. Incubate slide in Sodium Bisulfite Solution for 1 minute (to remove any residual chromic acid).
5. Rinse in tap water followed by 2 changes of distilled water.
6. Combine the following for a working GMS solution: 25 ml Silver Nitrate Solution (0.2%) 25 ml Methenamine Solution 2 ml Borax Solution Note: Mixed solution may not be stored for reuse later.
7. Place working GMS solution in 60° centigrade water bath and allow temperature to equilibrate.
8. Incubate slide in working GMS solution for 10-15 minutes. Using plastic forceps, dip slide in distilled water and check under a microscope for evaluation of silver impregnation. Fungi should be dark brown. If color is not sufficient, return the slide to working GMS solution for 2-3 minutes and check again.
9. Rinse in 4 changes of distilled water.
10. Incubate slide in Gold Chloride Solution for 15-30 seconds.
11. Rinse in 4 changes of distilled water.
12. Incubate slide in Sodium Thiosulfate Solution (5%) for 2 minutes.
13. Rinse in tap water followed by 2 changes of distilled water.

14. Incubate slide in Light Green Solution for 2 minutes.
15. Rinse slide using absolute alcohol.
16. Dehydrate in 2 changes of absolute alcohol, clear, and mount in synthetic resin. Note: Increasing incubation time and temperature of the Chromic Acid Solution reduces background silver staining. We suggest a 10min incubation at 60°C if there is unwanted background staining.

Procedure (Microwave):

Note: These instructions were developed using a standard 500 watt microwave oven. Heating times should be modified as needed depending on the microwave oven used. 1. Deparaffinize sections if necessary and hydrate to distilled water.

2. Place slide in plastic coplin jar filled with Chromic Acid solution. Cap jar loosely!
3. Place jar in microwave oven and heat on high power for 10 seconds. Allow slide to remain in warm solution for 3 minutes.
4. Rinse in tap water followed by 2 changes of distilled water.
5. Incubate slide in Sodium Bisulfite solution for 1 minute (to remove any residual chromic acid).
6. Rinse in tap water followed by 2 changes of distilled water.
7. Combine the following for a working GMS solution: 25 ml Silver Nitrate 25 ml Methenamine 2 ml Borax Solution Note: Mixed solution may not be stored for reuse later.
8. Place working GMS solution (loosely capped) in microwave oven for 40 seconds. Remove and pour several times between coplin jar and a clear graduated cylinder to mix thoroughly (use protective glove to avoid burning hand). Mixed solution remains in coplin jar.
9. Incubate slide in working GMS solution (heated) for 2-6 minutes until the tissue is medium brown in color. Using plastic forceps, dip slide in distilled water and check under a microscope for evaluation of silver impregnation. Fungi should be dark brown. If color is not sufficient, return the slide to working GMS solution for 1-2 minutes and check again. Reheat solution if needed.
10. Rinse in 4 changes of distilled water.
11. Incubate slide in Gold Chloride solution for 15-30 seconds.
12. Rinse in 4 changes of distilled water.
13. Incubate slide in Sodium Thiosulfate for 2 minutes.
14. Rinse in tap water followed by 2 changes of distilled water.
15. Incubate slide in Light Green Solution for 2 minutes.
16. Rinse slide using absolute alcohol.

Scytek品牌介紹 原廠連結

ScyTek Laboratories成立於1991年，其目標是成為生產生命科學試劑的世界級製造公司。從早期生產ELISA試劑開始，ScyTek逐漸擴大產品線，包括IHC試劑，特殊染色，緩沖液，Mounting Media，固定液，稀釋液和各種客製化試劑等等。從一開始，ScyTek就採用了嚴格的原料篩選流程，以確保所有製造的產品都是最高品質。2011年，ScyTek實驗室獲得了ISO 9001-2008認證，在2016年獲得了ISO 13485認證。

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