核酸外切酶III(Exonuclease III) | 貨號E016 10000 U (100 μl)

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原廠連結：	https://www.abmgood.com
相關下載：	核酸外切酶III操作手冊

特點

abm提供了一系列核酸酶 nucleases)，可製備RNA和DNA，以便在下一代全基因組測序(whole genome sequencing)和文庫構建(library construction)中用於後續應用。

核酸外切酶III(Exonuclease III)，大腸桿菌從切口DNA(nicked DNA)，鈍端DNA(blunt end DNA)，3′-凹陷末端(3′-recessed ends)或少於4個鹼基的3′-突出端沿3’→5’方向消化雙鏈體DNA，並生成核苷5′-磷酸。 DNA降解以均勻的速度進行，並在相反的鏈上產生ssDNA片段。在確定的反應條件下，反應可產生可預測和可再現結果。需要優化條件，例如溫度(temperature)，離子強度(ionic strength)，模板DNA(template DNA)序列和核酸外切酶III與DNA的比例，以適合特定應用以實現所需的切除率。由於固有的3′-磷酸酶活性(3′-phosphatase activity)，外切核酸酶III(Exonuclease III)，大腸桿菌也能夠從3′-磷酸末端降解DNA。該酶還具有嘌呤DNA核酸內切酶活性以及RNase H活性。

Description	Exonuclease III, E.coli digests duplex DNA in the 3′→5′ direction from nicked DNA, blunt end DNA, 3′-recessed ends, or 3′-overhangs of less than four bases, and yields nucleoside 5′-phosphates. The DNA degradation proceeds at a uniform rate and produces stretches of ssDNA on the opposite strand. Under defined reaction conditions, the reaction can yield predictable and reproducible digestion results. Conditions such as temperature, ionic strength, template DNA sequence and the Exonuclease III to DNA ratio need to be optimized to suit specific applications to achieve the desired excision rate. Exonuclease III, E.coli is also capable of degrading DNA from 3′-phosphate ends due to intrinsic 3′-phosphatase activity. The enzyme also has apurinic DNA endonuclease activity as well as RNase H activity.
SKU	E016
Unit quantity	10 000 U (100 μl)
Applications	Generation of intermediates for site-directed mutagenesis Preparation of strand-specific radio labelled probes Preparation of single-stranded DNA Preparation of single-stranded templates for dideoxy-sequencing of DNA Creation of unidirectional deletions in DNA fragments Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Concentration	100 U/μl
Format	Enzyme supplied with 10X Reaction Buffer
Storage Condition	Store all components at -20°C.
Storage Buffer	20 mM Tris-HCl (pH 7.5), 100 mM KCl, 1 mM DTT, and 50% (v/v) Glycerol.

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