Minute™新鮮/冷凍組織胞質胞核分離試劑盒 | 貨號 NT-032
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Minute™新鮮/冷凍組織胞質胞核分離試劑盒 | 貨號 NT-032
Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues
產品介紹: 從細胞和組織中分離細胞質(cytosolic)和細胞核組分(nuclear fractions)是一種常見的實驗。細胞樣品的組分分離相對簡單直接，然而，從組織樣品中特別是冷凍組織(frozen tissues)中將胞質和胞核組分分離乾淨是很具挑戰的。因為冷凍和反復凍融會引起組織細胞結構的改變，加之不適當的勻漿方式和效率低的提取緩衝液，使得交叉污染十分嚴重。目前大多數商業試劑盒設計可同時用於細胞和組織樣品，但是忽略了兩種類型樣品之間的結構差異。因此，從新鮮/冷凍組織中分離胞質和胞核組分時，交叉污染問題無法避免(見下文參考文獻1和2)。這款新型的胞質和胞核分離試劑盒專用於組織樣品的胞質胞核分離，試劑盒特有的緩衝液體系和獨特的操作方法可以將交叉污染降到最低。整個操作可以在30分鐘內完成。
應用：
可應用於SDS-PAGE，immunoblottings，ELISA，IP，蛋白定位，凝膠遷移分析，2-D等其他應用。
儲存：
4℃
Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性。無須長時間等待、免除重複性的wash步驟。
引用文獻
1.Li, Y., Wu, J., Yu, S., Zhu, J., Zhou, Y., Wang, P., … & Zhao, Y. (2020). Sestrin2 promotes angiogenesis to alleviate brain injury by activating Nrf2 through regulating the interaction between p62 and Keap1 following photothrombotic stroke in rats.Brain Research, 146948.
2.Wang, B., Huang, X., Pan, X., Zhang, T., Hou, C., Su, W. J., … & Wang, Y. X. (2020). Minocycline prevents the depressive-like behavior through inhibiting the release of HMGB1 from microglia and neurons.Brain, Behavior, and Immunity.
3.Song T, Guan X, Wang X,et al. Dynamic modulation of gut microbiota improves post-myocardial infarct tissue repair in rats via butyric acid-mediated histone deacetylase inhibition.The FASEB Journal. 2021;35:e21385.
4.Zhou, J., Zhao, Y., Guo, Y. J., Zhao, Y. S., Liu, H., Ren, J., … & Ji, E. S. (2021). A rapid juvenile murine model of nonalcoholic steatohepatitis (NASH): Chronic intermittent hypoxia exacerbates Western diet-induced NASH. Life Sciences, 119403.
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| Minute TM Plasma Membrane Protein Isolation Kit (50 Tests) | SM-005 |
| Minute TM Plasma Membrane Protein Isolation Kit for Plants (50 Tests) | SM-005-p |
| Minute TM Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests) | MP-007 |
| Minute TM Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells (50 Tests) NEW! | MM-038 |
| Minute TM Total Protein Extraction Kit for Plant Tissues (50 Tests) | SD-008/SN-009 |
| Minute TM Detergent-Free Plant Protein Extraction Kit (50 Tests) | SN-010 |
| Minute TM Chloroplast Isolation Kit (50 Tests) | CP-011 |
| Minute TM Bacterial Total Protein Extraction Kit (50 Tests) | SB-004 |
| Minute TM Single Cell Isolation Kit (50 Tests) | SC-012 |
| Minute TM Nuclear Envelope Protein Extraction Kit (50 Tests) | NE-013 |
| Minute TM Histon/DNA Binding Protein Extraction Kit (50 Tests) | HP-014 |
| Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) | YT-015 |
| Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) | YD-016 |
| Minute™ Yeast Mitochondria Enrichment Kit (50 tests) | YM-017 |
| Minute™ Plant Microsomal Membrane Extraction Kit (50 Tests) | MM-018 |
| Minute™ Protein/Nucleic Acid Extraction Kit from Gels (20 tests) | PN-019 |
| MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests) | HD-021 |
| MinuteTM Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Tests) | AT-022 |
| MinuteTM Adipose Tissue Fractionation Kit (20 Tests) | AF-023 |
| MinuteTM Detergent-Free Nuclei Isolation Kit (20 Tests) | NI-024 |
| MinuteTM Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps) NEW! | BN-020 |
| MinuteTM Single Nucleus Isolation Kit for Tissues/Cells (20 preps) NEW! | SN-047 |
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| MinuteTM Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG) | SE-030 |
| MinuteTM Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 tests) | NT-032 |
| MinuteTM Lysosome Isolation Kit (20 tests) | LY-034 |
| MinuteTM ER Enrichment Kit (20 tests) | ER-036 |
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| MinuteTM Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) NEW! | ML-044 |
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| MinuteTM Total Protein Extraction Kit for Skin Tissue (50 tests) | SA-01-SK |
| MinuteTM Total Protein Extraction Kit for Bone Tissue (50 tests) | SA-02-BT |
| MinuteTM Total Protein Extraction Kit for Blood Vessels (50 tests) | SA-03-BV |
| MinuteTM Total Protein Extraction Kit for Tendons (50 tests) | SA-04-TD |
| MinuteTM Total Protein Extraction Kit for Intestines and Mesentery (50 tests) | SA-05-IM |
| MinuteTM Total Protein Extraction Kit for Muscles (50 tests) | SA-06-MS |
| MinuteTM Total Protein Extraction Kit for Insects (50 tests) | SA-07-IS |
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