DR5/TRAIL R2 Protein, Human, Recombinant (His Tag), Biotinylated | 貨號10465-H08H-B

腫瘤壞死因子受體超家族，成員1b(TNFRSF1B)，也稱為死亡受體5，CD262，TNF相關凋亡誘導配體受體2 （TNF-related apoptosis-inducing ligand receptor 2, TRAIL R2），是TNF受體超家族的成員，包含細胞內死亡領域。該受體可以被腫瘤壞死因子相關的凋亡誘導配體（tumor necrosis factor-related apoptosis inducing ligand , TNFSF1 / TRAIL / APO-2L）激活，並轉導凋亡信號。對FADD缺陷小鼠的研究表明，FADD（一種含有銜接蛋白的死亡域）是該蛋白介導的凋亡所必需的。 TRAIL R2 / CD262 / TNFRSF1B作為在兩個不同的人類細胞系表面上可檢測到的TRAIL的唯一受體被獨立純化，該細胞系在受到TRAIL刺激後發生凋亡。 TRAIL R2 / CD262 / TNFRSF1B包含兩個富含胞外半胱氨酸的重複序列，通常是TNF受體（TNFR）家族成員和一個細胞質死亡域。 TRAIL R2 / CD262 / TNFRSF1B通過細胞內銜接子分子FADD / MORT1介導凋亡。 TRAIL受體既可以發出死亡信號，又可以發出基因轉錄信號，其功能讓人想起TNFR1和TRAMP（死亡受體家族的另外兩個成員）的功能。 TRAIL R2 / CD262 / TNFRSF1B中的缺陷可能是引起頭頸部鱗狀細胞癌（neck squamous cell carcinomas , HNSCC）的原因，也被稱為頭頸部鱗狀細胞癌(carcinoma of the head and neck)。

 產品描述: TNFRSF10B（NP_003833.3）細胞外結構域（Met1-Glu182）的DNA序列。C端具HIS，純化的蛋白質在體外被生物素化。A DNA sequence encoding the human TNFRSF10B (NP_003833.3) extracellular domain (Met1-Glu182) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

Purity

> 95 % as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the human TNFRSF10B (NP_003833.3) extracellular domain (Met1-Glu182) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

Accession#

NP_003833.3

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Ile 56

Molecule Mass

The recombinant human TNFRSF10B consists of 138 amino acids and predicts a molecular mass of 15.8 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

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