卵清蛋白(Ovalbumin) ELISA試劑盒| Chicken Ovalbumin (OVA) ELISA Kit貨號KTE30160

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卵清蛋白(Ovalbumin) ELISA試劑盒| Chicken Ovalbumin (OVA) ELISA Kit貨號KTE30160
產品描述:卵清蛋白(Ovalbumin) ELISA試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的卵清蛋白(Ovalbumin)。對卵清蛋白(Ovalbumin)特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中,並且存在的任何纖維蛋白原都被固定的抗體結合。除去所有未結合的物質後,將HRP卵清蛋白(Ovalbumin)檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的卵清蛋白(Ovalbumin)的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 312.5ng/L-5000ng/L, 最低靈敏度30ng/mL。可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum
卵清蛋白(Ovalbumin)是蛋清中的主要蛋白質,佔總蛋白質的60-65%。卵清蛋白(While Ovalbumin)顯示與絲氨酸蛋白酶抑制劑超家族的序列和三維同源性(three-dimensional homology),但它是一種非抑制性絲氨酸蛋白酶抑制劑。儘管大多數絲氨酸蛋白酶抑制劑通過抑制絲氨酸蛋白酶來控制纖維蛋白溶解(fibrinolysis)和凝血等過程,但卵白蛋白的功能尚不清楚,儘管它被認為是一種storage protein。
- Product name
Chicken Ovalbumin (OVA) ELISA Kit
- Reactivity
Chicken
- Applications
ELISA
- Application notes
Chicken Ovalbumin (OVA) ELISA Kit employs a two-site sandwich ELISA to quantitate Ovalbumin in samples. An antibody specific for Ovalbumin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ovalbumin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate ChickenOvalbumin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Ovalbumin bound in the initial step. The color development is stopped and the intensity of the color is measured.
- Detection method
Colorimetric
- Sample type
Other biological fluids, Plasma, Serum
- Assay type
Sandwich ELISA (quantitative)
- Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 2-148 hours. It depends on the experience of the operation person.
- Calibration range
312.5ng/L-5000ng/L
- Limit of detection
30 ng/L
- Usage notes
- Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least175 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for
使用注意事項
- 請勿混用不同試劑盒中的組分,或在試劑盒到期日期之後使用試劑。
- 打開之前,讓所有試劑至少加室溫30分鐘。
- 用試劑預清洗移液器吸頭,為每個樣品,標準液和試劑使用新鮮的移液器吸頭,以免污染。
- 未使用的孔必須在提供的密封袋中於4°C乾燥。
- 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
- 建議所有樣品和標準品一式兩份或一式三份進行測定。
