神經元組織/細胞Minute™單核分離試劑盒| Invent貨號 BN-020
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產品名稱: 神經元組織/細胞Minute™單核分離試劑盒（20次製備）| Invent貨號 BN-020
Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps)
該試劑盒可用於從大多數神經元組織/培養細胞（新鮮或冷凍）中分離細胞核
Nuclei Isolated from zebrafish brain tissue Using BN-020 and stained with DAPI (False-coloured in green) (Courtesy Sema Elif Eski, Singh Lab, Université Libre de Bruxelles).
產品介紹: 分離的核(nuclei)廣泛用於各種實驗，例如FACS，單核分析（single nucleus analysis, 例如RNA-seq和ATAC-seq），免疫螢光染色(immunofluorescence staining)，細胞週期(cell cycle)和細胞凋亡研究(apoptosis research)。單細胞RNA-seq(Single cell RNA-seq)是研究組織複雜細胞組成的powerful技術。然而，神經元(Neurons)是高度互連的，並且很難從諸如大腦(brain)和脊髓(spinal cord)的神經元組織(neuronal tissues)獲得單細胞。從冷凍的神經元組織(frozen neuronal tissues)中分離完整細胞甚至更加困難。由於這些限制，單細胞RNA-seq(single cell RNA-seq)被單核-seq取代。從神經元組織中分離單核的傳統方法相對繁瑣且耗時，並且由於難以擺脫污染的髓磷脂(myelin)和其他細胞成分，因此收率通常較低。該套件專門設計用於通過簡單，快速和有效的操作方法。大約30分鐘即可獲得高度純化的單核。與傳統方法相比，該試劑盒需要的起始原料少得多，並且可以處理大範圍的樣品量（1-25 mg）。緩衝液包含專用的detergents混合物，可有效裂解細胞。如果不希望使用去污劑，也可以使用貨號NI-024的無去污劑核分離試劑盒。
Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性。無須長時間等待、免除重複性的wash步驟。
Kit includes:
| Items | Quantity |
| Buffer A | 15 ml |
| Buffer B | 25 ml |
| Pestles for 1.5 ml Tubes | 2 units |
| Filter Cartridge with Collection Tubes | 20 units |
| Product Name | Catalog No. |
| Minute TM Total Protein Extraction Kit for Animal Cultured Cells/Tissues (50 Tests) | SD-001/SN-002 |
| Minute TM Cytoplasmic and Nuclear Fractionation kit (50 Tests) | SC-003 |
| Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (50 Tests) | SN-006 |
| Minute TM Plasma Membrane Protein Isolation Kit (50 Tests) | SM-005 |
| Minute TM Plasma Membrane Protein Isolation Kit for Plants (50 Tests) | SM-005-p |
| Minute TM Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests) | MP-007 |
| Minute TM Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells (50 Tests) NEW! | MM-038 |
| Minute TM Total Protein Extraction Kit for Plant Tissues (50 Tests) | SD-008/SN-009 |
| Minute TM Detergent-Free Plant Protein Extraction Kit (50 Tests) | SN-010 |
| Minute TM Chloroplast Isolation Kit (50 Tests) | CP-011 |
| Minute TM Bacterial Total Protein Extraction Kit (50 Tests) | SB-004 |
| Minute TM Single Cell Isolation Kit (50 Tests) | SC-012 |
| Minute TM Nuclear Envelope Protein Extraction Kit (50 Tests) | NE-013 |
| Minute TM Histon/DNA Binding Protein Extraction Kit (50 Tests) | HP-014 |
| Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) | YT-015 |
| Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) | YD-016 |
| Minute™ Yeast Mitochondria Enrichment Kit (50 tests) | YM-017 |
| Minute™ Plant Microsomal Membrane Extraction Kit (50 Tests) | MM-018 |
| Minute™ Protein/Nucleic Acid Extraction Kit from Gels (20 tests) | PN-019 |
| MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests) | HD-021 |
| MinuteTM Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Tests) | AT-022 |
| MinuteTM Adipose Tissue Fractionation Kit (20 Tests) | AF-023 |
| MinuteTM Detergent-Free Nuclei Isolation Kit (20 Tests) | NI-024 |
| MinuteTM Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps) NEW! | BN-020 |
| MinuteTM Single Nucleus Isolation Kit for Tissues/Cells (20 preps) NEW! | SN-047 |
| MinuteTM Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests) | FE-025 |
| MinuteTM Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG) | EI-027 |
| MinuteTM Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG) | SE-030 |
| MinuteTM Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 tests) | NT-032 |
| MinuteTM Lysosome Isolation Kit (20 tests) | LY-034 |
| MinuteTM ER Enrichment Kit (20 tests) | ER-036 |
| MinuteTM Lipid Raft Isolation Kit for Mammalian Cells/Tissues (20 tests) NEW! | LR-039 |
| MinuteTM Lipid Raft Isolation Kit for Mammalian Cells/Tissues (20 tests) NEW! | LR-042 |
| MinuteTM Cytosolic Proteasome Enrichment Kit (20 tests) NEW! | PT-040 |
| MinuteTM Nuclear Proteasome Enrichment Kit (20 tests) NEW! | PN-041 |
| MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests) NEW! | PF-045 |
| MinuteTM Native Protein Precipitation Kit from Saliva (20 tests) NEW! | SV-043 |
| MinuteTM Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) NEW! | ML-044 |
| MinuteTM Rubisco Depletion Kit (20 tests) NEW! | RD-046 |
| MinuteTM Total Protein Extraction Kit for Skin Tissue (50 tests) | SA-01-SK |
| MinuteTM Total Protein Extraction Kit for Bone Tissue (50 tests) | SA-02-BT |
| MinuteTM Total Protein Extraction Kit for Blood Vessels (50 tests) | SA-03-BV |
| MinuteTM Total Protein Extraction Kit for Tendons (50 tests) | SA-04-TD |
| MinuteTM Total Protein Extraction Kit for Intestines and Mesentery (50 tests) | SA-05-IM |
| MinuteTM Total Protein Extraction Kit for Muscles (50 tests) | SA-06-MS |
| MinuteTM Total Protein Extraction Kit for Insects (50 tests) | SA-07-IS |
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