DR5/TRAIL R2 Protein, Mouse, Recombinant (His Tag)  | 貨號50412-M08H

產品描述: DR5/TRAIL R2 Protein, Mouse, Recombinant (His Tag). 分泌的重組小鼠TNFRSF10B由136個氨基酸組成，計算的分子量為15 kDa。由於糖基化，在還原條件下，SDS-PAGE中重組蛋白的表觀分子量約為25-35 kDa。

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Immobilized mouse TNFRSF10B-His at 10 μg/ml (100 μl/well) can bind biotinylated human TNFSF10 (Cat:10409-HNAE), The EC₅₀ of biotinylated human TNFSF10 (Cat:10409-HNAE) is 0.16-0.38 μg/ml.

Protein Construction

A DNA sequence encoding the extracellular domain of mouse TNFRSF10B (NP_064671.2) (Met 1-Ser 177) was expressed, with a C-terminal polyhistidine tag.

Accession#

NP_064671.2

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Asn 53

Molecule Mass

The secreted recombinant mouse TNFRSF10B consists of 136 amino acids and has a calculated molecular mass of 15 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 25-35 kDa in SDS-PAGE under reducing conditions.

 

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