RNase III, E.coli | 貨號E020 100 U (100 μl)

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原廠連結：	https://www.abmgood.com
相關下載：	RNase III, E.coli操作手冊

特點

abm提供了一系列核酸酶 nucleases)，可製備RNA和DNA，以便在下一代全基因組測序(whole genome sequencing)和文庫構建(library construction)中用於後續應用。

RNase III，大腸桿菌是一種內切核糖核酸酶(endoribonuclease)特異性地將dsRNA消化成12-15個鹼基對(12-15 base pairs )sRNA片段，該片段具有2個或3個核苷酸的3′-突出端(3′-overhangs)有5’磷酸(5′ phosphate )’羥基末端(3′ hydroxyl termini ) RNase III

Description	RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3′-overhangs with 5′ phosphate and 3′ hydroxyl termini.
SKU	E020
Unit quantity	100 U (100 μl)
Applications	• dsRNA digestion to short dsRNA fragments • Useful for RNA processing, maturation and structure studies • Directed cleavage of RNA • siRNA preparation for RNA interference in mammalian cellsNote: This product is not suitable for NGS or second strand cDNA synthesis applications.
Concentration	1 U/μl
Format	Enzyme supplied with 10X Reaction Buffer
Storage Condition	Store at -20°C.
Storage Buffer	50 mM Tris-HCl (pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
Note	One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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