Human CD274/PD-L1 protein, His tag (Animal-Free)重組蛋白 |貨號PRP2021

程序性死亡-1配體-1（Programmed death-1 ligand-1, PD-L1、CD274、B7-H1）已被鑑定為免疫抑制受體程序性死亡-1（immunoinhibitory receptor programmed death-1, PD1/PDCD1）的配體，並已被證明在免疫調節(immune responses)中發揮作用。反應和外周耐受。在誘導(induction)和維持(maintenance)對自身的免疫耐受中起關鍵作用。作為抑制性受體 PDCD1/PD-1 的配體(ligand)，調節 T 細胞的激活閾值(activation threshold)並限制 T 細胞效應反應(T-cell effector response)。通過未知的激活受體，可共刺激主要產生白細胞介素 10 (IL10) 的 T 細胞亞群(T-cell subsets)。 PDCD1 介導的抑制通路被腫瘤利用來減弱抗腫瘤免疫並逃避免疫系統的破壞，從而促進腫瘤存活。與 PDCD1/PD-1 的相互作用抑制細胞毒性 T 淋巴細胞 (CTL) 效應子功能（相似性）。 PDCD1 介導通路的阻斷導致耗竭 T 細胞表型的逆轉和抗腫瘤反應(anti-tumor response)的正常化，為癌症免疫治療提供了基本原理。

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產品介紹: Human CD274/PD-L1 protein, His tag (Animal-Free)重組蛋白 ， 由HEK293 Cells生產，胺基酸序列源自人 CD274 (NP_054862.1) (Met1-Thr239) 的氨基酸序列，在 C 端帶有 6 His 標籤。重組人 CD274蛋白， 由 227 個氨基酸組成，預計分子量為 26.8 KD。由於糖基化(glycosylation)，它在還原條件下在 SDS-PAGE 中遷移為大約 33-40 KD。純度>98% (determined by SDS-PAGE); 內毒素(Endotoxin): < 1.0 EU per µg of the protein ( determined by the LAL method)。Alternative Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1

Fig. SDS-PAGE analysis of Human CD274/PD-L1 protein, His tag.

Product name	Human CD274/PD-L1 protein, His tag (Animal-Free)
Sequence	Amino acid sequence derived from human CD274 (NP_054862.1) (Met1-Thr239)was expressed with a 6 His tag at the C-terminus.
Protein length	The recombinant human CD274 consists of 227 amino acids and predicts a molecular mass of 26.8 KD. It migrates as an approximately 33-40 KD band in SDS-PAGE under reducing conditions due to glycosylation.
Preparation method	HEK293 Cells
Purity	>98% as determined by SDS-PAGE
Alternative	Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1
Formulation	Lyophilized from sterile PBS, pH 7.4.
Features & Benefits	Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.
Molecular weight	26.8 KD
Usage notes	Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD274/PD-L1 protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions	Lyophilized Human CD274 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping	The product is shipped at ambient temperature.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Gene ID	29126
Alternative	Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1
Accession	Q9NZQ7